Both the contribution of soil nitrogen and of biological N2 fixation to sugarcane can increase with the inoculation of diazotrophic bacteria

Martins, Doãn Sperandio; Reis, Verônica Massena; Schultz, Nivaldo; Alves, B. J. R.; Urquiaga, Segundo; Pereira, Willian; Sousa, Jailson Silva; Boddey, Robert M.

doi:10.1007/s11104-020-04621-1

Cited by 26 publications

(20 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Diazotrophs can be found in bulk soil, within the rhizosphere of plants, physically-associated with plant roots and other organs, and even inside plants within specialized, N fixing organs called nodules (Roy et al, 2020). Rates of BNF by free-living diazotrophs in soil are typically low, between 1 and 20 kg N ha −1 yr −1 (Vadakattu and Paterson, 2006), although associative N fixation by microbes in the rhizosphere or on plant surfaces may contribute significantly to plant growth in low-N input systems (e.g., Ladha and Reddy, 2003;Martins et al, 2020). In contrast, BNF in nodules is highly efficient, and in high-yield environments can exceed 300 kg N ha −1 yr −1 (Giller, 2001;Peoples et al, 2009;Cafaro La Menza et al, 2020) as nutrient exchange between plants and their intracellular bacterial endosymbionts is highly targeted (Udvardi and Poole, 2013), avoiding losses of plant-C and bacterialammonia to the soil and associated microbiome.…”

Section: Biological N Fixation (Bnf) and Incorporation Of Legumesmentioning

confidence: 99%

A Research Road Map for Responsible Use of Agricultural Nitrogen

Udvardi

Below

Castellano

et al. 2021

Front. Sustain. Food Syst.

View full text Add to dashboard Cite

Nitrogen (N) is an essential but generally limiting nutrient for biological systems. Development of the Haber-Bosch industrial process for ammonia synthesis helped to relieve N limitation of agricultural production, fueling the Green Revolution and reducing hunger. However, the massive use of industrial N fertilizer has doubled the N moving through the global N cycle with dramatic environmental consequences that threaten planetary health. Thus, there is an urgent need to reduce losses of reactive N from agriculture, while ensuring sufficient N inputs for food security. Here we review current knowledge related to N use efficiency (NUE) in agriculture and identify research opportunities in the areas of agronomy, plant breeding, biological N fixation (BNF), soil N cycling, and modeling to achieve responsible, sustainable use of N in agriculture. Amongst these opportunities, improved agricultural practices that synchronize crop N demand with soil N availability are low-hanging fruit. Crop breeding that targets root and shoot physiological processes will likely increase N uptake and utilization of soil N, while breeding for BNF effectiveness in legumes will enhance overall system NUE. Likewise, engineering of novel N-fixing symbioses in non-legumes could reduce the need for chemical fertilizers in agroecosystems but is a much longer-term goal. The use of simulation modeling to conceptualize the complex, interwoven processes that affect agroecosystem NUE, along with multi-objective optimization, will also accelerate NUE gains.

show abstract

Section: Biological N Fixation (Bnf) and Incorporation Of Legumesmentioning

confidence: 99%

A Research Road Map for Responsible Use of Agricultural Nitrogen

Udvardi

Below

Castellano

et al. 2021

Front. Sustain. Food Syst.

View full text Add to dashboard Cite

show abstract

“…This study was performed in pots that used total N balance and enriched 15 N fertilizer isotope-dilution methods, and the outcome was impressive. The findings of the 15 N enrichment indicated that there was a significant amount of non-labeled N present, which could only have originated from BNF ( Martins et al, 2020 ). Previous, investigations have found that Brazilian sugarcane cultivars were able to acquire 40–100 kg N ha –1 yr –1 from BNF utilizing plants without any treatment.…”

Section: Discussionmentioning

confidence: 99%

“…A significant development of plant growth and N accumulation using diazotrophic bacteria was also observed in different sugarcane varieties ( Li et al, 2017 ; Singh et al, 2020b ). However, even after three decades of research, our understanding as to how the diazotrophs work, fix N, and transfer the fixed N to the crop remains limited ( Martins et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…The utilization of biological N fixation (BNF) to minimize the input of N fertilizers in sugarcane has been reported, though the BNF microbes are not well characterized. Different bacterial genera such as Azotobacter , Azospirillum, Bacillus , Pseudomonas , and Enterobacter , have been shown to be associated with BNF ( Martins et al, 2020 ). The use of PGPEB to improve crop production may have advantages over epiphytic and rhizosphere bacteria as they are more vulnerable to soil and other external conditions ( James, 2000 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Root-Derived Endophytic Diazotrophic Bacteria Pantoea cypripedii AF1 and Kosakonia arachidis EF1 Promote Nitrogen Assimilation and Growth in Sugarcane

Singh

Guo

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

Excessive, long-term application of chemical fertilizers in sugarcane crops disrupts soil microbial flora and causes environmental pollution and yield decline. The role of endophytic bacteria in improving crop production is now well-documented. In this study, we have isolated and identified several endophytic bacterial strains from the root tissues of five sugarcane species. Among them, eleven Gram-negative isolates were selected and screened for plant growth-promoting characteristics, i.e., production of siderophores, indole-3-acetic acid (IAA), ammonia, hydrogen cyanide (HCN), and hydrolytic enzymes, phosphorus solubilization, antifungal activity against plant pathogens, nitrogen-fixation, 1-aminocyclopropane-1-carboxylic acid deaminase activity, and improving tolerance to different abiotic stresses. These isolates had nifH (11 isolates), acdS (8 isolates), and HCN (11 isolates) genes involved in N-fixation, stress tolerance, and pathogen biocontrol, respectively. Two isolates Pantoea cypripedii AF1and Kosakonia arachidis EF1 were the most potent strains and they colonized and grew in sugarcane plants. Both strains readily colonized the leading Chinese sugarcane variety GT42 and significantly increased the activity of nitrogen assimilation enzymes (glutamine synthetase, NADH glutamate dehydrogenase, and nitrate reductase), chitinase, and endo-glucanase and the content of phytohormones gibberellic acid, indole-3-acetic acid, and abscisic acid. The gene expression analysis of GT42 inoculated with isolates of P. cypripedii AF1 or K. arachidis EF1 showed increased activity of nifH and nitrogen assimilation genes. Also, the inoculated diazotrophs significantly increased plant nitrogen content, which was corroborated by the 15N isotope dilution analysis. Collectively, these findings suggest that P. cypripedii and K. arachidis are beneficial endophytes that could be used as a biofertilizer to improve plant nitrogen nutrition and growth of sugarcane. To the best of our knowledge, this is the first report of sugarcane growth enhancement and nitrogen fixation by Gram-negative sugarcane root-associated endophytic bacteria P. cypripedii and K. arachidis. These strains have the potential to be utilized as sugarcane biofertilizers, thus reducing nitrogen fertilizer use and improving disease management.

show abstract

“…Among gramineous crops (Poaceae), sugarcane appears to be the one that receives the most significant contribution from biological atmospheric nitrogen fixation (BNF), owing to the plant's ability to associate with diazotrophic bacteria (Martins et al 2020;Santos et al 2019;Taulé et al 2012;Urquiaga et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Strategy for the Sampling of Sugarcane Plants for the Reliable Quantification of N2 Fixation Using 15N Natural Abundance

Monteiro

Silva

Martins

et al. 2021

J Soil Sci Plant Nutr

Self Cite

View full text Add to dashboard Cite

The 15 N natural abundance (δ 15 N) technique offers good prospects for the quantification of the contribution of biological nitrogen fixation (BNF) to crop nutrition. The objectives of this study were to determine which plant parts best represented the whole plant with respect to δ 15 N and to quantify the BNF contribution to sugarcane. The experiment was carried out in pots of soil (100 kg pot −1 ) from the field. The sugarcane varieties used were RB867515 and RB92579 with the reference plants Brachiaria decumbens, millet (Pennisetum americanum) and sorghum (Sorghum bicolor). The experimental design was randomised blocks with four replications. The treatments were inoculation of the sugarcane with or without diazotrophic bacteria and the different parts of the sugarcane plant (root, green leaf, senescent leaf and different stem sections). The δ 15 N of different cane parts and varieties ranged from + 2.68 to + 5.85‰ after 365 days, while the δ 15 N of reference plants were from + 7.8 to + 8.3‰. The mean contribution of BNF to the sugarcane crop N supply was estimated to be 47% with no effect of variety or inoculation. The results indicate that the δ 15 N value of the entire shoot can be considered to represent the entire sugarcane plant, but no single organ could be relied on for this purpose.

show abstract

Both the contribution of soil nitrogen and of biological N2 fixation to sugarcane can increase with the inoculation of diazotrophic bacteria

Cited by 26 publications

References 39 publications

A Research Road Map for Responsible Use of Agricultural Nitrogen

A Research Road Map for Responsible Use of Agricultural Nitrogen

Root-Derived Endophytic Diazotrophic Bacteria Pantoea cypripedii AF1 and Kosakonia arachidis EF1 Promote Nitrogen Assimilation and Growth in Sugarcane

Strategy for the Sampling of Sugarcane Plants for the Reliable Quantification of N2 Fixation Using 15N Natural Abundance

Contact Info

Product

Resources

About