Botrytis cinerea endo-ß-1,4-glucanase Cel5A is expressed during infection but is not required for pathogenesis

Espino, José J.; Brito, Nélida; Noda, Judith; González, Celedonio

doi:10.1016/j.pmpp.2005.06.005

Cited by 52 publications

(44 citation statements)

References 43 publications

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“…This approach has been recently postulated as useful for the identification of putative pathogenicity factors in Botrytis cinerea [19,20]. As reported above, the analysis of the corresponding sets of 2-DE gels revealed an average of 550 protein spots in the proteome of D. seriata .…”

Section: Resultsmentioning

confidence: 87%

“…Since cellulose is one of the major components of the plant cell wall, proteins overexpressed during growth on CMC could be expected to play a significant role in the pathogenicity process. In fact, this strategy has been used to identify putative pathogenicity factors in the phytopathogenic fungus Botrytis cinerea [19,20]. We decided to try this strategy since D. seriata is an endophytic fungi that exerts its pathogenicity by developing inside the vascular system of grapevine, and unfortunately to date no experimental approach is available that mimics the natural conditions in which D. seriata is developing its pathogenicity.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: a phytopathogenic fungus involved in grapevine decline

et al. 2010

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BackgroundThe phytopathogenic fungus Diplodia seriata, whose genome remains unsequenced, produces severe infections in fruit trees (fruit blight) and grapevines. In this crop is recognized as one of the most prominent pathogens involved in grapevine trunk disease (or grapevine decline). This pathology can result in the death of adult plants and therefore it produces severe economical losses all around the world. To date no genes or proteins have been characterized in D. seriata that are involved in the pathogenicity process. In an effort to help identify potential gene products associated with pathogenicity and to gain a better understanding of the biology of D. seriata, we initiated a proteome-level study of the fungal mycelia and secretome.ResultsIntracellular and secreted proteins from D. seriata collected from liquid cultures were separated using two-dimensional gel electrophoresis. About 550 cytoplasmic proteins were reproducibly present in 3 independent extractions, being 53 identified by peptide mass fingerprinting and tandem mass spectrometry. The secretome analysis showed 75 secreted proteins reproducibly present in 3 biological replicates, being 16 identified. Several of the proteins had been previously identified as virulence factors in other fungal strains, although their contribution to pathogenicity in D. seriata remained to be analyzed. When D. seriata was grown in a medium supplemented with carboxymethylcellulose, 3 proteins were up-regulated and 30 down-regulated. Within the up-regulated proteins, two were identified as alcohol dehydrogenase and mitochondrial peroxyrredoxin-1, suggesting that they could play a significant role in the pathogenicity process. As for the 30 down-regulated proteins, 9 were identified being several of them involved in carbohydrate metabolism.ConclusionsThis study is the first report on proteomics on D. seriata. The proteomic data obtained will be important to understand the pathogenicity process. In fact, several of the identified proteins have been reported as pathogenicity factors in other phytopathogenic fungi. Moreover, this proteomic analysis supposes a useful basis for deepening into D. seriata knowledge and will contribute to the development of the molecular biology of this fungal strain as it has been demonstrated by cloning the gene Prx1 encoding mitochondrial peroxiredoxin-1 of D. seriata (the first gene to be cloned in this microorganism; data not shown).

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Section: Resultsmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: a phytopathogenic fungus involved in grapevine decline

et al. 2010

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“…The host CW is a primary target during B. cinerea growth on plant tissue (30). B. cinerea possesses a wide array of CWDEs (31,32), including six PGs (33). B. cinerea mutants of either BcPG1 or BcPG2 resulted in significantly decreased virulence on multiple hosts, including tomato (5,8).…”

Section: Discussionmentioning

confidence: 99%

The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea

Cantu

Vicente²,

Greve³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

265

209

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Fruit ripening is characterized by processes that modify texture and flavor but also by a dramatic increase in susceptibility to necrotrophic pathogens, such as Botrytis cinerea. Disassembly of the major structural polysaccharides of the cell wall (CW) is a significant process associated with ripening and contributes to fruit softening. In tomato, polygalacturonase (PG) and expansin (Exp) are among the CW proteins that cooperatively participate in ripening-associated CW disassembly. To determine whether endogenous CW disassembly influences the ripening-regulated increase in necrotropic pathogen susceptibility, B. cinerea susceptibility was assessed in transgenic fruit with suppressed polygalacturonase (LePG) and expansin (LeExp1) expression. Suppression of either LePG or LeExp1 alone did not reduce susceptibility but simultaneous suppression of both dramatically reduced the susceptibility of ripening fruit to B. cinerea, as measured by fungal biomass accumulation and by macerating lesion development. These results demonstrate that altering endogenous plant CW disassembly during ripening influences the course of infection by B. cinerea, perhaps by changing the structure or the accessibility of CW substrates to pathogen CW-degrading enzymes. Recognition of the role of ripening-associated CW metabolism in postharvest pathogen susceptibility may be useful in the design and development of strategies to limit pathogen losses during fruit storage, handling, and distribution.expansin ͉ polygalacturonase ͉ tomato ͉ plant pathogen

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“…B. cinerea secretes many enzymes and metabolites that are presumed to enable the fungus to kill and subsequently feed on plant cells (van Kan, 2006). B. cinerea enzymes that have been studied in this context are mostly enzymes that can degrade plant cuticle and cell wall components such as cutin (van der VlugtBergmans et al, 1997;van Kan et al, 1997), pectin (Kars et al, 2005a,b;ten Have et al, 1998), hemicellulose (Brito et al, 2006) or cellulose (Espino et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

The Botrytis cinerea aspartic proteinase family

Have¹,

Espino²,

Dekkers³

et al. 2010

Fungal Genetics and Biology

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Botrytis cinerea endo-ß-1,4-glucanase Cel5A is expressed during infection but is not required for pathogenesis

Cited by 52 publications

References 43 publications

Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: a phytopathogenic fungus involved in grapevine decline

Cytoplasmic- and extracellular-proteome analysis of Diplodia seriata: a phytopathogenic fungus involved in grapevine decline

The intersection between cell wall disassembly, ripening, and fruit susceptibility to Botrytis cinerea

The Botrytis cinerea aspartic proteinase family

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