Bovine brain coated vesicles contain guanine nucleotide regulatory proteins.

Moroi, Kayoko; Kuga, Tetsurō; Kadota, Ken

doi:10.1254/jjp.55.399

Cited by 3 publications

(4 citation statements)

References 18 publications

(11 reference statements)

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“…Both stimulatory and inhibitory G-proteins have been associated with coated vesicle frac-lin-and GABA-immunoreactive neurons suggest that the inhibitory effects of opiates in the subfornical organ may be mediated or potentiated by GABA (Tongroach et al, 1984). tions in brain (Moroi et al, 1991). Multivesicular bodies seen near postsynaptic junctions also may indicate sites of receptor internalization and degradation (Schmied and Holtzman , 1987).…”

Section: Synaptic Input To Unlabeled and Gaba-labeled Dendritesmentioning

confidence: 99%

Met⁵‐enkephalin is localized within axon terminals in the subfornical organ: Vascular contacts and interactions with neurons containing gamma‐aminobutyric acid

Pickel¹,

Chan²

1994

J of Neuroscience Research

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Met5-enkephalin inhibits sodium and water excretion and antagonizes the central actions of angiotensin II in subfornical organ of rat brain. We examined the ultrastructural basis for enkephalin modulation in this circumventricular region. Additionally, we examined the possibility that there might be cellular sites for functional interactions involving Met5-enkephalin and gamma-aminobutyric acid (GABA), a known inhibitory transmitter throughout the central nervous system. Met5-enkephalin and GABA were identified in single coronal sections through the subfornical organ using immunoperoxidase and silver-enhanced immunogold labeling methods, respectively. Enkephalin-like immunoreactivity was most prominently localized within axon terminals. These were distributed primarily in the central, highly vascular, regions of the subfornical organ. Enkephalin-labeled terminals were apposed to the basement membranes of fenestrated capillaries and also formed symmetric, inhibitory type synapses with neurons. In terminals associated with either blood vessels or neurons, the enkephalin immunoreactivity was enriched in large (80-150 nm) dense core vesicles. The immunoreactive vesicles were usually located within portions of the axon in close proximity to astrocytic processes. In contrast, smaller vesicles in the same terminals were more often aggregated near the basement membrane of the capillaries and the active zone of the synapse. The targets of enkephalin-immunoreactive terminals were either unlabeled or GABA-labeled dendrites of local neurons. Enkephalin was also co-localized with GABA in perikarya and in axon terminals. Terminals containing only GABA were far more abundant than those containing enkephalin or enkephalin and GABA. GABA-immunoreactive terminals formed symmetric synapses on unlabeled dendrites some of which also received convergent input from terminals containing enkephalin. Additionally, the enkephalin-immunoreactive terminals were closely apposed to GABA-labeled and unlabeled terminals. These results suggest sites for nonsynaptic release of Met5-enkephalin from dense core vesicles in contact with astrocytes near blood vessels and synaptic complexes in the rat subfornical organ. Moreover, the observed dual localization and pre- and postsynaptic associations between neurons containing Met5-enkephalin and GABA indicate that inhibitory effects of opioids in the subfornical organ may be mediated or potentiated by GABA.

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Section: Synaptic Input To Unlabeled and Gaba-labeled Dendritesmentioning

confidence: 99%

Met⁵‐enkephalin is localized within axon terminals in the subfornical organ: Vascular contacts and interactions with neurons containing gamma‐aminobutyric acid

Pickel¹,

Chan²

1994

J of Neuroscience Research

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“…[3H]GTP binding assay was performed as described by Moroi et al (1991). The CVs (35 wg of protein) were incubated at 0°C for 45 min with various concentrations (1-IS nM) of ['H]GTP in a total volume of 200~1 of 50 mM Tris-HCl buffer, pH 7.4.…”

Section: Radioligand Binding Assaymentioning

confidence: 99%

“…ADP ribosylation of CVs with [3zP]NAD and CTX or IAP was performed according to the method of Neer et al (1987) and Moroi et al (1991). The CVs were incubated with [3ZP]NAD (2~Ci/tube) in the absence or the presence of IAP (2.5 wg/tube)/CTX (10 wg/tube) at 25°C for 1 h in a final volume of 100~l of reaction mixture .…”

Section: Adp Ribosylation and Immunoblotting Of G Proteinsmentioning

confidence: 99%

“…Some of these receptors are coupled with effector systems in bovine brain CVs, i.e ., adenosine A, receptors coupled with G; protein to adenylate cyclase; metabotropic glutamate receptors coupled with phospholipase C via G protein; muscarinic acetylcholine receptors coupled with G protein (Silva et al ., 1986;González-Calero et al ., 1990, 1992Ros et al ., 1990;Martín et al ., 1991 b). In addition, high-affinity binding sites for [3H]GTP and both G; (M~39,000-40,000) and GS (M~45,000 and 52,000) proteins were found in CVs from bovine cerebral cortex (Moroi et al ., 1991 ;Silva, 1991) .…”

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Dopamine D₁ and D₂ Receptors and Their Signal System Present in Coated Vesicles Prepared from Bovine Striatal Tissue

Ozaki

Moroi

Kadota

et al. 1994

Journal of Neurochemistry

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Coated vesicles (CVs) isolated from bovine striatal tissue were examined to determine whether they are associated with dopamine signal systems consisting of dopamine D, and D Z receptors, G proteins, and adenylate cyclase . Dopamine receptors in CVs were characterized by a dopamine D, receptor antagonist, [3 H]SCH 23390, and a dopamine D2 receptor antagonist, [ 3 H]spiroperidol . The bindings of both ligands were specifically saturable and reversible with a dissociation constant (K p) of 0 .65 and 0 .5 nM, respectively . Dopaminergic antagonists and agonists inhibited the specific bindings of [ 3H]SCH 23390 and [3 H]spiroperidol in a stereoselective and concentration-dependent manner with an appropriate rank order potency for dopamine D, or D 2 receptors . The regulations of the agonist binding by guanyl-5-ylimidodiphosphate were observed . ADP ribosylation of the CVs with [32 P]NAD demonstrated predominant labeling of bands of M, 47,000-52,000, 42,000-45,000, and 40,000-39,000, which corresponded to the known molecular weights of the a subunits of GS and G ; proteins . The presence of a and ß subunits of G proteins in the CVs was also confirmed by immunoblotting assay . Adenylate cyclase activity, which was stimulated by SKF 38393 and inhibited by dopamine D Z receptor agonists, was present in the CVs . These findings suggest that the dopamine D,

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Characterization of [3H]5-hydroxytryptamine and [3H]spiperone binding sites in clathrin-coated vesicles from bovine brain

et al. 1998

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Bovine brain coated vesicles contain guanine nucleotide regulatory proteins.

Cited by 3 publications

References 18 publications

Met⁵‐enkephalin is localized within axon terminals in the subfornical organ: Vascular contacts and interactions with neurons containing gamma‐aminobutyric acid

Met⁵‐enkephalin is localized within axon terminals in the subfornical organ: Vascular contacts and interactions with neurons containing gamma‐aminobutyric acid

Dopamine D₁ and D₂ Receptors and Their Signal System Present in Coated Vesicles Prepared from Bovine Striatal Tissue

Characterization of [3H]5-hydroxytryptamine and [3H]spiperone binding sites in clathrin-coated vesicles from bovine brain

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Bovine brain coated vesicles contain guanine nucleotide regulatory proteins.

Cited by 3 publications

References 18 publications

Met5‐enkephalin is localized within axon terminals in the subfornical organ: Vascular contacts and interactions with neurons containing gamma‐aminobutyric acid

Met5‐enkephalin is localized within axon terminals in the subfornical organ: Vascular contacts and interactions with neurons containing gamma‐aminobutyric acid

Dopamine D1 and D2 Receptors and Their Signal System Present in Coated Vesicles Prepared from Bovine Striatal Tissue

Characterization of [3H]5-hydroxytryptamine and [3H]spiperone binding sites in clathrin-coated vesicles from bovine brain

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Met⁵‐enkephalin is localized within axon terminals in the subfornical organ: Vascular contacts and interactions with neurons containing gamma‐aminobutyric acid

Met⁵‐enkephalin is localized within axon terminals in the subfornical organ: Vascular contacts and interactions with neurons containing gamma‐aminobutyric acid

Dopamine D₁ and D₂ Receptors and Their Signal System Present in Coated Vesicles Prepared from Bovine Striatal Tissue