Botryane terpenoids produced by Nemania bipapillata, an endophytic fungus isolated from red alga Asparagopsis taxiformis - Falkenbergia stage

Medina, Rebeca Previate; Araújo, Ângela R.; Batista, João M.; Cardoso, Carmen L.; Seidl, Claudia; Vilela, Adriana Ferreira Lopes; Domingos, Helori Vanni; Costa-Lotufo, Letı́cia V.; Andersen, Raymond J.; Silva, Dulce Helena Siqueira

doi:10.1038/s41598-019-48655-7

Cited by 30 publications

(22 citation statements)

References 32 publications

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“…isolated from D. nobile was reported to have potential antihyperlipidemic activities [45]. Similarly, botryane sesquiterpenes have been reported from another fungus isolated in the present study Nemania bipapillata by Medina et al [46]. This compound has been shown to have cholinesterase inhibitory activities, which could be useful as lead structures for developing more potent analogs.…”

Section: Discussionsupporting

confidence: 74%

An amalgam of pathogenic and beneficial endophytic fungi colonizing four Dendrobium species from Meghalaya, India

Hajong

Kapoor

2020

J Basic Microbiol

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Endophytic fungi are known to play an important role in driving the evolution of plants by conferring adaptational advantages to their host through the production of secondary metabolites and phytohormones. In this study, we evaluated the diversity and phylogenetic relationship of endophytic fungal communities from four Dendrobium species viz., Dendrobium chrysanthum, Dendrobium heterocarpum, Dendrobium hookerianum, and Dendrobium longicornu of Meghalaya, India. A total of 51 culturable endophytic fungi were isolated from the four selected orchid species. The isolates were identified based on nuclear large subunit sequences into 33 species. Approximately 91% of the isolates showed affinity to Ascomycetes, while 9% of the isolates showed BLAST search similarity to Basidiomycetes. The most common genera were Trichoderma and Xylaria. The most prevalent genera were Fusarium, which was detected in all the four Dendrobium species followed by Diaporthe, which was present in three Dendrobium species viz., D. chrysanthum, D. hookerianum, and D. heterocarpum. The Shannon index value of endophytic fungal communities was the highest in D. chrysanthum (2.66), while D. longicornu (1) had the highest Evenness index. The present study revealed that endophytic fungi in these orchids are an amalgam of pathogenic and beneficial fungi, which have, at the least, switched their lifestyle to asymptomatic endophyte in their host. To our knowledge, this is the first such report on the diversity of endophytic fungi in the four selected Dendrobium species from Meghalaya, India.

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Section: Discussionsupporting

confidence: 74%

An amalgam of pathogenic and beneficial endophytic fungi colonizing four Dendrobium species from Meghalaya, India

Hajong

Kapoor

2020

J Basic Microbiol

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“…Next, postbiotic elements were obtained according to Medina et al ( 2019 ). In total, 4 L of supernatant were extracted with EtOAc (3 × 50% of each medium volume) for the extraction of the active compounds.…”

Section: Methodsmentioning

confidence: 99%

The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris

Rossoni

Barros

Mendonça

et al. 2020

Front. Cell. Infect. Microbiol.

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Candida auris has emerged as a medically important pathogen with considerable resistance to antifungal agents. The ability to produce biofilms is an important pathogenicity feature of this species that aids escape of host immune responses and antimicrobial agents. The objective of this study was to verify antifungal action using in vitro and in vivo models of the Lactobacillus paracasei 28.4 probiotic cells and postbiotic activity of crude extract (LPCE) and fraction 1 (LPF1), derived from L. paracasei 28.4 supernatant. Both live cells and cells free supernatant of L. paracasei 28.4 inhibited C. auris suggesting probiotic and postbiotic effects. The minimum inhibitory concentration (MIC) for LPCE was 15 mg/mL and ranges from 3.75 to 7.5 mg/mL for LPF1. Killing kinetics determined that after 24 h treatment with LPCE or LPF1 there was a complete reduction of viable C. auris cells compared to fluconazole, which decreased the initial inoculum by 1-logCFU during the same time period. LPCE and LPF1 significantly reduced the biomass ( p = 0.0001) and the metabolic activity ( p = 0.0001) of C. auris biofilm. There was also a total reduction (~10 8 CFU/mL) in viability of persister C. auris cells after treatment with postbiotic elements ( p < 0.0001). In an in vivo study, injection of LPCE and LPF1 into G. mellonella larvae infected with C. auris prolonged survival of these insects compared to a control group ( p < 0.05) and elicited immune responses by increasing the number of circulating hemocytes and gene expression of antimicrobial peptide galiomicin. We concluded that the L. paracasei 28.4 cells and postbiotic elements (LPCE and LPF1) have antifungal activity against planktonic cells, biofilms, and persister cells of C. auris . Postbiotic supplementation derived from L. paracasei 28.4 protected G. mellonella infected with C. auris and enhanced its immune status indicating a dual function in modulating a host immune response.

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“…The Konkan coast of India is a relatively unexplored environment for endophytic fungi and their bioactive compounds 18 . Earlier studies have acknowledged marine algicolous endophytic fungi as hot factories of bioactive compounds because of their unique environmental settings 27 . This study has been performed to evaluate the cytotoxic potential of a marine-derived endophytic fungus and to explore its unique productome which can either be novel or have the capacity to replace the existing sources of the natural product.…”

Section: Discussionmentioning

confidence: 99%

Endophytic fungus, Chaetomium globosum, associated with marine green alga, a new source of Chrysin

Kamat

Kumari

Sajna

et al. 2020

Sci Rep

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The marine ecosystem is an extraordinary reserve of pharmaceutically important, bioactive compounds even in this “synthetic age”. Marine algae-associated endophytic fungi have gained prominence as an important source of bioactive compounds. This study was conducted on secondary metabolites of Chaetomium globosum-associated with marine green alga Chaetomorpha media from the Konkan coastline, India. Its ethyl acetate extract (CGEE) exhibited an IC50 value of 7.9 ± 0.1 µg/mL on MCF-7 cells. CGEE exhibited G2M phase cell cycle arrest, ROS production and MMP loss in MCF-7 cells. The myco-components in CGEE contributing to the cytotoxicity were found by Gas Chromatography/Mass Spectrometry analyses. Chrysin, a dihydroxyflavone was one of the forty-six myco-components which is commonly found in honey, propolis and passionflower extracts. The compound was isolated and characterized as fungal chrysin using HPLC, UV–Vis spectroscopy, LC–MS, IR and NMR analyses by comparing with standard chrysin. The purified compound exhibited an IC50 value of 49.0 ± 0.6 µM while that of standard chrysin was 48.5 ± 1.6 µM in MCF-7 cells. It induced apoptosis, G1 phase cell cycle arrest, MMP loss, and ROS production. This is the first report of chrysin from an alternative source with opportunities for yield enhancement.

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Botryane terpenoids produced by Nemania bipapillata, an endophytic fungus isolated from red alga Asparagopsis taxiformis - Falkenbergia stage

Cited by 30 publications

References 32 publications

An amalgam of pathogenic and beneficial endophytic fungi colonizing four Dendrobium species from Meghalaya, India

An amalgam of pathogenic and beneficial endophytic fungi colonizing four Dendrobium species from Meghalaya, India

The Postbiotic Activity of Lactobacillus paracasei 28.4 Against Candida auris

Endophytic fungus, Chaetomium globosum, associated with marine green alga, a new source of Chrysin

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