Bortezomib Facilitates Reparative Dentin Formation after Pulp Access Cavity Preparation in Mouse Molar

Jung, Jae‐Kwang; Gwon, Gi-Jeong; Neupane, Sanjiv; Sohn, Wern‐Joo; Kim, Ki-Rim; Kim, Ji-Youn; An, Shengli; Kwon, Tae‐Yub; An, Chang-Hyeon; Lee, Youngkyun; Ha, Jung‐Hong

doi:10.1016/j.joen.2017.07.018

Cited by 15 publications

(48 citation statements)

References 30 publications

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“…In particular, we performed RT‐qPCR analysis to evaluate the expression of bone formation‐related signaling molecules. The TGF‐β superfamily and Wnt genes are involved in bone formation and maintenance and have been recently reported to be upregulated with drug treatment that enhances reparative dentin formation . Therefore, we examined the expression patterns of TGF‐β superfamily and Wnt genes in the mesenchymal cells contributing to periodontium formation following OAA treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Mesenchymal tissue collection, hanging‐drop in vitro cultivation, and RT‐qPCR analysis were performed as previously described . Briefly, following dispase II treatment, tooth germ mesenchyme from 16 embryos was collected.…”

Section: Methodsmentioning

confidence: 99%

“…Ligated animals for the experimental procedures were anesthetized after 5 days, and the ligated maxillary left second molar was extracted to form a socket. The socket was treated with 2 µL of a mixture comprising Pluronic ® F‐127 (CAS: 9003‐11‐6, Sigma‐Aldrich, Germany) and 0.05% dimethyl sulfoxide (DMSO; CAS: 67‐68‐5, Duchefa Biochemie, the Netherlands) for the control groups and a 2‐µL mixture of OAA purified from Vigna angularis , Pluronic ® F‐127, and 0.05% DMSO for the drug repositioning experimental group . The final concentration of OAA in the mixture was maintained at 50 ng/µL with modifications in the previously reported method .…”

Section: Methodsmentioning

confidence: 99%

“…Mesenchymal tissue collection, hanging-drop in vitro cultivation, and RT-qPCR analysis were performed as previously described. 11…”

Section: In Vitro Mesenchymal Tissue Cultivation and Rt-qpcr Analysismentioning

confidence: 99%

“…However, primary and immortalized cell cultivations cannot represent the tissue interactions that play important roles in the developmental and regenerative processes. Therefore, in this study, an in vitro tissue culture method was employed to evaluate the precise signaling regulations in developing periodontium, based on our previous report . As described in the report, this in vitro tissue culture method reveals the underlying molecular mechanisms of test compound, oleanolic acid acetate (OAA), on the bone healing process.…”

Section: Introductionmentioning

confidence: 99%

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Effects of oleanolic acid acetate on bone formation in an experimental periodontitis model in mice

Adhikari

Neupane

Aryal

et al. 2019

J of Periodontal Research

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Objective We evaluated the role of oleanolic acid acetate (OAA), a triterpenoid commonly used in the treatment of liver disorders, inflammatory diseases, and metastasis, in bone formation after tooth loss by periodontitis. Background Periodontitis causes the sequential degradation of the alveolar bone and associated structures, resulting in tooth loss. Several studies have attempted to regenerate the bone for implantation following tooth loss. Methods Maxillary left second molar was extracted from 8‐week‐old male mice following induction of periodontitis by ligature for 5 days. The extraction socket was treated with 50 ng/µL OAA for 1, 2, and 3 weeks. Detailed morphological changes were examined using Masson's trichrome staining, and the precise localization patterns of various signaling molecules, including CD31, F4/80, interleukin (IL)‐6, and osteocalcin, were observed. The volume of bone formation was examined by Micro‐CT. Osteoclasts were enumerated using tartrate‐resistant acid phosphatase (TRAP) staining. For molecular dissection of signaling molecules, we employed the hanging‐drop in vitro cultivation method at E14 for 1 day and examined the expression pattern of transforming growth factor (TGF)‐β superfamily and Wnt signaling genes. Results Histomorphometrical examinations showed facilitated bone formation in the extraction socket following OAA treatment. In addition, OAA‐treated specimens showed the altered localization patterns of inflammatory and bone formation‐related signaling molecules including CD31, F4/80, IL‐6, and osteocalcin. Also, embryonic tooth germ mesenchymal tissue cultivation with OAA treatment showed the significant altered expression patterns of signaling molecules such as transforming growth factor (TGF)‐β superfamily and Wnt signaling. Conclusions Oleanolic acid acetate induces bone formation and remodeling through proper modulation of osteoblast, osteoclast, and inflammation with regulations of TGF‐β and Wnt signaling.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Mesenchymal tissue collection, hanging-drop in vitro cultivation, and RT-qPCR analysis were performed as previously described. 11…”

Section: In Vitro Mesenchymal Tissue Cultivation and Rt-qpcr Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of oleanolic acid acetate on bone formation in an experimental periodontitis model in mice

Adhikari

Neupane

Aryal

et al. 2019

J of Periodontal Research

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An endoplasmic reticulum stress regulator, Tmbim6, modulates secretory stage of mice molar

Aryal

Neupane

Adhikari

et al. 2019

Journal Cellular Physiology

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To understand the role of endoplasmic reticulum (ER)-stress in mice molar development, we studied Tmbim6 that antagonizes the unfolded protein response, using Tmbim6 knockout (KO) mice and in vitro organ cultivation with knocking down using small interfering RNA. During molar development, Tmbim6 is expressed in developing tooth at E14-E16, postnatal0 (PN0), and PN6. Mineral content in Tmbim6 KO enamel was reduced while dentin was slightly increased revealing ultrastructural changes in pattern formation of both enamel and dentin. Moreover, odontoblast differentiation was altered with increased Dspp expression at PN0 followed by altered AMELX localizations at PN5. These results were confirmed by in vitro organ cultivation and showed altered Bmp signaling, proliferation, and actin rearrangement in the presumptive ameloblast and odontoblasts that followed the altered expression of differentiation and ER stress-related signaling molecules at E16.5. Overall, ER stress modulated by Tmbim6 would play important roles in patterned dental hard tissue formation in mice molar within a limited period of development. K E Y W O R D Samelogenesis, dentinogenesis, ER stress, molar development, Tmbim6 knockout

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Developmental roles of glomerular epithelial protein-1 in mice molar morphogenesis

Neupane,

Aryal,

Kwak

et al. 2023

Cell Tissue Res

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Bortezomib Facilitates Reparative Dentin Formation after Pulp Access Cavity Preparation in Mouse Molar

Cited by 15 publications

References 30 publications

Effects of oleanolic acid acetate on bone formation in an experimental periodontitis model in mice

Effects of oleanolic acid acetate on bone formation in an experimental periodontitis model in mice

An endoplasmic reticulum stress regulator, Tmbim6, modulates secretory stage of mice molar

Developmental roles of glomerular epithelial protein-1 in mice molar morphogenesis

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