Bovine anaplasmosis and tick-borne pathogens in cattle of the Galapagos Islands

Gioia, Gisela Vasconcelos; Vinueza, Rommel Lenin; Marsot, Maud; Devillers, Elodie; Cruz, Marilyn; Petit, Élisabeth; Boulouis, Henri-Jean; Moutailler, Sara; Monroy, Fernando P.; Coello, M. A.; Gondard, Mathilde; Bournez, Laure; Haddad, Nadia; Zanella, Gina

doi:10.1111/tbed.12866

Cited by 19 publications

(12 citation statements)

References 43 publications

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“…Previous studies in Ecuador demonstrated the presence of Babesia spp. in ticks, but not in cattle [16,18]. Our study shows for the first time B. bovis and B. bigemina in two geographical areas El Carmen and Quito, with a prevalence of 18.93% and 20.28%, respectively for Babesia spp.…”

Section: Discussionmentioning

confidence: 58%

“…(21%) [16]. In addition, molecular tests as Polymerase Chain Reaction (PCR) and quantitative polymerase chain reaction (qPCR) demonstrated the presence of B. bovis, B. bigemina, and mixed infections in ticks (R. microplus) [16][17][18]. On the contrary, the use of a PCR test using ribosomal 18s as a target (PCR) with primers described by Carret et al, 1999 [19], did not demonstrate the presence of Babesia spp.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Babesia spp. in High Altitude Cattle in Ecuador, Possible Evidence of the Adaptation of Vectors and Diseases to New Climatic Conditions

et al. 2021

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Background: Babesia species are intraerythrocytic protozoa, distributed in tropical and subtropical areas of the world, causing anemic diseases in many animals, including cattle. This disease, called babesisosis, is transmitted from one animal to another through ticks (Tick Borne-Disease or TBD). On the other hand, Ecuador has a tropical climate that allows the development of the vector Rhipicephalus microplus, and therefore favors the transmission of Babesia spp. in cattle. Methods and principal findings: We determined the presence of Babesia spp. by PCR using 18s ribosomal gene as target (18s PCR) in 20 farms in the area of El Carmen (zone below 300 m above sea level) and 1 farm in Quito (2469 m.a.s.l.). In addition, we analyzed parameters such as age, sex, and packed cell volume (PCV) as explanatory variable associated with the disease. Results: The 18s PCR test showed that 18.94% (14.77% Babesia bovis and 4.17% Babesia bigemina) and 20.28% (14.69% B. bovis and 5.59% B. bigemina) of the cattle were positive for Babesia spp in farms sampled in El Carmen and in Quito, respectively. Age influenced the presence of animals positive for Babesia spp., but sex and PCV did not. The phylogenetic analysis of sequences showed 4 isolates of B. bovis and 3 isolates of B. bigemina in the 2 study zones, with similarities between 99.73 and 100% with other sequences. One B. bovis isolate was similar in the zone of El Carmen and Quito. Conclusion and significance: This work is the first molecular characterization of B. bigemina and B. bovis in Ecuador, and it is also the first evidence of Babesia spp. in cattle in the area of Quito at an altitude of 2469 m.a.s.l., being the highest altitude reported for animals with babesiosis and for the tick R. microplus. Climatic factors as well as mobility of tick-carrying animals without any control allow the presence of Babesiosis outbreaks in new geographical areas.

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Section: Discussionmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

Detection of Babesia spp. in High Altitude Cattle in Ecuador, Possible Evidence of the Adaptation of Vectors and Diseases to New Climatic Conditions

et al. 2021

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“…was identified in hard ticks (Amblyomma and Rhipicephalus sp.) in Ethiopia [81,82], Mali [18], Côte d'Ivoire [60], Egypt [17], Madagascar [83] and Ecuador [77]. In the present study, a new potential B. theileri was identified in two Rh.…”

Section: Discussionmentioning

confidence: 49%

“…B. theileri in Rh. microplus has been reported in Madagascar [83], Ecuador [77], Brazil [84] and Argentina [85]. Thus, this is the first time that B. theileri has been detected in Rh.…”

Section: Discussionmentioning

confidence: 59%

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Molecular Characterization of Some Equine Vector-Borne Pathogens and Identification of Their Vectors in Egypt

Abdullah¹,

Aboelsoued²,

Farag³

et al. 2020

Preprint

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Background Equine vector borne diseases (EVBD) have been considered emerging and reemerging diseases transmitted by arthropods and most of these diseases have zoonotic concern. This study was designed to screen EVBD in equines and their vectors using molecular analyses and identify vectors by MALDI-TOF and molecular techniques.Methods A total of 335 blood samples were collected from apparently healthy equines (320 from horses and 15 from donkeys) from Cairo and Beni-Suef provinces in Egypt. A total of 166 arthropods (105 sucking flies and 61 ticks) were collected from the same animals. MALDI-TOF and molecular techniques were used to confirm the findings of morphological identification of vector. Quantitative PCR and Standard PCR coupled with sequencing were performed in equines and vectors DNA for screening multiple pathogens.Results MALDI-TOF and molecular techniques confirmed that Hippoposca equina (louse fly), Rhipicephalus annulatus (Rh. annulatus) and Rh. microplus ixodid ticks were found. In vectors, we identified Anaplasma marginale (A. marginale; 1.6%), A. platys-like (1.6%) and a new Ehrlichia sp. (4.9%) in Rh. microplus, while Ehrlichia rustica (E. rustica) was found in Rh. microplus and Rh. annulatus. Likewise, Borrelia theileri was identified in Rh. microplus (3.3%). For H. equina, Anaplasma and Borrelia sp. DNA were detected by qPCR only. In equines, A. marginale (0.6%), A. ovis (0.6%) and Theileria ovis (T. ovis; 0.6%) were found in donkeys. In horses, T. equi (1.2%) and a new Theileria sp. Africa (2.7%) were identified.Conclusions For the first time, we reported here the presence of Rh. microplus as a competent tick for Rh. annulatus in Egypt using MALDI-TOF and molecular identification. To the best of our knowledge, we provided the first detection of different pathogens as A. marginale, A. platys-like, E. rustica, new Ehrlichia sp., B. theileri in Rh. microplus, A. marginale, A. ovis and T. ovis in donkeys and a new Theileria sp. Africa in horses in Egypt.

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Molecular detection of rickettsial agents in Amblyomma maculatum ticks (Ixodida: Ixodidae) from Ecuador

Enríquez,

Félix,

Bermúdez

et al. 2024

Vet Res Commun

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Bovine anaplasmosis and tick-borne pathogens in cattle of the Galapagos Islands

Cited by 19 publications

References 43 publications

Detection of Babesia spp. in High Altitude Cattle in Ecuador, Possible Evidence of the Adaptation of Vectors and Diseases to New Climatic Conditions

Detection of Babesia spp. in High Altitude Cattle in Ecuador, Possible Evidence of the Adaptation of Vectors and Diseases to New Climatic Conditions

Molecular Characterization of Some Equine Vector-Borne Pathogens and Identification of Their Vectors in Egypt

Molecular detection of rickettsial agents in Amblyomma maculatum ticks (Ixodida: Ixodidae) from Ecuador

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