Both Maternal and Newborn IgMs Inhibit Transmissible Gastroenteritis Virus Internalization in LLC-PK1 Cells

Pogribna, A. P.; Haran, B.E.; Starosyla, D. B.; Рыбалко, С. Л.; Deryabin, O. M.; Syvak, V.V.; Govsieiev, D.О.

doi:10.15407/microbiolj85.02.060

Cited by 4 publications

(5 citation statements)

References 51 publications

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“…Статеві органи жінки мають власну систему, яка синтезує гуморальні фактори місцевого імунітету: секреторний імуноглобулін А (SІgA), IgA, IgG, IgM. SIgA виробляється в клітинах двох типів: у плазмоцитах -полімерний IgA з j-ланцюгами, а в епітеліальних клітинах -секреторний компонент, що дуже важливо в антибактеріальному і противірусному захисті [11,12,18,19].…”

Section: Original Articlesunclassified

“…Уже на початку вагітності відбуваються зміни в місцевому імунітеті. У дренуючих матку лімфатичних вузлах зростає число антитілоутворюючих клітин -збільшується кількість Т-супресорів, IgG і IgA, але кількість Т-хелперів при цьому змінюється незначно, спостерігається «фізіологічний дефіцит» плазматичних клітин, спрямований на попередження відторгнення ембріона та його подальший розвиток, що своєю чергою спонукає до розвитку часткового транзиторного імунодефіциту в системі «мати -плід» [9,11,[17][18][19].…”

Section: Original Articlesunclassified

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State of local immunity in women in labor with obstetric surgical wound infection in war conditions

Рrуlutska,

Avramenko,

Martynova

et al. 2024

UJPP

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Purpose - to study indicators of local immunity in the wound of women giving birth with an infection of an obstetric surgical wound in the conditions of war, which will give an opportunity to improve the therapy of wound infection at the present time. Materials and methods. Local immunity in the wound of 66 parturients with infected wounds during the war (the Group I) was studied and compared with indicators of local immunity in the wound of 76 parturients with infected wounds in peacetime (the Group II) six years to 2022. In addition, 15 healthy parturients - clinical remission (the Group III) were examined to determine the features of the course of the wound process. To study local immunity, the number of immunocompetent cells and phagocytic activity in biopsies from wounds, fixed immunoglobulins in smears-imprints were determined using the direct immunofluorescence method. Statistical data processing was carried out with the use of the application program package "Microsoft Excel 5.0" and the standard version "Statistica 8.0". The difference in indicators at Р<0.05 was considered statistically probable. Results and conclusions. In the examined women in labor with an infection of an obstetric surgical wound in the conditions of war under stressful conditions, there is a greater suppression of local immunity than in women in labor with this pathology in peacetime, which is expressed in the insufficient migration of immunocompetent cells to the focus of inflammation of the wound, especially B-lymphocytes, in the predominance of smears-imprints of plasma cells producing immunoglobulins of classes M and G, which lead to increased inflammation, inhibition of reparative processes in the wound, progression of the purulent-inflammatory process with the risk of septic complications during martial law, which leads to the correction of purulent wound therapy in obstetric practice during the war . The research was carried out in accordance with the principles of the Declaration of Helsinki. The research protocol was approved by the Local Ethics Committee of the institution mentioned in the work. Informed consent of the patients was obtained for the research.

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Section: Original Articlesunclassified

State of local immunity in women in labor with obstetric surgical wound infection in war conditions

Рrуlutska,

Avramenko,

Martynova

et al. 2024

UJPP

View full text Add to dashboard Cite

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“…За даними літератури, основним напрямом лікування гнійних ран є швидка ліквідація вогнища інфекції, яка досягається шляхом застосування методів сорбційної терапії, оскільки механізм їхньої дії в рані складається з капілярного дренажу, стимуляції процесів регенерації, сорбції мікробів та їхніх токсинів, ексудату, медіаторів запалення, ферментів, ендотоксинів. Також визначено, що складність проблеми лікування гнійних ран у породілей залежить від розвитку вторинної імунної недостатності, яка й обумовлює цілеспрямоване застосування методів імунопрофілактики та імунотерапії в комплексній терапії гнійних ран у породілей [2,3,6,8,12,13,[15][16][17][18][19]21,26,28].…”

Section: оригінальні дослідженняunclassified

Cytological and morphological predictors of complex treatment of obstetric surgical wound infection in women in labor

Рrуlutska,

Martynova,

Avramenko

et al. 2024

UJHW

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Purpose - to determination of the course and effectiveness of treatment of obstetric surgical wound infection in women in labor when using drugs with sorption-detoxification and immunomodulatory properties based on the study of the morphology of the wound process through the use of histological and cytological studies. Materials and methods. In the work, histological and cytological studies were used to monitor the course of the wound process in 115 parturients with purulent wounds of the anterior abdominal wall after caesarean section and perineum after episio- and perineotomy. Women are divided into groups depending on the method of treatment: the Group I - 42 parturients with purulent wounds, who were treated with organosilicon sorbent Gentaxan; the Group II - 45 women in labor with purulent wounds, who were treated with organosilicon sorbent Gentaxan and immunomodulator Laferon; the Group III - 28 women in labor with purulent wounds, who were treated with traditional methods - with subsequent statistical processing of the obtained results. Results. Based on the analysis of cytological and histological data, it was determined that the course of reparative processes in the wound during complex treatment with a sorbent and an immunomodulator is faster than that of monotherapy with a sorbent and conventional therapy - by 1.5-2 and 4-5 days, respectively. This was confirmed by a decrease in leukocytes, microorganisms, neutrophils and an increase in lymphocytes and connective tissue cells in the wound, affected the faster cleaning of the wound of dead and non-viable tissues, the rapid elimination of swelling and inflammation in the wound, and the acceleration of reparative processes. Conclusions. The complex use of an immunomodulator and a sorbent in the treatment of purulent wounds shows an advantage over sorbent monotherapy by accelerating the reparative processes in the wound by 1.5-2 days, and over traditional therapy by 4-5 days, which makes it possible to recommend them for use in practical medicine. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.

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“…Виникнення післяпологових гнійно-септичних ускладнень зумовлене як фактором інфікування, так і здатністю материнського організму протистояти інфекції. Під час вагітності вже виникає частковий транзиторний імунодефіцит, спрямований на підтримку імунної рівноваги в системі «мати-плід», а наявність несприятливих факторів, таких як застосування антибіотиків, гемотрансфузій, стероїдних гормонів, вакцин і сироваток, вплив несприятливих чинників навколишнього середовища, екстрагенітальна патологія, стрес, порушення харчування, операція, травма, ускладнення вагітності, призводять до імунодефіцитного стану, від якого залежить характер перебігу гнійно-запальних ускладнень [3,5,9,25,14,17,19].…”

Section: оригінальні дослідженняunclassified

The state of general immunity in women in labor with purulent wounds during hostilities

Рrуlutska,

Martynova,

Avramenko

et al. 2023

UJPP

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Purpose - to study the immune status of women giving birth with an infection of an obstetric surgical wound in the conditions of war, which will make it possible to develop and implement a pathogenetically justified method of wound infection therapy in the postpartum period. Materials and methods. We studied the immune status of 66 women in labor with an infection of an obstetric surgical wound in the conditions of war from February 24, 2022 and compared it with the indicators of general immunity of 76 women in labor with an infection of an obstetric surgical wound in peacetime. The material for the study of general immunity was peripheral blood, which was taken from the moment of infection of the obstetric surgical wound. To assess the state of general immunity, the total number of T lymphocytes (CD3+), their immunoregulatory subpopulations - T helpers/inducers (CD4+) and T suppressors/killers (CD8+), the number of B lymphocytes (CD22+), NK cells (CD16+) were counted), the immunoregulatory index (CD4+/CD8+) was calculated, the concentration of circulating immune complexes and immunoglobulins of classes A, M, G, phagocytic index and phagocytic number were determined. Statistical data processing was carried out using the package of application programs Microsoft Excel 5.0 and using the standard version Statistica 8.0. The difference in indicators was considered statistically significant at Р<0.05. Results. In women in labor with purulent wounds under the conditions of war under stressful conditions, there is a greater suppression of systemic immunity than in women in labor with this pathology in peacetime, which was manifested by T lymphopenia (decrease in CD3+ by 1.4 times, decrease in the level of T cells with helper activity (CD4+) by 1.2 times, immunoregulatory index (Tx/Tc) by 1.3 times), a decrease in the number of B lymphocytes (CD22+) and NK cells (CD16+) by an average of 1.2 times, a decrease indicators of immunoglobulins of classes A, M, G by an average of 1.3 times, phagocytic index) and phagocytic number relatively by 1.3 and 1.6 times, which led to an increase in the level of circulating immune complexes by 1.1 times. The absence of a significant difference between indicators of general immunity in parturient women with purulent wounds of the perineum and anterior abdominal wall was confirmed, which confirms the established doctrine that wounds of different localizations heal according to general laws and the body, namely the immune system, reacts equally to the wound process of any localization. Conclusions. When prescribing the treatment of an infection of an obstetric surgical wound, do not take into account the localization of the wound, since wounds of different localizations heal according to general laws and the immune system reacts in the same way. In women in labor with purulent wounds in wartime conditions, under stress, there is more suppression of systemic immunity than in women in labor with an infection of an obstetric surgical wound in peacetime, which increases the development and spread of the purulent-inflammatory process, increases the risk of severe septic complications, which leads to the development and implementation of a pathogenetically justified method of therapy of obstetric surgical wound infection in wartime conditions. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of the participating institution. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.

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Both Maternal and Newborn IgMs Inhibit Transmissible Gastroenteritis Virus Internalization in LLC-PK1 Cells

Cited by 4 publications

References 51 publications

State of local immunity in women in labor with obstetric surgical wound infection in war conditions

State of local immunity in women in labor with obstetric surgical wound infection in war conditions

Cytological and morphological predictors of complex treatment of obstetric surgical wound infection in women in labor

The state of general immunity in women in labor with purulent wounds during hostilities

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