Bovine embryo technologies

Galli, Cesare; Duchi, Roberto; Crotti, G.; Turini, Paola; Ponderato, Nunzia; Colleoni, Silvia; Lagutina, Irina; Lazzari, Giovanna

doi:10.1016/s0093-691x(02)01243-8

Cited by 153 publications

(139 citation statements)

References 59 publications

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“…For Czech Siemmental, Holstein Dairy and Beef Cattle cows, the mean proportion of transferable embryos derived from oocytes recovered in the growth phase of follicular development were 23.6%, 21.6% and 13.3%, respectively. These values are comparable with those reported by Galli et al (2003) who obtained embryo development rates of 21.8% and 15.6% in oocytes collected from culled genetically valuable cows and heifers of one herd, respectively, and by Merton et al (2007) who reached embryo development rates of 23.4 to 24.7% in oocytes collected by transvaginal aspiration from healthy pregnant heifers and first-parity Holstein Friesian cows.…”

Section: Discussionsupporting

confidence: 89%

In vitro production of embryos from high performance cows and the development of frozen-thawed embryos after transfer: a field study

Machatkova¹,

Hulinska²,

Rečková³

et al. 2008

Vet. Med. - Czech

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AbstrAct:In this field study, embryos were derived from genetically highly valuable cows excluded from breeding due to reproductive disorders. Cows, 5 to 10 years old, of Czech Siemmental, Holstein Dairy and Beef Cattle breeds were used as oocyte donors. Oocytes were obtained either in the growth phase of the first follicular wave from cows with synchronized oestrus or in any other phases of follicular development from cows without oestrus synchronization. The embryos were prepared by a standard protocol described previously. The mean number of usable oocytes, transferable and freezable embryos per donor, and the mean percentage of usable, transferable and freezable embryos were assessed. The results were analyzed by Student's-t and Chi-squared tests. The embryos were frozen according to a slow freezing protocol. After thawing, they were transferred to recipients on Day 7 after oestrus. Irrespective of the breed, the mean numbers of usable oocytes and transferable and freezable embryos collected per donor were significantly higher (P < 0.01) for the synchronized than for the nonsynchronized donors (20.4 vs 11.7, 4.3 vs 1.0 and 3.2 vs 0.8, respectively). Similarly, the mean percentages of usable oocytes, transferable and freezable embryos were significantly higher (P < 0.01) for the synchronized than for the nonsynchronized donors (28.5% vs 20.5%, 20.9% vs 9.0% and 15.8% vs 6.5%, respectively). On comparison of the synchronized and nonsynchronized donors of each breed, the difference in the mean percentage of usable oocytes was significant (P < 0.01) in cows of all three breeds, the difference in the mean percentage of transferable embryos was significant in Czech Siemmental and Holstein Dairy cows (P < 0.01) and the difference in the mean percentage of freezable embryos was significant only in Holstein Dairy cows (P < 0.01). After the transfer of 41 frozen-thawed embryos and 43 fresh embryos, 20 heifers and 24 heifers became pregnant, respectively. In conclusion: (a) higher number of oocytes from infertile, genetically valuable cows was recovered in the growth phase compared with the other phases of follicular development; (b) greater development of these oocytes resulted in more embryos for transfer and cryopreservation; (c) the transfer of frozen-thawed and fresh embryos resulted in pregnancy rates of 48.8% and 55.8% , respectively.

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Section: Discussionsupporting

confidence: 89%

In vitro production of embryos from high performance cows and the development of frozen-thawed embryos after transfer: a field study

Machatkova¹,

Hulinska²,

Rečková³

et al. 2008

Vet. Med. - Czech

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“…Madrid (2003), evaluando toros con diferentes tasas de no retorno (alta, media y baja), tampoco encontró diferencias entre toros en la producción de blastocistos y en el desarrollo de los embriones, pero los porcentajes de fertilidad y de producción de blastocistos fueron mayores. No obstante, otros autores reportan valores similares a los del presente estudio (Alberio, 2003;Galli, 2003). Asimismo, la tasa de desarrollo de blastocistos en base al total de ovocitos madurados y al de ovocitos fertilizados fue similar a los resultados encontrados por Palma (2001), lo cual indica que en la última década, de un 20 a 40% de los ovocitos seleccionados para maduración in vitro alcanzan estadios de blastocistos.…”

Section: Resultados Y Discusiónunclassified

EVALUACIÓN DE LA FERTILIDAD in vitro DEL SEMEN DE TOROS JÓ- VENES NACIONALES EN OVOCITOS PROVENIENTES DE OVARIOS DE ANIMALES BENEFICIADOS

L.³

2012

Rev. investig. vet. Perú

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“…Today routine protocols for bovine are widely established and allow the use of bovine embryo technologies, besides its classical use in animal breeding, as research tool for transgenesis (Galli et al, 2003).…”

Section: Historymentioning

confidence: 99%

“…Improvement of in vitro production techniques of bovine embryos faciliates the application of this technique to address fundamental questions of the endocrine control, the molecular switches and the metabolic pathways that regulate early embryonic development (Galli et al, 2003). Therefore numerous reports exist, where this research tool is used to obtain information about transgenesis, gene targeting and NT in bovine in vitro (Iguma et al, 2005, Cho et al, 2004, Arat et al, 2002, Wells and Powell, 2000, Rosochacki et al, 2003.…”

Section: Future Prospectsmentioning

confidence: 99%