Bottom-Up Analysis of Proteins by Peptide Mass Fingerprinting with tCITP-CZE-ESI-TOF MS After Tryptic Digest

Segl, Marius; Stutz, Hanno

doi:10.1007/978-1-0716-2493-7_7

Cited by 1 publication

(2 citation statements)

References 22 publications

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“…Moreover, they were complementary, as 758 peptides identified in supernatant samples were common to both protocols, and additional 346 and 226 peptides were unique to acetone purification and acetic acid purification, respectively [115]. In another example, the transient capillary isotachophoresis has been used, first for concentration of tryptic peptides from bovine β‐LG A [116] and then followed by their separation by CZE using acidic BGE. Online coupling of CZE to TOF‐MS via triple‐tube coaxial sheath flow interface with ESI allowed to obtain peptide mass fingerprint and identification [116].…”

Section: Recent Advances In Separation Of Milk Bioactive Proteins/pep...mentioning

confidence: 99%

“…In another example, the transient capillary isotachophoresis has been used, first for concentration of tryptic peptides from bovine β‐LG A [116] and then followed by their separation by CZE using acidic BGE. Online coupling of CZE to TOF‐MS via triple‐tube coaxial sheath flow interface with ESI allowed to obtain peptide mass fingerprint and identification [116]. As well, MS‐based proteomics has been used for quantitative the characterization of differentially expressed MFGM proteins in bovine and donkey colostrum [49].…”

Section: Recent Advances In Separation Of Milk Bioactive Proteins/pep...mentioning

confidence: 99%

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Advances in separation and identification of biologically important milk proteins and peptides

Št́astná

2023

Electrophoresis

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Milk is a rich source of biologically important proteins and peptides. In addition, milk contains a variety of extracellular vesicles (EVs), including exosomes, that carry their own proteome cargo. EVs are essential for cell–cell communication and modulation of biological processes. They act as nature carriers of bioactive proteins/peptides in targeted delivery during various physiological and pathological conditions. Identification of the proteins and protein‐derived peptides in milk and EVs and recognition of their biological activities and functions had a tremendous impact on food industry, medicine research, and clinical applications. Advanced separation methods, mass spectrometry (MS)‐based proteomic approaches and innovative biostatistical procedures allowed for characterization of milk protein isoforms, genetic/splice variants, posttranslational modifications and their key roles, and contributed to novel discoveries. This review article discusses recently published developments in separation and identification of bioactive proteins/peptides from milk and milk EVs, including MS‐based proteomic approaches.

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Section: Recent Advances In Separation Of Milk Bioactive Proteins/pep...mentioning

confidence: 99%

Section: Recent Advances In Separation Of Milk Bioactive Proteins/pep...mentioning

confidence: 99%

Advances in separation and identification of biologically important milk proteins and peptides

Št́astná

2023

Electrophoresis

View full text Add to dashboard Cite

show abstract

Bottom-Up Analysis of Proteins by Peptide Mass Fingerprinting with tCITP-CZE-ESI-TOF MS After Tryptic Digest

Cited by 1 publication

References 22 publications

Advances in separation and identification of biologically important milk proteins and peptides

Advances in separation and identification of biologically important milk proteins and peptides

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