Botanicals from the leaves of Acacia sieberiana had better cytotoxic effects than isolated phytochemicals towards MDR cancer cells lines

Ngaffo, Carine M.N.; Tchangna, Rodrigue S.V.; Mbaveng, Armelle T.; Kamga, Justin; Harvey, Freya M.; Ngadjui, Bonaventure T.; Bochet, Christian G.; Kuete, Victor

doi:10.1016/j.heliyon.2020.e05412

Cited by 23 publications

(9 citation statements)

References 46 publications

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“…To date, various anticancer flavonoids have been identified in TTE, such as apigenin, luteolin, chrysoeriol-7- O -β-D-glucopyranoside, and thalassiolin B [ 9 , 12 , 13 , 16 , 32 , 33 , 34 ]. Similar to TTE, apigenin treatment of SW480 cells shows comparable cell cytotoxicity (IC 50 value 40 µM) [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Marine Seagrass Extract of Thalassia testudinum Suppresses Colorectal Tumor Growth, Motility and Angiogenesis by Autophagic Stress and Immunogenic Cell Death Pathways

Hernández-Balmaseda¹,

Guerra²,

Declerck

et al. 2021

Marine Drugs

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Marine plants have become an inexhaustible reservoir of new phytopharmaceuticals for cancer treatment. We demonstrate in vitro/in vivo antitumor efficacy of a standardized polyphenol extract from the marine angiosperm Thalassia testudinum (TTE) in colon tumor cell lines (RKO, SW480, and CT26) and a syngeneic allograft murine colorectal cancer model. MTT assays revealed a dose-dependent decrease of cell viability of RKO, CT26, and SW480 cells upon TTE treatment with IC50 values of, respectively, 175, 115, and 60 μg/mL. Furthermore, TTE significantly prevented basal and bFGF-induced angiogenesis in the chicken chorioallantoic membrane angiogenesis assay. In addition, TTE suppressed bFGF-induced migration of endothelial cells in a wound closure assay. Finally, TTE treatment abrogated CT26 colorectal cancer growth and increased overall organism survival in a syngeneic murine allograft model. Corresponding transcriptome profiling and pathway analysis allowed for the identification of the mechanism of action for the antitumor effects of TTE. In line with our in vitro/in vivo results, TTE treatment triggers ATF4-P53-NFκB specific gene expression and autophagy stress pathways. This results in suppression of colon cancer cell growth, cell motility, and angiogenesis pathways in vitro and in addition promotes antitumor immunogenic cell death in vivo.

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Section: Discussionmentioning

confidence: 99%

Marine Seagrass Extract of Thalassia testudinum Suppresses Colorectal Tumor Growth, Motility and Angiogenesis by Autophagic Stress and Immunogenic Cell Death Pathways

Hernández-Balmaseda¹,

Guerra²,

Declerck

et al. 2021

Marine Drugs

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“…The leaves extract of Acacia sieberiana elicited anticancer activity (Ngaffo et al, 2020). The stem, roots, bark and leaves of Acacia sieberiana were reported to have antibacterial (Kirabo et al, 2018) and antidiarrhoeal actions [18,27].…”

Section: Introductionmentioning

confidence: 99%

“…The plant Acacia sieberiana var Woodii (Fabaceae) is a tree of 3-25 m in height and 0.6-1.8 m in diameter (Ngaffo et al, 2020). The bark is rough, yellowish and peels off in rectangular, small and grey-brown scales with gummy exudates.…”

Section: Introductionmentioning

confidence: 99%

“…In African traditional practices, the plant gained popularity in the treatment of many ailments. The roots decoction is utilized traditionally to treat bilharzia, bleeding, gonorrhoea, syphilis, rheumatism, kidney diseases, ophthalmia, stomach-aches, acne, tapeworms, urethral problems, and oedema (Ngaffo et al, 2020). The root decoction is also used in Nigeria to treat hepatitis (Ohemu et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…Previous phytochemical investigation on the Acacia sieberiana leaves leads to the isolation of gallic acid, kaempferol, ellagic acid, quercetin, isoferulic acid, kaempferol 3-α- L -arabinoside and quercetin 3-O-β- D -glucoside (Abdelhady, 2013). Other compounds including luteolin-7-O-rutinoside, chrysoeriol-7-O-rutinoside, apigenin-7-O-β- D -glucopyranoside, chrysoeriol-7-O-β- D -glucopyranoside, luteolin, luteolin-3′,4′-dimethoxylether-7-O-β- D -glucoside and sitosterol-3-O-β- D -glucoside, were isolated from the leaves of the plant (Ngaffo et al, 2020). The stem and leaves of Acacia sieberiana contain a glucoside dihydroacacipetalin and acacipetalin (Seigler et al, 1975).…”

Section: Introductionmentioning

confidence: 99%

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Toxicological study on methanol root bark extract ofAcacia sieberiana(Fabaceae) in Wistar rats

Watafua

Ejiofor

Musa

et al. 2022

Preprint

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Background: The plant Acacia sieberiana belongs to the family Fabaceae. It has been used in ethnomedical practice to manage bleeding, rheumatism, pain, pyrexia, kidney diseases, gastrointestinal problems, parasitic and infectious diseases, hepatitis, cough, epilepsy, mouth ulcer and many more. Phytochemical compounds such as ellagic acid, quercetin, isoferulic acid, gallic acid, kaempferol, luteolin, apigenin, glucoside dihydroacacipetalin, acacipetalin and many others were isolated from Acacia sieberiana. Previous pharmacological investigations have reported that the plant has anticancer, antimicrobial, antidiarrhoeal and antitrypanosomal effects. Despite the therapeutic properties of this plant, no safety information is available in the literature. Hence, this work intends to investigate the sub-acute toxicity effects of Acacia sieberiana root bark extract (ASE). The phytochemical and oral median lethal dose (LD50) evaluations on the ASE were done in line with the standard protocols. The sub-acute toxic effects of the ASE (250, 750, and 1,500 mg/kg) were investigated following administration of the ASE daily for 28-consecutive days based on the Organization of Economic Cooperation and Development (OECD) 407 protocols in rats. The weekly body weights were monitored and the rats were euthanized on the 29th day. The blood samples from the animals were obtained for biochemical and haematological determinations. The liver, kidney, lung and heart were removed for histological investigations.

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Variable intraspecific response to climate change in a medicinally important African tree species, Vachellia sieberiana (DC.) (paperbark thorn)

Jinga,

Manyangadze

2024

Ecology and Evolution

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Climate change is predicted to disproportionately impact sub‐Saharan Africa, with potential devastating consequences on plant populations. Climate change may, however, impact intraspecific taxa differently. The aim of the study was to determine the current distribution and impact of climate change on three varieties of Vachellia sieberiana, that is, var. sieberiana, var. villosa and var. woodii. Ensemble species distribution models (SDMs) were built in “biomod2” using 66, 45, and 137 occurrence records for var. sieberiana, var. villosa, and var. woodii, respectively. The ensemble SDMs were projected to 2041–2060 and 2081–2100 under three general circulation models (GCMs) and two shared socioeconomic pathways (SSPs). The three GCMs were the Canadian Earth System Model version 5, the Institut Pierre‐Simon Laplace Climate Model version 6A Low Resolution, and the Model for Interdisciplinary Research on Climate version 6. The suitable habitat of var. sieberiana predominantly occurs in the Sudanian and Zambezian phytochoria while that of var. villosa largely occurs in the Sudanian phytochorion. The suitable habitat of var. woodii mainly occurs in the Zambezian phyotochorion. There is coexistence of var. villosa and var. sieberiana in the Sudanian phytochorion while var. sieberiana and var. woodii coexist in the Zambezian phytochorion. Under SSP2‐4.5 in 2041–2060 and averaged across the three GCMs, the suitable habitat expanded by 33.8% and 119.7% for var. sieberiana and var. villosa, respectively. In contrast, the suitable habitat of var. woodii contracted by −8.4%. Similar trends were observed in 2041–2060 under SSP5‐8.5 [var. sieberiana (38.6%), var. villosa (139.0%), and var. woodii (−10.4%)], in 2081–2100 under SSP2‐4.5 [var. sieberiana (4.6%), var. villosa (153.4%), and var. woodii (−14.4%)], and in 2081–2100 under SSP5‐8.5 [var. sieberiana (49.3%), var. villosa (233.4%), and var. woodii (−30.7%)]. Different responses to climate change call for unique management and conservation decisions for the varieties.

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Botanicals from the leaves of Acacia sieberiana had better cytotoxic effects than isolated phytochemicals towards MDR cancer cells lines

Cited by 23 publications

References 46 publications

Marine Seagrass Extract of Thalassia testudinum Suppresses Colorectal Tumor Growth, Motility and Angiogenesis by Autophagic Stress and Immunogenic Cell Death Pathways

Marine Seagrass Extract of Thalassia testudinum Suppresses Colorectal Tumor Growth, Motility and Angiogenesis by Autophagic Stress and Immunogenic Cell Death Pathways

Toxicological study on methanol root bark extract ofAcacia sieberiana(Fabaceae) in Wistar rats

Variable intraspecific response to climate change in a medicinally important African tree species, Vachellia sieberiana (DC.) (paperbark thorn)

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