Both Gut Microbiota and Differentially Expressed Proteins Are Relevant to the Development of Obesity

Abstract

Although the role of the gut microbiota in obesity has recently received considerable attention, the exact mechanism is unclear. This study was aimed at investigating the profiles of bacterial communities in fecal samples and differentially expressed proteins (DEPs) in the peripheral blood in mice fed a high-fat diet (HFD) and standard diet (SD) and at providing new insights into the pathogenesis of obesity. The profiles of bacterial communities in fecal samples and DEPs in the peripheral blood were characterized in mice fed HFD and SD, respectively. The levels of 3 DEPs increased in HFD mice. The alpha diversity was significantly lower after 4 and 12 weeks in HFD mice. The beta diversity was higher after 4, 8, and 12 weeks in HFD mice. A total of 16 gut bacterial clades were significantly different with the linear discriminant analysis (LDA) score higher than 4 over time. The relative abundance levels of Proteobacteria and Deferribacteres were higher, while those of Bacteroidetes and Firmicutes were lower in HFD mice at the phylum level. The relative abundance of Desulfovibrionaceae and Rikenellaceae increased in HFD mice at the family level. The relative abundance of the Bacteroidetes_S24-7_group and Lachnospiraceae was lower in HFD mice. The gut microbiota had a significant correlation with serum lipid indexes and expression of DEPs at the phylum and family levels. The changes in the gut microbiota of HFD mice and their associations with the levels of inflammatory proteins could be one of the major etiological mechanisms underlying obesity.

Dapagliflozin modulates the faecal microbiota after myocardial infarction in non‐diabetic mice

“…Our study proved that the treatment of dapagliflozin attenuated body weight in postinfarction mice significantly (compared with the MI group), which is also consistent. In addition, we found a negative correlation between Desulfovibrionaceae and body weight, which is opposite to the result that the increased Desulfovibrionaceae abundance may lead to glucose/lipid metabolic dysfunction, which is likely to promote body weight gain 41 …”

“…In addition, we found a negative correlation between Desulfovibrionaceae and body weight, which is opposite to the result that the increased Desulfovibrionaceae abundance may lead to glucose/lipid metabolic dysfunction, which is likely to promote body weight gain. 41 It was reported that SCFAs are more readily oxidized than ketones during heart failure. 42 However, whether dapagliflozin can attenuate cardiac dysfunction through faecal microbiota by generating SCFAs, or promoting ketogenesis, needs to be further clarified.…”

Dose-Dependent Effects of Histamine on Growth, Immunity and Intestinal Health in Juvenile Grouper (Epinephelus coioides)

“…As for horse study, supplementation of Saccharomyces cerevisiae (S. cerevisiae) modulated positively the overall structure of intestinal microbiota with increased relative abundance of Lachnospiraceae (Garber et al, 2020), which is a family of butyrate-producing bacteria (Nicholson et al, 2012;Hamilton et al, 2020). On the other hand, high fat diet resulted in a reduction of the relative abundance of the Bacteroidetes_S24-7_group and Lachnospiraceae in mice, which might be one of the primary etiological mechanisms underlying obesity (Li et al, 2020). Similar to this study, the lower relative abundance of Cetobacterium is accompanied by greater growth performance in tilapia (O. niloticus) (Standen et al, 2015;Li et al, 2019).…”

Bacillus amyloliquefaciens SC06 alleviates the obesity of ob/ob mice and improves their intestinal microbiota and bile acid metabolism

“…S24-7 is usually considered as an antiobesity gut microbiota, as it improves energy expenditure by producing butyrate. 56 However, some other studies have considered S24-7 to be a bad gut microbiota. 57,58 Matteo Serino et al found more S24-7 in mice fed with a high-fat carbohydrate-free diet, 58 which was in line with our present study.…”