Vessel-wall measurements from multicontrast MRI provide information on plaque structure and evolution. This requires the extraction of numerous contours. In this work a contour-extraction method is proposed that uses an active contour model (NLSnake) adapted for a wide range of MR vascular images. This new method employs length normalization for the purpose of deformation computation and offers the advantages of simplified parameter tuning, fast convergence, and minimal user interaction. The model can be initialized far from the boundaries of the region to be segmented, even by only one pixel. The accuracy and reproducibility of NLSnake endoluminal contours were assessed on vascular phantom MR angiography (MRA) and high-resolution in vitro MR images of rabbit aorta. An in vivo evaluation was performed on rabbit and clinical data for both internal and external vessel-wall contours. In phantoms with 95% stenoses, NLSnake measured 94.3% ؎ 3.8%, and the accuracy was even better for milder stenoses. In the images of rabbit aorta, variability between NLSnake and experts was less than interobserver variability, while the maximum intravariability of NLSnake was equal to 1.25%. In conclusion, the NLSnake technique successfully quantified the vessel lumen in multicontrast MR images using constant parameters. The determination of lumen narrowing by an angiographic technique is still the reference measurement for evaluating atherosclerosis. However, measurements in the vessel wall would be more predictive. High-resolution MRI has emerged as a powerful noninvasive technique for the evaluation of vessel-wall abnormalities. Its usefulness for the in vitro and in vivo study of plaque evolution has been demonstrated in humans and in animal models (1-7). The use of multicontrast MRI with measurements of the lumen and outer vessel-wall circumference, area, and thickness, and the contrast-to-noise ratio (CNR) and signal-to-noise ratio (SNR) in the vessel wall provides information on plaque structure and evolution (8,9). These measurements are generally based on a manual extraction of numerous contours, which requires at least two medical experts to preserve objectivity. Thus, with the increasing number of MRI exams, a fast, automated postprocessing algorithm is necessary for multicenter longitudinal studies. However, there are several difficulties related to the characteristics of the MR data. Acquisitions are performed in a multicontrast mode (such as MR angiography (MRA), high-resolution T 1 , T 2 , and proton density), with the lumen blood appearing black or white. SNR and CNR in the surrounding tissues are highly dependent on the acquisition parameters (e.g., spatial resolution and fat saturation) and the vessel-wall composition. Vessel-wall signal is often heterogeneous in pathologic cases. Thus, it is difficult to detect inner and outer contours automatically. Moreover, minimal user-interaction is required, and the process should be fast and reproducible.In this study we describe a method based on an activecontour model adapt...