Bovine Adenovirus Type 3 E1Bsmall Protein Is Essential for Growth in Bovine Fibroblast Cells

Zhou, Yan; Reddy, P. Seshidhar; Babiuk, Lorne A.; Tikoo, Suresh K.

doi:10.1006/viro.2001.1104

Cited by 9 publications

(4 citation statements)

References 49 publications

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“…FITC-conjugated goat anti-rabbit antibody, FITC-conjugated goat anti-mouse, alkaline phosphatase (AP)-conjugated goat anti-rabbit IgG, alkaline phosphatase (AP)-conjugated goat anti-mouse IgG, TRITC-conjugated goat anti-rabbit antibody and TRITC-conjugated goat anti-mouse were purchased from Jackson Immune Research. Production and characterization of antibodies against BAdV-3 protein VII [21], DNA binding protein (DBP) [22], protein VIII [23] and protein IVa2 [5] have been described previously.…”

Section: Antibodiesmentioning

confidence: 99%

Regions of Bovine Adenovirus-3 Protein VII Involved in Interactions with Viral and Cellular Proteins

Kulanayake,

Dar,

Tikoo

2024

Viruses

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The L 1 region of bovine adenovirus (BAdV)-3 encodes a multifunctional protein named protein VII. Anti-protein VII sera detected a protein of 26 kDa in transfected or BAdV-3-infected cells, which localizes to nucleus and nucleolus of infected/transfected cells. Analysis of mutant protein VII identified four redundant overlapping nuclear/nucleolar localization signals as deletion of all four potential nuclear/nucleolar localization signals localizes protein VII predominantly to the cytoplasm. The nuclear import of protein VII appears to use importin α (α-1), importin-β (β-1) and transportin-3 nuclear transport receptors. In addition, different nuclear transport receptors also require part of protein VII outside nuclear localization sequences for efficient interaction. Proteomic analysis of protein complexes purified from recombinant BAdV-3 expressing protein VII containing Strep Tag II identified potential viral and cellular proteins interacting with protein VII. Here, we confirm that protein VII interacts with IVa2 and protein VIII in BAdV-3-infected cells. Moreover, amino acids 91–101 and 126–137, parts of non-conserved region of protein VII, are required for interaction with IVa2 and protein VIII, respectively.

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Section: Antibodiesmentioning

confidence: 99%

Regions of Bovine Adenovirus-3 Protein VII Involved in Interactions with Viral and Cellular Proteins

Kulanayake,

Dar,

Tikoo

2024

Viruses

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“…Production and characterization of anti-BAdV-3 E1B small 19K protein [14], DNA-binding protein [15], 52K protein [16], and 100K protein [17] specific sera has been previously described. Anti-hexon sera recognizes a protein of 110 kDa in BAdV-3 infected cells [18].…”

Section: Badv-3 Protein-specific Antibodiesmentioning

confidence: 99%

Bovine Adenovirus-3 Tropism for Bovine Leukocyte Sub-Populations

Khosa

Araya

Griebel

et al. 2020

Viruses

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A number of characteristics including lack of virulence and the ability to grow to high titers, have made bovine adenovirus-3 (BAdV-3) a vector of choice for further development as a vaccine-delivery vehicle for cattle. Despite the importance of blood leukocytes, including dendritic cells (DC), in the induction of protective immune responses, little is known about the interaction between BAdV-3 and bovine blood leukocytes. Here, we demonstrate that compared to other leukocytes, bovine blood monocytes and neutrophils are significantly transduced by BAdV404a (BAdV-3, expressing enhanced yellow green fluorescent protein [EYFP]) at a MOI of 1–5 without a significant difference in the mean fluorescence of EYFP expression. Moreover, though expression of some BAdV-3-specific proteins was observed, no progeny virions were detected in the transduced monocytes or neutrophils. Interestingly, addition of the “RGD” motif at the C-terminus of BAdV-3 minor capsid protein pIX (BAV888) enhanced the ability of the virus to enter the monocytes without altering the tropism of BAdV-3. The increased uptake of BAV888 by monocytes was associated with a significant increase in viral genome copies and the abundance of EYFP and BAdV-3 19K transcripts compared to BAdV404a-transduced monocytes. Our results suggest that BAdV-3 efficiently transduces monocytes and neutrophils in the absence of viral replication. Moreover, RGD-modified capsid significantly increases vector uptake without affecting the initial interaction with monocytes.

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“…Isolation of replication competent BAdV-3 containing partial deletion of E1B small suggested that this region is not essential for replication of BAdV-3 [51] in fetal bovine retina cells and may be used for foreign gene insertion. However, viable recombinant BAdV-3 with E1A [52][53][54] or E1B large deletion [33,52] could not be rescued suggesting that these regions are essential for replication of BAdV-3.…”

Section: E1 Regionmentioning

confidence: 99%

Bovine adenovirus-3 as a vaccine delivery vehicle

Ayalew

Kumar

Gaba

et al. 2015

Vaccine

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The use of vaccines is an effective and relatively inexpensive means of controlling infectious diseases, which cause heavy economic losses to the livestock industry through animal loss, decreased productivity, treatment expenses and decreased carcass quality. However, some vaccines produced by conventional means are imperfect in many respects including virulence, safety and efficacy. Moreover, there are no vaccines for some animal diseases. Although genetic engineering has provided new ways of producing effective vaccines, the cost of production for veterinary use is a critical criterion for selecting the method of production and delivery of vaccines. The cost effective production and intrinsic ability to enter cells has made adenovirus vectors a highly efficient tool for delivery of vaccine antigens. Moreover, adenoviruses induce both humoral and cellular immune responses to expressed vaccine antigens. Since nonhuman adenoviruses are species specific, the development of animal specific adenoviruses as vaccine delivery vectors is being evaluated. This review summarizes the work related to the development of bovine adenovirus-3 as a vaccine delivery vehicle in animals, particularly cattle.

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Bovine Adenovirus Type 3 E1Bsmall Protein Is Essential for Growth in Bovine Fibroblast Cells

Cited by 9 publications

References 49 publications

Regions of Bovine Adenovirus-3 Protein VII Involved in Interactions with Viral and Cellular Proteins

Regions of Bovine Adenovirus-3 Protein VII Involved in Interactions with Viral and Cellular Proteins

Bovine Adenovirus-3 Tropism for Bovine Leukocyte Sub-Populations

Bovine adenovirus-3 as a vaccine delivery vehicle

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