Bovine dilated cardiomyopathy: Proteomic analysis of an animal model of human dilated cardiomyopathy

Weekes, John R.; Wheeler, Colin; Yan, Jun X.; Weil, Joachim; Eschenhagen, Thomas; Scholtysik, Gnter; Dünn, Michael J.

doi:10.1002/9783527613489.ch42

Cited by 22 publications

(30 citation statements)

References 34 publications

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“…A disorder in the lipid metabolism might be an important characteristic of stress-induced dysfunction of mitochondria. The present data are consistent with the observations of Heinke et al (1999) and Weekes et al (1999) in experimental models of pacing-induced canine heart failure and bovine dilated cardiomyopathy. Glucose-regulated protein is a member of the HSP70 superfamily.…”

Section: Discussionsupporting

confidence: 82%

“…Three protein bands were found to contain NGFI-B, all of which were successfully identified. Most of the interesting proteins were mitochondrial proteins related to energy production (Kotyza et al 2009;Lee et al 2009;Heinke et al 1999;Weekes et al 1999). In this work, we detected mitochondrial respiratory function, ATP synthase activity, and ATP content in cardiomyocytes by biochemical methods.…”

Section: Discussionmentioning

confidence: 95%

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NGFI-B targets mitochondria and induces cardiomyocyte apoptosis in restraint-stressed rats by mediating energy metabolism disorder

Wang¹,

Liu²,

Kong³

et al. 2009

Cell Stress and Chaperones

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NGFI-B/Nur77/TR3, originally identified as an immediate-early gene rapidly induced by serum and growth factors, is a member of the steroid hormone nuclear receptor superfamily with no identified endogenous ligand. NGFI-B induces apoptosis in a number of cell lineages exposed to proapoptotic stimuli by directly targeting the mitochondria, inducing cytochrome c release. The present study was designed to determine the role of NGFI-B in cardiomyocytes of restraintstressed rats. The NGFI-B content was increased in mitochondria and reduced in plasma as apoptosis increased. Analysis showed that NGFI-B induces cardiomyocyte apoptosis in restraint-stressed rats by mediating mitochondrial energy metabolism disorder. Several novel mitochondrial proteins, which correlate with NGFI-B, were reported in cardiomyocyte apoptosis of restraint-stressed rats. Five proteins associated with NGFI-B participate directly in mitochondrial energy metabolism. Studies of mitochondrial respiratory efficiency and ATP synthase activity strongly support the findings. These results provide significant information for comprehensively understanding the cellular mechanism of cardiovascular diseases.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 95%

NGFI-B targets mitochondria and induces cardiomyocyte apoptosis in restraint-stressed rats by mediating energy metabolism disorder

Wang¹,

Liu²,

Kong³

et al. 2009

Cell Stress and Chaperones

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show abstract

“…(reviewed in [48,49]) Majority of mam-malian intracellular proteins are degraded via the UPS, and UPS is believed to play a role in the replacement of myofilament proteins subjected to wear and tear [49]. Interestingly, UPS activity increased in an animal model of dilated cardiomyopathy (DCM), and protein ubiquitination was markedly increased [50]. In a follow-up proteomic study of human patients with DCM, several ubiquitinated heart proteins, including MHC fragment, were identified [51].…”

Section: Degradationmentioning

confidence: 99%

Myofilament proteins: From cardiac disorders to proteomic changes

Yuan

Solaro

2008

Proteomics Clinical Apps

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Myofilament proteins of the cardiac sarcomere house the molecular machinery responsible for generating tension and pressure. Release of intracellular Ca 21 triggers myofilament tension generation and shortening, but the response to Ca 21 is modulated by changes in key regulatory proteins. We review how these proteomic changes are essential to adaptive physiological regulation of cardiac output and become maladaptive in cardiac disorders. We also review the essentials of proteomic techniques used to study myofilament protein changes, including degradation, isoform expression, phosphorylation and oxidation. Selected proteomic studies illustrate the applications of these approaches.

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“…The proteins were prepared and separated as described previously (Weekes et al, 1999). Isoelectric focusing was performed on nonlinear 3 -10 IPG strips, 7 cm in size.…”

Section: Methodsmentioning

confidence: 99%

Proteome analysis enables separate clustering of normal breast, benign breast and breast cancer tissues

Dwek

Alaiya

2003

Br J Cancer

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Bovine dilated cardiomyopathy: Proteomic analysis of an animal model of human dilated cardiomyopathy

Cited by 22 publications

References 34 publications

NGFI-B targets mitochondria and induces cardiomyocyte apoptosis in restraint-stressed rats by mediating energy metabolism disorder

NGFI-B targets mitochondria and induces cardiomyocyte apoptosis in restraint-stressed rats by mediating energy metabolism disorder

Myofilament proteins: From cardiac disorders to proteomic changes

Proteome analysis enables separate clustering of normal breast, benign breast and breast cancer tissues

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