Both Max and TFE3 Cooperate with Smad Proteins to Bind the Plasminogen Activator Inhibitor-1 Promoter, but They Have Opposite Effects on Transcriptional Activity

Transcriptional Activation of Mouse Mast Cell Protease-7 by Activin and Transforming Growth Factor-β Is Inhibited by Microphthalmia-associated Transcription Factor

“…9A), was suggested to be the region responsible for Smad binding. The MH1 domain of Smad3 is responsible for physical interaction with TFE3, one of the members of the bHLHLZ family (48,49), and the leucine zipper domain of TFE3 interacts with the MH1 and linker region of Smad3 (49). These results suggest that the structural organization of the complex of Smad3 and MITF is distinct from that of Smad3 and TFE3.…”

“…MITF could form a complex with LEF-1, a signal mediator of the Wnt pathway, but the association was not sufficient for efficient transactivation of genes activated by the Wnt pathway (53). In addition, both Max, another member of the bHLHLZ family, and TFE3 bound to the MH1 region of Smad3 in vitro, but Max and TFE3 had opposite effects on Smad3-mediated transcriptional activation (49,54).…”

Synergistic Transcription Activation by Maf and Sox and Their Subnuclear Localization Are Disrupted by a Mutation in Maf That Causes Cataract

“…The chimeric protein MA1 contains Maf sequences on the N-terminal side of the leucine zipper (1 to 312) fused to the leucine zipper and C-terminal sequences of ATF2 (381 to 505). MA2 contains Maf sequences on the N-terminal side of the conserved basic region (1 to 298), including the unique GY dipeptide required for the extended DNA recognition specificity (15), fused to the conserved bZIP domain and C-terminal sequences of ATF2 (366 to 505). MA3 contains Maf sequences on the N-terminal side of the ancillary DNA binding region (1 to 265) fused to the bZIP domain and C-terminal sequences of of ATF2 (350 to 505).…”

Plasminogen activator inhibitor 1: Mechanisms of its synergistic regulation by growth factors

“…While TFE3 cooperates with Smad3/4 to activate transcription of TGF-β-responsive genes, Max inhibits Smad-mediated TGF-β-induced PAI-1 expression (Grinberg and Kerppola 2003). The same cis-elements can be activated in response to different stimuli.…”

“…For example, an E-box motif (CACGTG) located at -160 to -165 in the PAI-1 promoter mediates the transcriptional response to the helix-loop-helix family transcription factors that include TFE3 (transcription factor binding to IGHM enhancer 3), USF-1 (upstream transcription factor 1), USF-2 & HIF-1 (hypoxia-inducible factor 1) and Max (MYC associated factor X) (Hua, Liu et al 1998;Kietzmann, Roth et al 1999;Providence, White et al 2002;Grinberg and Kerppola 2003). While TFE3 cooperates with Smad3/4 to activate transcription of TGF-β-responsive genes, Max inhibits Smad-mediated TGF-β-induced PAI-1 expression (Grinberg and Kerppola 2003).…”