Bovine and buffalo in vitro embryo production using oocytes derived from abattoir ovaries or collected by transvaginal follicle aspiration

Neglia, Gianluca; Gasparrini, B.; Brienza, V. Caracciolo di; Palo, R. Di; Campanile, Giuseppe; Presicce, Giorgio Antonio; Zicarelli, L.

doi:10.1016/s0093-691x(02)01170-6

Cited by 106 publications

(79 citation statements)

References 25 publications

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“…In contrast with these results the earlier studies by Neglia et al (2003) yielded blastocyst rate of 19.9% in control group. The discrepancies in the blastocyst yield may be attributed to the quality of oocytes obtained from abattoir and culture conditions.…”

Section: Discussioncontrasting

confidence: 95%

Effect of all-trans retinol on in vitro development of preimplantation buffalo embryos

Thiyagarajan

Valivittan

2009

Animal

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Vitamin A is a well-known antioxidant and is essential for embryonic development, growth and differentiation. Oxidative stress is involved in the etiology of defective embryo development. The present study evaluated whether the presence of all-trans retinol (0, 1, 2, 5 and 10 mM) in maturation medium or embryo culture medium would enhance the developmental competence of preimplantation buffalo embryos in vitro. In experiment I, cumulus oocytes complex were matured with varying concentrations of all-trans retinol. Treatment with 5 mM all-trans retinol improved the blastocyst formation ( P , 0.001) when compared with control and significant increase ( P , 0.01) in total cell number was observed in 5 mM group when compared with control. Supplementation of all-trans retinol in embryo culture medium for the entire culture period under 5% O 2 and 20% O 2 was tested in experiments II and III, respectively. Supplementation of 10 mM all-trans retinol under 5% O 2 , significantly reduced blastocyst formation and cell numbers. Presence of 5 mM all-trans retinol under 20% O 2 enhanced the frequency of blastocyst formation and total cell number ( P , 0.001) when compared with control. DNA damage of individual embryos cultured under 20% oxygen concentration was measured by the comet assay. Supplementation of 5 mM all-trans retinol significantly reduced the comet tail ( P , 0.001) when compared with control. Supplementation of all-trans retinol in embryo culture medium for first 72 h of the 8-day culture period under 5% O 2 was tested in experiment IV. Addition of 5 mM all-trans retinol resulted in significant increase in blastocyst rate and total cell number ( P , 0.001) when compared with control. Our results demonstrate that addition of all-trans retinol to maturation or embryo culture medium may enhance the developmental competence of buffalo embryos in vitro by enhancing blastocyst formation rate and total cell number.

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Section: Discussioncontrasting

confidence: 95%

Effect of all-trans retinol on in vitro development of preimplantation buffalo embryos

Thiyagarajan

Valivittan

2009

Animal

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“…The higher number of COC recruited per donor in bovine vs buffalo results from the different follicular population of the two species. It was also observed, confirming previous findings (Neglia et al, 2003), that the percentage of good quality oocytes is higher in bovine compared to buffalo. However, it is worth pointing out that the average number of COC/donor recorded in buffalo in the present trial is higher than usually reported in literature (Gasparrini, 2002).…”

Section: Materials and Methods -supporting

confidence: 91%

Ovum pick-up and in vitro production technology in field conditions in the North East of Argentina

Pellerano¹,

Gasparrini²,

Crudeli

et al. 2007

Italian Journal of Animal Science

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ABSTRACT:The aim of this work was to assess the feasibility of ovum pick-up and in vitro embryo production technology in field conditions in buffalo and crossbreed cattle. This study represents the first trial of in vitro fertilization carried out in North East Argentina. Buffalo (n=11) and bovine (n=3) donors underwent Ovum pick-up twice per week for 2 weeks. The equipment was hand-made, resulting in the impossibility of controlling the aspiration pressure. A higher number of cumulus-oocyte-complexes were recovered in bovine vs buffalo (6.3 vs 3.6 per donor respectively). In bovine the quality of the COCs was also superior (53.9 vs 26.6 % of highest quality COCs in bovine and buffalo respectively). A significantly higher incidence of denuded oocytes was found in buffalo (38.0 vs 11.8 %), indicating a higher sensitivity of buffalo oocytes to mechanical damages. Despite the poor oocyte quality and the suboptimal culture conditions, the latter related to the lack of lab facilities, buffalo embryo cleaved (35.7 %) and developed up to the tight-morula stage (22.5 %). This preliminary trial sets the basis for future studies in Argentina.

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“…This land mark achievement led to production of a large number of buffalo embryos and pregnancies from abattoir-derived ovaries, but the success rate was low (Gasparrini 2002;De Rosa et al 2005;Madan 2005;Hufana-Duran et al 2007). In comparison to cattle, buffaloes show a similar maturation rate (94 vs 87 %, respectively), but a significantly lower cleavage rate (84 vs 65 %, respectively) (Gasparrini 2002;Neglia et al 2003). Also, the blastocyst rate of buffalo is poor (20 %) compared to cattle (35-48 %) (Liang et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

et al. 2016

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The objective of this study was to compare effects of in vitro culture systems on embryonic development and expression patterns of developmentally important genes in preimplantation buffalo embryos. After IVM/IVF presumptive zygotes were cultured in one of three systems: undefined TCM-199, mCR2aa medium supplemented with 10 % FBS and defined PVA-myo-inositol-phosphate-EGF medium. No (P [ 0.05) differences at 2-cell, 4-cell and 8-cell to 16-cell stages were observed among the three cultured media used, however, increased (P \ 0.05) blastocyst yield, cell number and hatching rate were found in defined medium compared to undefined media. The expression patterns of genes implicated in embryo metabolism (GLUT-1), anti-apoptosis (BCL-2), imprinting (IGF-2R), DNA methylation (DNMT-3A) and maternal recognition of pregnancy (IFNT) were increased (P \ 0.05) in hatched blastocysts derived from defined medium compared to undefined media. In conclusion, serum-free, defined medium improved developmental competence of in vitro cultured buffalo embryos. Whether these differences in morphological development and gene expression have long-term effects on buffalo calves born after embryo transfer remains unknown. However, it is possible that early adaptations of the preimplantation embryo to its environment persist during fetal and post-natal development.

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Bovine and buffalo in vitro embryo production using oocytes derived from abattoir ovaries or collected by transvaginal follicle aspiration

Cited by 106 publications

References 25 publications

Effect of all-trans retinol on in vitro development of preimplantation buffalo embryos

Effect of all-trans retinol on in vitro development of preimplantation buffalo embryos

Ovum pick-up and in vitro production technology in field conditions in the North East of Argentina

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

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