Botanical Investigation of Fallopia Dumetorum (L.) Holub (Polygonaceae) and Qualitative and Quantitative Assessment of its Polyphenolic Compounds

Abstract

Background: Considering the continuous need to fi nd new sources of polyphenolic compounds, we performed a pharmacognostical examination of the species Fallopia dumetorum (L.) Holub sin. Polygonum dumetorum L. (Polygonaceae). The plant is common in the plain regions of Romania and has not been exploited therapeutically. Materials and method: Microscopic examination was performed on cross-sections, surface preparations and on powder obtained from the aerial parts of the fl owering plant. Qualitative chemical analysis was realized by phytochemical screening and thin layer chromatography (TLC). Phenolic compounds were assayed by spectrophotometric methods: fl avonoids expressed as rutin (with aluminium chloride), phenolcarboxylic acids expressed as chlorogenic acid (Arnow's method) and proanthocyanidins expressed as cyanidin chloride (in acidic medium, by conversion to anthocyanins). Results:The species has the following microscopic characters: anomocytic stomata, druses of calcium oxalate, sessile, pluricellular glandular hairs and pollen grains with smooth exine. Polysaccharides, reducing compounds, coumarins, sterols/triterpenes, phenol-carboxylic acids, fl avones, proanthocyanidins, tannins and carotenoids were identifi ed by phytochemical screening; chlorogenic acid, caffeic acid, quercetin and stigmasterol/beta-sitosterol were detected by TLC. F. dumetori herba has a content of 1.49 ± 0.105 g% polyphenol-carboxylic acids, 0.40 ± 0.087g% fl avonoids and 0.18 ± 0.002 g% proanthocyanidins. Conclusions:We have characterized pharmacognostically the native species F. dumentorum. Due to its content in phenolic compounds it might serve as a source of polyphenols.

Anticancer potential of selected Fallopia Adans species

“…Three characteristic flavonoid structures have also been found in this species: falloconvolin A and B and quercetin-3-O-(2-E-esinapoxyl)-glucopyranoside (31). In F. aubertii, glycosides of quercetin, kaempferol, apigenin, luteolin, myricetin and several chromones structures have been identified (29,32,33). With the exception of rhamnetin, isorhamnetin and characteristic flavonoids, all other flavonoids have also been found in F. dumetorum (28).…”

“…In this study, we obtained six extracts from three plant species of the genus Fallopia. As F. convolvulus and F. dumetorum have a high TFC and TPC (42,43), we prepared only the hydroethanolic 50% extract (C ha and D ha ). From F. aubertii, four extracts were obtained: one from stems and leaves extracted with ethanol 50% (AH ha ) and three extracts from flowers with water (AF w ), ethanol 50% (AF ha ) and ethanol 96% (AF e ).…”

New N-acylhydrazones with Potential Cytotoxic Activity

“…The newly compounds were tested for potential cytotoxicity using an alternative methods on invertebrate organisms, Daphnia magna. This test is rapid, simple and cost-efficiently and can predict biological activities such as anticancer and analgesic activities [11,[18][19][20][21][22][23].…”

S-Alkylated 1,2,4-Triazoles Derivatives Synthesis, spectral analysis and cytotoxicity evaluation

“…The biological activity of the synthesized compounds was evaluated using Daphnia magna assay, an alternative method for prescreening of cytotoxicity activity [17,18]. Along with other bioassays, this method is widely used for toxicity risk assessment of new compounds, plant extracts, and pharmaceutical substances [19][20][21].…”