Boronic Acid-Functionalized Core–Shell–Shell Magnetic Composite Microspheres for the Selective Enrichment of Glycoprotein

Abstract

In this work, core-shell-shell-structured boronic acid-functionalized magnetic composite microspheres Fe3O4@SiO2@poly (methyl methacrylate-co-4-vinylphenylbornoic acid) (Fe3O4@SiO2@P(MMA-co-VPBA)) with a uniform size and fine morphology were synthesized. Here, Fe3O4 magnetic particles were prepared by a solvothermal reaction, whereas the Fe3O4@SiO2 microspheres with a core-shell structure were obtained by a sol-gel process. 3-(Trimethoxysilyl) propyl methacrylate (MPS)-modified Fe3O4@SiO2 was used as the seed in the emulsion polymerization of MMA and VPBA to form the core-shell-shell-structured magnetic composite microspheres. As the boronic acid groups on the surface of Fe3O4@SiO2@P(MMA-co-VPBA) could form tight yet reversible covalent bonds with the cis-1,2-diols groups of glycoproteins, the magnetic composite microspheres were applied to enrich a standard glycoprotein, horseradish peroxidase (HRP), and the results demonstrated that the composite microspheres have a higher affinity for the glycoproteins in the presence of the nonglycoprotein bovine serum albumin (BSA) over HRP. Additionally, different monomer mole ratios of MMA/VPBA were studied, and the results implied that using MMA as the major monomer could reduce the amount of VPBA with a similar glycoprotein enrichment efficiency but a lower cost.

Preparation of poly(vinylphenylboronic acid) chain grafted poly(glycidylmethacrylate-co-ethylenedimethacrylate) beads for the selective enrichment of glycoprotein

“…S3 and revealed that the amount of OVA bound to the beads at equilibrium, Q , increased as the initial concentration of OVA increased. The static absorption data showed that the beads possessed a higher binding capacity for the analyte, and the maximum adsorption content was 31.4 mg/g in the OVA concentration range of 0.08–1.0 mg/mL, which was higher than other boronated materials .…”

Polymer-Functionalized Magnetic Nanoparticles: Synthesis, Characterization, and Methylene Blue Adsorption

“…Fe 3 O 4 @SiO 2 MNPs were synthesized by Stöber method according to the previous researches [13,16]. Fe 3 O 4 (1.2 g) was homogeneously dispersed in the mixture of anhydrous ethanol (400 mL), ultrapure water (200 mL), and ammonia solution (6 mL).…”

Tumor‐Targeting Multifunctional Rattle‐Type Theranostic Nanoparticles for MRI/NIRF Bimodal Imaging and Delivery of Hydrophobic Drugs

“…First, the Fe 3 O 4 nanoparticles were prepared though a hydrothermal method ( Figure 1 a). [ 41 ] These nanoparticles had a hydrodynamic diameter of 221 nm and polydisperse index (PDI) of 0.21 measured by DLS (Figure 1 d). The uniformity of these nanoparticles as the cores was responsible of the size distribution of the fi nal naorattles.…”