Bottom‐up effect of host plants on life‐history characteristics of <i>Aphidoletes aphidimyza</i> feeding on <i>Aphis gossypii</i>

Madahi, Khadijeh; Sahragard, Ahad; Hosseini, Reza; Baniameri, Valiollah

doi:10.1111/eea.12824

Cited by 4 publications

(1 citation statement)

References 67 publications

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“…The Aphidoletes aphidimyza Rondani (Diptera: Cecidomyiidae) is widely utilized as an efficient predator of aphids in various agricultural systems, and its larvae can prey voraciously on more than 80 aphid species (Boulanger et al, 2019). To date studies on A. aphidimyza have focused on biological characteristics (Choi et al, 2004; OPEN ACCESS EDITED BY Zhijun Yu, Hebei Normal University, China Guo et al, 2014;Watanabe et al, 2016;De Azevedo et al, 2018;Madahi et al, 2019;Fratoni et al, 2020;Higashida et al, 2022), biological control (Lin et al, 2017;Shang et al, 2019), and the mitochondrial genome (Shen et al, 2022), but little is known about molecular mechanisms. Gene expression-level analysis is fundamental in the study of regulatory mechanisms of genes related to host selection, predation, drug resistance, stagnation, and neuromodulation.…”

Section: Introductionmentioning

confidence: 99%

Selection and validation of optimal reference genes for RT-qPCR analyses in Aphidoletes aphidimyza Rondani (Diptera: Cecidomyiidae)

Shen,

Zhang,

Zhao

et al. 2023

Front. Physiol.

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Aphidoletes aphidimyza is a predator that is an important biological agent used to control agricultural and forestry aphids. Although many studies have investigated its biological and ecological characteristics, few molecular studies have been reported. The current study was performed to identify suitable reference genes to facilitate future gene expression and function analyses via quantitative reverse transcription PCR. Eight reference genes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), RPS13, RPL8, RPS3, α-Tub, β-actin, RPL32, and elongation factor 1 alpha (EF1-α) were selected. Their expression levels were determined under four different experimental conditions (developmental stages, adult tissues, sugar treatment, and starvation treatment) using qRT-PCR technology. The stability was evaluated with five methods (Ct value, geNorm, NormFinder, BestKeeper, and RefFinder). The results showed that GAPDH, RPL32, and EF1-α were ranked as the best reference gene combinations for measuring gene expression levels among different developing stages and in various starvation treatments. RPL8 and RPS3 were recommended to normalize the gene expression levels among different adult tissues. RPL32, β-actin, and EF1-α were recommended sugar-feeding conditions. To validate the utility of the selected reference pair, RPL8, and RPS3, we estimated the tissue-biased expression level of a chemosensory protein gene (AaphCSP1). As expected, AaphCSP1 is highly expressed in the antennae and lowly expressed in the abdomen. These findings will lay the foundation for future research on the molecular physiology and biochemistry of A. aphidimyza.

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