Borrelia burgdorferi bind to epithelial cell proteoglycans.

Isaacs, Robin

doi:10.1172/jci117035

Cited by 100 publications

(109 citation statements)

References 49 publications

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“…In addition, the B314 transformants did not bind to cultured endothelial cells, an activity that we previously showed was associated with the recognition of heparan sulfate (49). Heparin is more negatively charged than heparan sulfate, and the ability to bind commercial preparations of heparin does not uniformly correspond to an ability to bind heparan sulfate (15). Nevertheless, it is possible that heparan sulfate GAGs contribute to DbpB-mediated attachment to some cell types, because complete inhibition of C6 glioma cell attachment by the DbpB-expressing recombinant strain required removal of heparan sulfate in addition to chondroitin and dermatan sulfate GAGs (J.R.F., unpublished observation).…”

Section: Discussionmentioning

confidence: 99%

“…Consistent with the ability of B. burgdorferi to cause multisystemic infection, the spirochete can attach to a variety of cell types (8)(9)(10)(11)(12)(13). Proteoglycans, a class of ubiquitously expressed host cell molecules, are among the mammalian cell components that are recognized by this pathogen (14)(15)(16). Proteoglycans consist of a core protein covalently linked to one or more glycosaminoglycan (GAG) chains (for review, see ref.…”

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confidence: 96%

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Decorin-binding proteins A and B confer distinct mammalian cell type-specific attachment by Borrelia burgdorferi , the Lyme disease spirochete

Fischer

Parveen

Magoun

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

109

149

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Host cell binding is an essential step in colonization by many bacterial pathogens, and the Lyme disease agent, Borrelia burgdorferi, which colonizes multiple tissues, is capable of attachment to diverse cell types. Glycosaminoglycans (GAGs) are ubiquitously expressed on mammalian cells and are recognized by multiple B. burgdorferi surface proteins. We previously showed that B. burgdorferi strains differ in the particular spectrum of GAGs that they recognize, leading to differences in the cultured mammalian cell types that they efficiently bind. The molecular basis of these binding specificities remains undefined, due to the difficulty of analyzing multiple, potentially redundant cell attachment pathways and to the paucity of genetic tools for this pathogen. In the current study, we show that the expression of decorin-binding protein (

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 96%

Decorin-binding proteins A and B confer distinct mammalian cell type-specific attachment by Borrelia burgdorferi , the Lyme disease spirochete

Fischer

Parveen

Magoun

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

109

149

View full text Add to dashboard Cite

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“…), in spite of extensive expansion of the spirochete population, indicates that host factors other than those associated with specific immunity interfere with gene expression in spirochetes. Although elusive so far, it is possible that host molecules such as fibronectin [26], proteoglycan [29,35], glycosphingolipids [4], decorin [17], complement regulators [31], plasminogen [12,21] and integrins [11], which serve as receptors for spirochetal outer surface proteins, participate in this process(es).…”

Section: Discussionmentioning

confidence: 99%

Quantitative analysis of Borrelia burgdorferi gene expression in naturally (tick) infected mouse strains

Lederer

Brenner

Stehlé

et al. 2004

Med Microbiol Immunol

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Adaptation of Borrelia burgdorferi in the vector and vertebrate host is mediated by mechanisms that regulate differential expression of outer surface lipoproteins (Osps). In this study, real time PCR was applied to quantify tissue-specific expression of four linear plasmid (lp54)-encoded (ospA, zs7.a36, zs7.a66 zs7.a68) and one circular plasmid (cp26)-encoded (ospC) gene from B. burgdorferi sensu stricto, in a natural setting of tick-infected immunodeficient (C.B-17 SCID) and immunocompetent (BALB/c and AKR/OlaHsd) mice for up to 120 days post-infection (p.i.). Early during infection (day 30 p.i.) high numbers of spirochetes were found in the heart and joint, but not the ear and spleen tissues of disease-susceptible SCID mice. In diseasesusceptible AKR mice spirochetes colonized the ear and joint tissues, but were undetectable in tissues of diseaseresistant BALB/c mice. Later in infection (day 120 p.i.), spirochetes had expanded ($1,000-fold) in all SCID tissues tested but were undetectable in AKR and BALB/ c mice. Of the five genes analyzed, only zs7.a36 transcripts were detected in various tissues of all infected mouse strains, though at differing levels, whereas ospC transcripts were only found in tissue specimens of SCID mice. Furthermore, gene expression of ospC and zs7.a36 appears to be differentially regulated in distinct organs of individual mice. In contrast, transcripts for ospA, zs7.a66, and zs7.a68 were not detected in any of the mouse strains, independent of their immune status and/ or the severity of their infection/inflammatory responses. Late during infection (day 120 p.i.), transcription of zs7.a36 and ospC was down-regulated in the tissues of SCID mice despite expansion of spirochetes. This type of quantitative analysis may be helpful to further disclose principles of pathogenesis of Lyme borreliosis and to design strategies for its therapeutic treatment.

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“…All mammalian cells were obtained from the American Type Culture Collection (ATCC) and cultured as previously described [7][8][9][10][11] . Briefly, cells were cultured in suspension in 75 mm 2 tissue culture flasks with core media of RPMI 1640 (Jurkat E6-1, PC12), MEM (HEK-293, HeLa) cells, or F12K(CHO-K1) cells.…”

Section: Representative Resultsmentioning

confidence: 99%

Determination of Mammalian Cell Counts, Cell Size and Cell Health Using the Moxi Z Mini Automated Cell Counter

Dittami

Sethi

Rabbitt

et al. 2012

JoVE

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Particle and cell counting is used for a variety of applications including routine cell culture, hematological analysis, and industrial controls [1][2][3][4][5] . A critical breakthrough in cell/particle counting technologies was the development of the Coulter technique by Wallace Coulter over 50 years ago. The technique involves the application of an electric field across a micron-sized aperture and hydrodynamically focusing single particles through the aperture. The resulting occlusion of the aperture by the particles yields a measurable change in electric impedance that can be directly and precisely correlated to cell size/volume. The recognition of the approach as the benchmark in cell/particle counting stems from the extraordinary precision and accuracy of its particle sizing and counts, particularly as compared to manual and imaging based technologies (accuracies on the order of 98% for Coulter counters versus 75-80% for manual and vision-based systems). This can be attributed to the fact that, unlike imaging-based approaches to cell counting, the Coulter Technique makes a true three-dimensional (3-D) measurement of cells/particles which dramatically reduces count interference from debris and clustering by calculating precise volumetric information about the cells/particles. Overall this provides a means for enumerating and sizing cells in a more accurate, less tedious, less time-consuming, and less subjective means than other counting techniques 6 .Despite the prominence of the Coulter technique in cell counting, its widespread use in routine biological studies has been prohibitive due to the cost and size of traditional instruments. Although a less expensive Coulter-based instrument has been produced, it has limitations as compared to its more expensive counterparts in the correction for "coincidence events" in which two or more cells pass through the aperture and are measured simultaneously. Another limitation with existing Coulter technologies is the lack of metrics on the overall health of cell samples. Consequently, additional techniques must often be used in conjunction with Coulter counting to assess cell viability. This extends experimental setup time and cost since the traditional methods of viability assessment require cell staining and/or use of expensive and cumbersome equipment such as a flow cytometer.The Moxi Z mini automated cell counter, described here, is an ultra-small benchtop instrument that combines the accuracy of the Coulter Principle with a thin-film sensor technology to enable precise sizing and counting of particles ranging from 3-25 microns, depending on the cell counting cassette used. The M type cassette can be used to count particles from with average diameters of 4 -25 microns (dynamic range 2 -34 microns), and the Type S cassette can be used to count particles with and average diameter of 3 -20 microns (dynamic range 2 -26 microns). Since the system uses a volumetric measurement method, the 4-25 microns corresponds to a cell volume range of 34 -8,180 fL and the 3 -...

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Borrelia burgdorferi bind to epithelial cell proteoglycans.

Abstract: Invest. 1994. 93:809-819.)

Cited by 100 publications

References 49 publications

Decorin-binding proteins A and B confer distinct mammalian cell type-specific attachment by Borrelia burgdorferi , the Lyme disease spirochete

Decorin-binding proteins A and B confer distinct mammalian cell type-specific attachment by Borrelia burgdorferi , the Lyme disease spirochete

Quantitative analysis of Borrelia burgdorferi gene expression in naturally (tick) infected mouse strains

Determination of Mammalian Cell Counts, Cell Size and Cell Health Using the Moxi Z Mini Automated Cell Counter

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