Bovine estrogen receptor binds chromatin at pre-existing nuclease hypersensitive sites

Pratt, K; Wierowski, James V.; Hilf, Russell; Bambara, Robert A.

doi:10.1016/0303-7207(84)90018-2

Cited by 5 publications

(2 citation statements)

References 36 publications

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“…4, 5 but cf. [6][7][8] or in equilibrium between the cytoplasm and nucleus (9). However, results of a variety of studies (10,11) suggested that unoccupied and occupied estrogen receptors (ERs) and progestin receptors (PRs) are present almost exclusively in cell nuclei, and they suggested that the unoccupied receptors typically found in cytosol after cell fractionation and differential centrifugation result from tissue disruption.…”

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confidence: 99%

Colchicine‐lnduced Accumulation of Estrogen Receptor and Progestin Receptor Immunoreactivity in Atypical Areas in Guinea‐Pig Brain

Blaustein

Olster

1993

J Neuroendocrinology

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Using immunocytochemical techniques, cells containing estrogen and progestin receptors have been observed in many discrete regions of the guinea-pig forebrain, including the mediobasal hypothalamus and preoptic area. While most reaction product is located within cell nuclei, we have reported abundant reaction product in perikaryal cytoplasm and neuronal processes as well. Ultrastructural analysis has revealed the presence of estrogen and progestin receptors in atypical subcellular sites within the hypothalamus, including dendrites and axon terminals. In order to determine if microtubule-dependent intracellular transport is involved in intraneuronal transport of steroid hormone receptors, ovariectomized guinea-pigs were injected intracerebroventricularly with the microtubule inhibitor, colchicine, and brain sections at the level of the hypothalamus were immunostained for estrogen receptors. This treatment resulted in the appearance of estrogen receptor immunoreactivity in the paraventricular and mediodorsal thalamic region, areas typically devoid of estrogen receptor-immunoreactive cells in guinea-pigs. In a second study on progestin receptors, we observed the colchicine-induced accumulation of progestin receptor immunoreactivity in the paraventricular thalamic, mediodorsal thalamic and lateral dorsal thalamic areas as well as in the medial amygdala, all areas typically devoid of progestin receptor immunoreactivity. While estradiol injection induced progestin receptor immunoreactivity in the hypothalamus and preoptic area as described previously, it had no effect on the colchicine-induced accumulation in the thalamus and amygdala. These results provide evidence that in some neurons, progestin receptors and estrogen receptors are transported intracellularly, apparently at a rapid enough rate that they do not ordinarily accumulate within the perikaryon.

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confidence: 99%

Colchicine‐lnduced Accumulation of Estrogen Receptor and Progestin Receptor Immunoreactivity in Atypical Areas in Guinea‐Pig Brain

Blaustein

Olster

1993

J Neuroendocrinology

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“…DHSs are signatures of chromatin accessibility to regulators of gene expression [109,110]. For example, bovine estrogen receptor bind to pre-existing nuclease hypersensitive sites in chromatin [111]. Furthermore, distal DHSs correspond to regulatory elements that upregulate or repress gene expression [109].…”

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confidence: 99%

Defining Candidate Imprinted loci in Bos taurus

Bina

2023

Genes

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Using a whole-genome assembly of Bos taurus, I applied my bioinformatics strategy to locate candidate imprinting control regions (ICRs) genome-wide. In mammals, genomic imprinting plays essential roles in embryogenesis. In my strategy, peaks in plots mark the locations of known, inferred, and candidate ICRs. Genes in the vicinity of candidate ICRs correspond to potential imprinted genes. By displaying my datasets on the UCSC genome browser, one could view peak positions with respect to genomic landmarks. I give two examples of candidate ICRs in loci that influence spermatogenesis in bulls: CNNM1 and CNR1. I also give examples of candidate ICRs in loci that influence muscle development: SIX1 and BCL6. By examining the ENCODE data reported for mice, I deduced regulatory clues about cattle. I focused on DNase I hypersensitive sites (DHSs). Such sites reveal accessibility of chromatin to regulators of gene expression. For inspection, I chose DHSs in chromatin from mouse embryonic stem cells (ESCs) ES-E14, mesoderm, brain, heart, and skeletal muscle. The ENCODE data revealed that the SIX1 promoter was accessible to the transcription initiation apparatus in mouse ESCs, mesoderm, and skeletal muscles. The data also revealed accessibility of BCL6 locus to regulatory proteins in mouse ESCs and examined tissues.

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Progesterone-Modulation of Estrogen Action: Rapid Down Regulation of Nuclear Acceptor Sites for the Estrogen Receptor

Leavitt

Cobb

Takeda

1987

Advances in Experimental Medicine and Biology

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Bovine estrogen receptor binds chromatin at pre-existing nuclease hypersensitive sites

Cited by 5 publications

References 36 publications

Colchicine‐lnduced Accumulation of Estrogen Receptor and Progestin Receptor Immunoreactivity in Atypical Areas in Guinea‐Pig Brain

Colchicine‐lnduced Accumulation of Estrogen Receptor and Progestin Receptor Immunoreactivity in Atypical Areas in Guinea‐Pig Brain

Defining Candidate Imprinted loci in Bos taurus

Progesterone-Modulation of Estrogen Action: Rapid Down Regulation of Nuclear Acceptor Sites for the Estrogen Receptor

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