Bothrops pauloensis snake venom-derived Asp-49 and Lys-49 phospholipases A2 mediates acute kidney injury by oxidative stress and release of inflammatory cytokines

Silveira, João Alison de Moraes; Filho, Adriano José Maia Chaves; Jorge, Antônio Rafael Coelho; Júnior, Francisco Assis Nogueira; Pereira, Venúcia Bruna Magalhães; Aquino, Pedro Éverson Alexandre de; Pereira, Cássia Arruda Souza; Evangelista, Janaína Serra Azul Monteiro; Macêdo, Danielle Silveira; Júnior, Roberto César Pereira Lima; Toyama, Marcos H.; Jorge, Roberta Jeane Bezerra; Pereira, Gustavo José Silva; Monteiro, Helena Serra Azul

doi:10.1016/j.toxicon.2020.12.004

Cited by 13 publications

(9 citation statements)

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“…Following muscle injury, an acute inflammatory reaction is initiated, a process that requires the infiltration of inflammatory cells, the release of growth factors, and pro-or anti-inflammatory cytokines [12]. Numerous studies have shown that Bothropic venoms induce the release of pro-inflammatory cytokines at the site of venom injection, thereby contributing to the exacerbation of local tissue damage [37][38][39][40]. In this study, we assessed the impact of PBM on the release of pro-and anti-inflammatory cytokines IL-6, IL-1β, TNF-α by C2C12 cells following incubation with venom.…”

Section: Discussionmentioning

confidence: 99%

Photobiomodulation mitigates Bothrops jararacussu venom-induced damage in myoblast cells by enhancing myogenic factors and reducing cytokine production

Silva,

Gouveia,

Campos

et al. 2024

PLoS Negl Trop Dis

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Background Photobiomodulation has exhibited promise in mitigating the local effects induced by Bothrops snakebite envenoming; however, the mechanisms underlying this protection are not yet fully understood. Herein, the effectiveness of photobiomodulation effects on regenerative response of C2C12 myoblast cells following exposure to Bothrops jararacussu venom (BjsuV), as well as the mechanisms involved was investigated. Methodology/Principal findings C2C12 myoblast cells were exposed to BjsuV (12.5 μg/mL) and irradiated once for 10 seconds with laser light of 660 nm (14.08 mW; 0.04 cm2; 352 mW/cm2) or 780 nm (17.6 mW; 0.04 cm2; 440 mW/ cm2) to provide energy densities of 3.52 and 4.4 J/cm2, and total energies of 0.1408 and 0.176 J, respectively. Cell migration was assessed through a wound-healing assay. The expression of MAPK p38-α, NF-Кβ, Myf5, Pax-7, MyoD, and myogenin proteins were assessed by western blotting analysis. In addition, interleukin IL1-β, IL-6, TNF-alfa and IL-10 levels were measured in the supernatant by ELISA. The PBM applied to C2C12 cells exposed to BjsuV promoted cell migration, increase the expression of myogenic factors (Pax7, MyF5, MyoD and myogenin), reduced the levels of proinflammatory cytokines, IL1-β, IL-6, TNF-alfa, and increased the levels of anti-inflammatory cytokine IL-10. In addition, PBM downregulates the expression of NF-kB, and had no effect on p38 MAKP. Conclusion/Significance These data demonstrated that protection of the muscle cell by PBM seems to be related to the increase of myogenic factors as well as the modulation of inflammatory mediators. PBM therapy may offer a new therapeutic strategy to address the local effects of snakebite envenoming by promoting muscle regeneration and reducing the inflammatory process.

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Section: Discussionmentioning

confidence: 99%

Photobiomodulation mitigates Bothrops jararacussu venom-induced damage in myoblast cells by enhancing myogenic factors and reducing cytokine production

Silva,

Gouveia,

Campos

et al. 2024

PLoS Negl Trop Dis

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“…However, it is known that kidney injuries can be produced by the isolated or combined action of different ischemic and/or nephrotoxic mechanisms triggered by the biological activities of venoms in the body [8]. Studies with isolated kidneys have shown that venoms from different Bothrops species alter renal function parameters that vary according to the venom composition [8,[61][62][63].…”

Section: Systemic Effectsmentioning

confidence: 99%

“…Bothrops venom toxins are also capable of inducing cytokine synthesis. Cytokine release in the kidney increased by the action of Asp-49 PLA 2 (IL-10) and Lys-49 PLA 2 (TNF-α, IL-1β, IL-10) from B. pauloensis venom [62], while levels of TNF-α, IL-1β, and IL-10 increased in isolated kidneys perfused with B. alternatus venom [124]. BJ-PLA 2 -I, a PLA 2 Asp49 from B. jararaca venom, was found to induce increases in IL-6 and IL-1β in the inflammatory exudate of mice [125].…”

Section: Cytokines and Their Role In Inflammation: An Overviewmentioning

confidence: 99%

Crosstalk of Inflammation and Coagulation in Bothrops Snakebite Envenoming: Endogenous Signaling Pathways and Pathophysiology

Cavalcante

Almeida

Santos-Filho

et al. 2023

IJMS

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Snakebite envenoming represents a major health problem in tropical and subtropical countries. Considering the elevated number of accidents and high morbidity and mortality rates, the World Health Organization reclassified this disease to category A of neglected diseases. In Latin America, Bothrops genus snakes are mainly responsible for snakebites in humans, whose pathophysiology is characterized by local and systemic inflammatory and degradative processes, triggering prothrombotic and hemorrhagic events, which lead to various complications, organ damage, tissue loss, amputations, and death. The activation of the multicellular blood system, hemostatic alterations, and activation of the inflammatory response are all well-documented in Bothrops envenomings. However, the interface between inflammation and coagulation is still a neglected issue in the toxinology field. Thromboinflammatory pathways can play a significant role in some of the major complications of snakebite envenoming, such as stroke, venous thromboembolism, and acute kidney injury. In addition to exacerbating inflammation and cell interactions that trigger vaso-occlusion, ischemia–reperfusion processes, and, eventually, organic damage and necrosis. In this review, we discuss the role of inflammatory pathways in modulating coagulation and inducing platelet and leukocyte activation, as well as the inflammatory production mediators and induction of innate immune responses, among other mechanisms that are altered by Bothrops venoms.

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“…It has been established that their toxic substances exhibit a wide range of pathological effects in relation to most vital systems, causing damage to the lungs, heart, kidneys, skeletal muscles, etc. [6,11,12,14]. However, the number of experimental works on the influence of the venom of various species of snakes and vipers on the morpho-functional changes of the liver is too limited.…”

Section: Introductionmentioning

confidence: 99%

Histological changes in the liver of rats under the influence of Vipera berus berus venom

Turbal,

Yaremenko,

Maievskyi

2023

Rep. of Morph.

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Animal venoms contain a whole complex of compounds, among which there are peptides, proteins, as well as other organic molecules and salts. Their toxins are able to cause pronounced disturbances in the functioning of physiological systems, leading to the appearance of pathological conditions, complications, or even death. The aim of the research is to study the histological changes in the liver of rats under the influence of Vipera berus berus venom. Experimental studies were carried out on white non-linear male rats. The animals were conditionally divided into two groups – a control and an experimental group of 10 individuals in each. Experimental rats were injected intraperitoneally with a semi-lethal dose (LD50) (1.576 mg/g-1) of Vipera berus berus venom in saline solution. Animals of the control group were injected intraperitoneally with only saline solution. Rats were removed from the experiment 24 hours after exposure to the venom, anesthetized by cervical dislocation. Liver samples of animals of all groups were taken for microscopic examination. Histological preparations of the liver were stained with hematoxylin and eosin. Histological preparations were studied using a SEO SСAN light microscope. To identify the key enzyme of the citric acid cycle – succinate dehydrogenase, histochemical studies were performed according to the Nakhlas method. To study the features of glycogen accumulation in hepatocytes, sections were stained using Schiff's reagent, after pretreatment with iodic acid (PAS reaction) in Shabadash's modification. An immunohistochemical research method was used to detect a subpopulation of CD86+ cells in the liver of experimental animals. Under the influence of Vipera berus berus venom, the development of alterative changes in the liver parenchyma and vascular disorders were microscopically revealed. The appearance of foci of leukocyte infiltration was established, which indicates the development of inflammatory processes. A histochemical study of the content of succinate dehydrogenase in the liver of animals injected with Vipera berus berus venom showed uneven activity of the mitochondrial enzyme in the lobules. A histochemical study of glycogen in hepatocytes of the liver of rats exposed to Vipera berus berus venom showed a decrease in the content of trophic inclusions. An increase in the population of CD86+ cells indicates reactive inflammatory processes observed in the body as a result of the action of components of Vipera berus berus venom.

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Bothrops pauloensis snake venom-derived Asp-49 and Lys-49 phospholipases A2 mediates acute kidney injury by oxidative stress and release of inflammatory cytokines

Cited by 13 publications

References 50 publications

Photobiomodulation mitigates Bothrops jararacussu venom-induced damage in myoblast cells by enhancing myogenic factors and reducing cytokine production

Photobiomodulation mitigates Bothrops jararacussu venom-induced damage in myoblast cells by enhancing myogenic factors and reducing cytokine production

Crosstalk of Inflammation and Coagulation in Bothrops Snakebite Envenoming: Endogenous Signaling Pathways and Pathophysiology

Histological changes in the liver of rats under the influence of Vipera berus berus venom

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