Borrelia burgdorferi sensu stricto and Borrelia afzelii : Population structure and differential pathogenicity

Jungnick, Sabrina; Margos, Gabriele; Rieger, Melissa; Dzaferovic, Eldina; Bent, Stephen J.; Overzier, Evelyn; Silaghi, Cornelia; Walder, Gernot; Wex, Franziska; Koloczek, J; Sing, Andreas; Fingerle, Volker

doi:10.1016/j.ijmm.2015.08.017

Cited by 43 publications

(32 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…have different infectivity properties, not only between but also within the genospecies, and this has direct implications on the epidemiology and risk assessment of human infections with these bacteria. While previous studies have showed the propensity of some STs of B. afzelii and B. burgdorferi to cause LB (Hanincova et al, 2013;Jungnick et al, 2015), our analysis indicates that at European scale the genetic diversity of Borrelia in humans is much higher than previously acknowledged, with 68 B. afzelii STs, of which 47 are newly described.…”

Section: Association With Human Cases Of Lyme Borreliosiscontrasting

confidence: 73%

Imbalanced presence of Borrelia burgdorferi s.l. multilocus sequence types in clinical manifestations of Lyme borreliosis

Coipan

Jahfari

Fonville

et al. 2016

Infection, Genetics and Evolution

View full text Add to dashboard Cite

In this study we used typing based on the eight multilocus sequence typing scheme housekeeping genes (MLST) and 5S-23S rDNA intergenic spacer (IGS) to explore the population structure of Borrelia burgdorferi sensu lato isolates from patients with Lyme borreliosis (LB) and to test the association between the B. burgdorferi s.l. sequence types (ST) and the clinical manifestations they cause in humans. Isolates of B. burgdorferi from 183 LB cases across Europe, with distinct clinical manifestations, and 257 Ixodes ricinus lysates from The Netherlands, were analyzed for this study alone. For completeness, we incorporated in our analysis also 335 European B. burgdorferi s.l. MLST profiles retrieved from literature. Borrelia afzelii and Borrelia bavariensis were associated with human cases of LB while Borrelia garinii, Borrelia lusitaniae and Borrelia valaisiana were associated with questing I. ricinus ticks. B. afzelii was associated with acrodermatitis chronica atrophicans, while B. garinii and B. bavariensis were associated with neuroborreliosis. The samples in our study belonged to 251 different STs, of which 94 are newly described, adding to the overall picture of the genetic diversity of Borrelia genospecies. The fraction of STs that were isolated from human samples was significantly higher for the genospecies that are known to be maintained in enzootic cycles by mammals (B. afzelii, B. bavariensis, and Borrelia spielmanii) than for genospecies that are maintained by birds (B. garinii and B. valaisiana) or lizards (B. lusitaniae). We found six multilocus sequence types that were significantly associated to clinical manifestations in humans and five IGS haplotypes that were associated with the human LB cases. While IGS could perform just as well as the housekeeping genes in the MLST scheme for predicting the infectivity of B. burgdorferi s.l., the advantage of MLST is that it can also capture the differential invasiveness of the various STs.

show abstract

Section: Association With Human Cases Of Lyme Borreliosiscontrasting

confidence: 73%

Imbalanced presence of Borrelia burgdorferi s.l. multilocus sequence types in clinical manifestations of Lyme borreliosis

Coipan

Jahfari

Fonville

et al. 2016

Infection, Genetics and Evolution

View full text Add to dashboard Cite

show abstract

“…species complex [77], and on the population structure and pathogenicity of B. afzelii and B. burgdorferi ( s.s .) [78]. To our knowledge, this is the first study using MLST for the detection of allelic combinations of B. burgdorferi in small mammals from Germany.…”

Section: Discussionmentioning

confidence: 99%

The enzootic life-cycle of Borrelia burgdorferi (sensu lato) and tick-borne rickettsiae: an epidemiological study on wild-living small mammals and their ticks from Saxony, Germany

et al. 2017

View full text Add to dashboard Cite

Background Borrelia burgdorferi (sensu lato) and rickettsiae of the spotted fever group are zoonotic tick-borne pathogens. While small mammals are confirmed reservoirs for certain Borrelia spp., little is known about the reservoirs for tick-borne rickettsiae. Between 2012 and 2014, ticks were collected from the vegetation and small mammals which were trapped in Saxony, Germany. DNA extracted from ticks and the small mammals’ skin was analyzed for the presence of Rickettsia spp. and B. burgdorferi (s.l.) by qPCR targeting the gltA and p41 genes, respectively. Partial sequencing of the rickettsial ompB gene and an MLST of B. burgdorferi (s.l.) were conducted for species determination.ResultsIn total, 673 small mammals belonging to eight species (Apodemus agrarius, n = 7; A. flavicollis, n = 214; Microtus arvalis, n = 8; Microtus agrestis, n = 1; Mustela nivalis, n = 2; Myodes glareolus, n = 435; Sorex araneus, n = 5; and Talpa europaea, n = 1) were collected and examined. In total, 916 questing ticks belonging to three species (Ixodes ricinus, n = 741; Dermacentor reticulatus, n = 174; and I. trianguliceps, n = 1) were collected. Of these, 474 ticks were further investigated. The prevalence for Rickettsia spp. and B. burgdorferi (s.l.) in the investigated small mammals was 25.3 and 31.2%, respectively. The chance of encountering Rickettsia spp. in M. glareolus was seven times higher for specimens infested with D. reticulatus than for those which were free of D. reticulatus (OR: 7.0; 95% CI: 3.3–14.7; P < 0.001). In total, 11.4% of questing I. ricinus and 70.5% of D. reticulatus were positive for Rickettsia spp. DNA of B. burgdorferi (s.l.) was detected only in I. ricinus (5.5%). Sequence analysis revealed 9 R. helvetica, 5 R. raoultii, and 1 R. felis obtained from 15 small mammal samples.ConclusionSmall mammals may serve as reservoirs for Rickettsia spp. and B. burgdorferi (s.l.). While the prevalence for Rickettsia spp. in M. glareolus is most likely depending on the abundance of attached D. reticulatus, the prevalence for B. burgdorferi (s.l.) in small mammals is independent of tick abundance. Dermacentor reticulatus may be the main vector of certain Rickettsia spp. but not for Borrelia spp.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-017-2053-4) contains supplementary material, which is available to authorized users.

show abstract

“…The use of sequence information renders it unambiguous and portable (Urwin and Maiden, 2003). Even though whole genome sequencing is readily available and more and more cost-effective, traditional MLST performed by Sanger sequencing is still routinely used, easy available and provides sufficient information to resolve many questions, particularly for bacteria that are difficult to cultivate (Jungnick et al, 2015;Mukhacheva and Kovalev, 2013).…”

mentioning

confidence: 99%

PubMLST.org – The new home for the Borrelia MLSA database

Margos

Binder²,

Dzaferovic³

et al. 2015

Ticks and Tick-borne Diseases

Self Cite

View full text Add to dashboard Cite

Borrelia burgdorferi sensu stricto and Borrelia afzelii : Population structure and differential pathogenicity

Cited by 43 publications

References 37 publications

Imbalanced presence of Borrelia burgdorferi s.l. multilocus sequence types in clinical manifestations of Lyme borreliosis

Imbalanced presence of Borrelia burgdorferi s.l. multilocus sequence types in clinical manifestations of Lyme borreliosis

The enzootic life-cycle of Borrelia burgdorferi (sensu lato) and tick-borne rickettsiae: an epidemiological study on wild-living small mammals and their ticks from Saxony, Germany

PubMLST.org – The new home for the Borrelia MLSA database

Contact Info

Product

Resources

About