Botulinum C3 Enzyme Changes the Lactate Dehydrogenase Isozyme Pattern of Primary Culture of Neurons.

Abstract

ABSTRACT. Changes in the lactate dehydrogenase (LDH) isozyme pattern of primary culture of neurons treated with botulinum C3 enzyme were examined in order to elucidate the functional changes accompanying the morphological change that follows ADP-ribosylation of Rho protein. Primary neurons were prepared from the cerebrum of ICR mouse embryos on day 15. Neurons were cultured in MEM with 10% fetal calf serum at 37°C. In the neurons treated with C3 enzyme, a typical morphological change was observed after 24 hr, and the LDH isozyme pattern was changed after 72 hr. The ratio of H-subunit to M-subunit in LDH was decreased by C3 treatment, suggesting the induction of a state of lower intracellular oxygen consumption in neurons in the primary cultures.-KEY WORDS: botulinum C3, C3 enzyme, LDH isozyme pattern, mouse neuron, primary culture.

The Evaluation of the Potential of Botulinum C3 Enzyme as an Exogenous Differentiation Inducing Factor to Neurons.

“…Then we examined the change of a metabolic enzyme, especially LDH isozyme. The LDH isozyme pattern of C3-treated neurons was changed to a reduced oxygen consumption type [21]. This type of change is considered to have an advantage over oxygen deficiency in ischemic neural disorder.…”

“…Botulinum C3 enzyme produced by Clostridium botulinum type C and D strains modifies Rho proteins. In a previous study, we observed that the LDH isozyme pattern of neurons treated with C3 enzyme was different from that induced with endogenous growth factor of neurons such as NGF [21]. This type of change is considered to have an advantage in the medical use of C3 enzyme for neural disorder.…”

“…Also, the ratio of H/M in LDH was increased by nerve growth factor (NGF) and N6, 2'-O-dibutyryl 3', 5'-cyclic monophosphate (DBcAMP) [21]. These results suggest that the C3 enzyme induces an intracellular state in which less oxygen is consumed in primary cultured neurons.…”