Botrytis cinerea infection affects wax composition, content and gene expression in blueberry fruit

Bo, Jiang; Liu, Ruiling; Fang, Xiangjun; Wu, Wei; Han, Yanchao; Chen, Hangjun; Xing, Feng; Gao, Haiyan

doi:10.1016/j.postharvbio.2022.112020

Cited by 23 publications

(7 citation statements)

References 40 publications

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“…Since lipase is one of Botrytis cinerea's extracellular secretions, and B. cinerea could degrade wax (Jiang, Liu, Fang, Wu, et al, 2022), lipase may have a similar effect, which could down-regulate expression of wax biosynthesis and transport-related genes. PAL, CHI, DFR, UFGT, β-GAL, PPO, and POD are pivotal enzymes in both the biosynthesis and degradation pathways of anthocyanin (Liu et al, 2018b(Liu et al, , 2021Zorenc et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…SEM was used to observe the morphological structure of blueberries by Jiang, Liu, Fang, Wu, et al (2022). Fruits without mechanical damage or cuticular damage were selected, and each section (1 cm in length, 1 cm in width, and 1 mm in depth) was cut with a blade at the equatorial part of the fruit and placed on a slide.…”

Section: Scanning Electron Microscopy Observation Of Cuticular Waxmentioning

confidence: 99%

“…The method referred to Jiang, Liu, Fang, Wu, et al (2022) with slightly modified, 30 complete blueberries were selected from the control group and the lipase treatment group, respectively, in a triangular flask, adding 30 mL chloroform, soaking at room temperature for 1 min, repeated twice. The extract was combined and filtered with quantitative filter paper, and 100 μL (1 g L −1 ) n-tetracosane was added as an internal standard.…”

Section: Cuticular Wax Extraction and Gc-ms Analysismentioning

confidence: 99%

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Impact of lipase treatment on cuticle wax structure and anthocyanin metabolism in postharvest blueberries

Ding,

Zong,

Han

et al. 2024

Food Frontiers

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In this paper, we explored the influence of lipase on cuticle wax and anthocyanin metabolism in blueberries. The results demonstrated a progressive alteration in wax crystal structure, transitions from rod‐like to short rod‐like and flaky, and even disappearance of the cuticle wax. Gas chromatography–mass spectrometry analysis revealed a reduction in esters, aldehydes, long‐chain alkanes, and total wax content following lipase treatment of blueberry fruits. Additionally, ultra high performance liquid chromatography–mass spectrometry analysis showed significant decreases in peonidin, petunidin, cyanidin, and delphinidin, accompanied by an increase in malvidin content due to lipase treatment. Subsequent investigations uncovered that lipase treatment inhibited the activities of key anthocyanin synthetic enzymes, namely phenylalanine ammonia‐lyase (PAL), chalcone isomerase (CHI), and dihydroflavonol reductase (DFR). Moreover, lipase treatment downregulated the expression of synthesis‐related genes, including PAL, 4‐coumarate: CoA ligase, chalcone synthase (CHS), CHI, and DFR. In summary, our findings indicate that lipase disrupts the integrity of cuticle wax structure, inhibits anthocyanin synthesis, and reduces anthocyanin content. Accordingly, our studies suggest that inhibiting lipase could offer a novel approach to preserving the freshness and anthocyanin concentration of blueberries.

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Section: Discussionmentioning

confidence: 99%

Section: Scanning Electron Microscopy Observation Of Cuticular Waxmentioning

confidence: 99%

Section: Cuticular Wax Extraction and Gc-ms Analysismentioning

confidence: 99%

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Impact of lipase treatment on cuticle wax structure and anthocyanin metabolism in postharvest blueberries

Ding,

Zong,

Han

et al. 2024

Food Frontiers

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“…However, blueberries have a limited shelf life of 3–7 days at room temperature, resulting in rapid deterioration and short retail distance ( 7 , 8 ). Furthermore, blueberries with thin skin and higher flesh content are at increased risk of microbial infection ( 9 ). For these reasons, commercial harvest enterprises can incur serious losses from spoilage following a typical summer harvest.…”

Section: Introductionmentioning

confidence: 99%

Analysis of sucrose addition on the physicochemical properties of blueberry wine in the main fermentation

Liu

Wang²,

Weng³

et al. 2023

Front. Nutr.

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IntroductionHarvested blueberries can be processed into wine to extend their shelf life and increase their commercial value. In order to produce fruit wine, external sugar is often added prior to fermentation to increase the final alcohol content to a target of 8–12% (v/v) to meet consumer expectations.Methodwe explore the effect of 8–14% (w/w) sucrose on the physicochemical properties of blueberry wine throughout the main fermentation process. We monitor changes of alcohol content, sugar, color, phenol, acidity, anthocyanin, and odor.Results and discussionWe notice that sucrose affects the fermentation process and physicochemical composition of the final blueberry wine by fermentation rate, fermentation color and protection of functional substances protection. Additional sucrose extends the total time of fermentation, and increases wine acidity. The color of the wine is also affected, with added sugar darkening and yellowing the final product. Interestingly, the sucrose has a protective effect on anthocyanin levels, although total anthocyanin levels are still substantially reduced following fermentation. Finally, the additional sugar increases accumulation of volatile odor components, particularly alcohols and esters, as measured by an electronic nose. We conclude that an addition of 12% sucrose produces wine with superior physicochemical properties of alcohol, anthocyanin loss and odor relative to other conditions tested and recommend this approach to commercial manufacturers.

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“…Plant diseases are one of the important problems facing crop protection at present, and the several typical plant pathogens belonging to oomycetes, ascomycete, deuteromycetes, and basidiomycetes are the main source of crop infections. − Among many plant diseases, gray mold is a serious fungal disease that harms solanaceous vegetables, melon vegetables, leek, lettuce, and so on, especially under the condition of facilities, which is always caused by Botrytis cinerea of fruits and vegetables and occurs in most countries in the world. − This pathogen has a wide host range and fast reproduction and mainly damages the leaves, flower heads, and fruits (stalks), which can reduce the yield by about 40% in serious cases. So, in order to control this disease, different fungicides have been developed and used, however, continuous use of the fungicides is easy to produce resistance. − Conventional chemical fungicides, such as Iprodione, pyrimethanil, and procymidone, have developed significant resistance to varying degrees. − Moreover, there was a positive cross resistance between iprodione and procymidone.…”

Section: Introductionmentioning

confidence: 99%

Discovery of Novel Carboxamide Derivatives Containing Biphenyl Pharmacophore as Potential Fungicidal Agents Used for Resistance Management

Ke,

Gao,

Zhang

et al. 2023

J. Agric. Food Chem.

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Natural products are one of the main sources of drug and agrochemicals discovery. Biphenyls skeleton are ubiquitous structures in many classes of natural products, which indicate extensive biological activities. So, in order to investigate the potential applications for natural biphenyl derivatives, a series of novel carboxamide derivatives with diverse substituent patterns were designed and synthesized based on active pharmacophore from natural biphenyl lignans, and their in vitro antifungal activities against several typical plant pathogens belonging to oomycetes, ascomycete, deuteromycetes, and basidiomycetes were fully investigated. The highly potential compounds were further tested in vivo assay against Botrytis cinerea Pers. of cucumber to demonstrate a practical application for controlling common plant diseases, which indicated four compounds could effectively control the resistant strains of carbendazim, rutamycin, and pyrazolidide. The potential modes of action for compound B12 against B. cinerea were also explored using molecular docking, microscopic technology, and label-free quantitative proteomics analysis. The results show that compound B12 may be a potential novel fungicidal agent used for gray mold resistance control, which can influence the protein synthesis of B. cinerea. These findings can provide a certain theoretical basis for the development of novel biphenyl derivatives as potential green antifungal agents.

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Botrytis cinerea infection affects wax composition, content and gene expression in blueberry fruit

Cited by 23 publications

References 40 publications

Impact of lipase treatment on cuticle wax structure and anthocyanin metabolism in postharvest blueberries

Impact of lipase treatment on cuticle wax structure and anthocyanin metabolism in postharvest blueberries

Analysis of sucrose addition on the physicochemical properties of blueberry wine in the main fermentation

Discovery of Novel Carboxamide Derivatives Containing Biphenyl Pharmacophore as Potential Fungicidal Agents Used for Resistance Management

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