Botrytis cinerea BcNma is involved in apoptotic cell death but not in stress adaptation

Finkelshtein, Alin; Shlezinger, Neta; Bunis, Olga; Sharon, Amir

doi:10.1016/j.fgb.2011.01.007

Cited by 22 publications

(22 citation statements)

References 30 publications

(35 reference statements)

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“…The CA allele of BcRAC (G17V) was generated by site-directed mutagenesis of pTZHABcRAC, using primers 24 and 25, resulting in pTZHA-CA-BcRAC. The HA-tagged CA-bcrac fragment was excised from pTZHA-CA-BcRAC with PacI and AscI and introduced under the control of the B. cinerea actin promoter into the vector KSHANG (29), which also carries the hygromycin resistance cassette, to form the pKSH-HA-CA-BcRAC plasmid. Diagnostic PCR of the hygromycin-resistant transformants was carried out with primers 22 (tag specific) and 21 to verify expression of the constitutively active protein.…”

Section: Methodsmentioning

confidence: 99%

Involvement of Botrytis cinerea Small GTPases BcRAS1 and BcRAC in Differentiation, Virulence, and the Cell Cycle

et al. 2013

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bSmall GTPases of the Ras superfamily are highly conserved proteins that are involved in various cellular processes, in particular morphogenesis, differentiation, and polar growth. Here we report on the analysis of RAS1 and RAC homologues from the gray mold fungus Botrytis cinerea. We show that these small GTPases are individually necessary for polar growth, reproduction, and pathogenicity, required for cell cycle progression through mitosis (BcRAC), and may lie upstream of the stress-related mitogenactivated protein kinase (MAPK) signaling pathway. bcras1 and bcrac deletion strains had reduced growth rates, and their hyphae were hyperbranched and deformed. In addition, both strains were vegetatively sterile and nonpathogenic. A strain expressing a constitutively active (CA) allele of the BcRAC protein had partially similar but milder phenotypes. Similar to the deletion strains, the CA-BcRAC strain did not produce any conidia and had swollen hyphae. In contrast to the two deletion strains, however, the growth rate of the CA-BcRAC strain was normal, and it caused delayed but well-developed disease symptoms. Microscopic examination revealed an increased number of nuclei and disturbance of actin localization in the CA-BcRAC strain. Further work with cell cycle-and RAC-specific inhibitory compounds associated the BcRAC protein with progression of the cell cycle through mitosis, possibly via an effect on microtubules. Together, these results show that the multinucleate phenotype of the CA-BcRAC strain could result from at least two defects: disruption of polar growth through disturbed actin localization and uncontrolled nuclear division due to constitutive activity of BcRAC.

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Section: Methodsmentioning

confidence: 99%

Involvement of Botrytis cinerea Small GTPases BcRAS1 and BcRAC in Differentiation, Virulence, and the Cell Cycle

et al. 2013

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“…For tagging of Histone-1, a 1903 bp PCR fragment was amplified (primer pair 12/13) from pMF-280 plasmid (Freitag et al, 2004) with restriction sites EcoRV and AscI. The resulting fragment was cloned into over-expression vector pKS-HAIG (Finkelshtein et al, 2011) at PacI (that was made blunt-ended by treatment with T4 DNA polymerase) and AscI sites. The resulting fusion vector was named pKSH-H1GFP (Shlezinger et al, in press) and used for the transformation of wild type B05.10 and Dbccdc42 strains.…”

Section: Vector Constructionmentioning

confidence: 99%

The small GTPase BcCdc42 affects nuclear division, germination and virulence of the gray mold fungus Botrytis cinerea

Kokkelink

Minz

Al-Masri

et al. 2011

Fungal Genetics and Biology

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“…In contrast, the C-terminal repeat contains an incomplete serine protease site that is supposed to be non-functional. Nma111p is able to promote apoptotic cell death in S. cerevisiae [12], similarly to its recently identified homologue BcNma of the grey mould Botrytis cinerea [13] as well as its closest metazoan homologues, human and Drosophila Omi/HtrA2 [7,[14][15][16][17][18][19]. In addition, Nma111p is implicated in lipid homoeostasis and exhibits chaperone activity [20,21].…”

Section: Introductionmentioning

confidence: 89%

Nma111p, the pro-apoptotic HtrA-like nuclear serine protease in Saccharomyces cerevisiae: a short survey

Fahrenkrog

2011

Biochemical Society Transactions

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The baker's yeast, Saccharomyces cerevisiae, is also capable of undergoing programmed cell death or apoptosis, for example in response to viral infection as well as during chronological and replicative aging. Intrinsically, programmed cell death in yeast can be induced by, for example, H2O2, acetic acid or the mating-type pheromone. A number of evolutionarily conserved apoptosis-regulatory proteins have been identified in yeast, one of which is the HtrA (high-temperature requirement A)-like serine protease Nma111p (Nma is nuclear mediator of apoptosis). Nma111p is a nuclear serine protease of the HtrA family, which targets Bir1p, the only known inhibitor-of-apoptosis protein in yeast. Nma111p mediates apoptosis in a serine-protease-dependent manner and exhibits its activity exclusively in the nucleus. How the activity of Nma111p is regulated has remained largely elusive, but some evidence points to a control by phosphorylation. Current knowledge of Nma111p's function in apoptosis will be discussed in the present review.

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Botrytis cinerea BcNma is involved in apoptotic cell death but not in stress adaptation

Cited by 22 publications

References 30 publications

Involvement of Botrytis cinerea Small GTPases BcRAS1 and BcRAC in Differentiation, Virulence, and the Cell Cycle

Involvement of Botrytis cinerea Small GTPases BcRAS1 and BcRAC in Differentiation, Virulence, and the Cell Cycle

The small GTPase BcCdc42 affects nuclear division, germination and virulence of the gray mold fungus Botrytis cinerea

Nma111p, the pro-apoptotic HtrA-like nuclear serine protease in Saccharomyces cerevisiae: a short survey

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