Boron-Doped Nanocrystalline Diamond Electrodes for Neural Interfaces: In vivo Biocompatibility Evaluation

Alcaide, María José; Taylor, Andrew; Fjorback, Morten Voss; Zachar, Vladimír; Pennisi, Cristian Pablo

doi:10.3389/fnins.2016.00087

Cited by 40 publications

(29 citation statements)

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“…NCD3 layer has high content of boron and has therefore increased conductivity (13,7 Ωm). As shown in previous study 5 , the boron content and increased conductivity should have no impact on the neuron development.…”

Section: Effect Of the Surface Roughness Of Diamond Layerssupporting

confidence: 66%

“…The successful construction of neuroelectrodes is mainly given by the ability to create an efficient interface between the electrode material and neural cells or tissues. Recently, diamond became an atractive material of choice for construction of neuroelectrodes 1,2 and neurointerfaces [3][4][5][6][7] and their applications as retinal prosthesis 8,9 . The material characteristics of diamond combines several aspects that could improve the performance of neuroelectrodes.…”

Section: Introductionmentioning

confidence: 99%

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Neuron Adhesion on Diamond: Competition between Polymer Treatment and Surface Morphology

et al. 2018

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The ability to form an efficient interface between material and neural cells is a crucial aspect of the construction of neuroelectrodes. Diamond offers material characteristics that could, by a large extent, improve the performance of neuroelectrodes. The greatest advantage of diamond is a large variety of material and surface properties such as electrical conductivity, surface morphology, and surface chemistry. Such a variety of material characteristics can lead to various cellular responses. Here we compare survival, adhesion, and neurite formation of primary neurons on diamond thin films of various morphology and on their treatment with several types of polymers commonly used to enhance cell adhesion. We found that the variation of surface roughness of nanocrystalline diamond film does not have a major influence on the neuron survival or adhesion. The adhesion of neurons can beinfluenced by the selected type of polymer coating.

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Section: Effect Of the Surface Roughness Of Diamond Layerssupporting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Neuron Adhesion on Diamond: Competition between Polymer Treatment and Surface Morphology

et al. 2018

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“…Concluding, a fibrous capsule can be reduced, when a suitable electrode architecture is provided. Related to 2D electrodes with several materials 22 , the reduced capsule formation of the porous electrode implies a reduced foreign body reaction. In conclusion, the 3D nano fiber electrode integrates well into a host tissues without the induction of an adverse foreign body response.…”

Section: Discussionmentioning

confidence: 99%

A three-dimensional hybrid pacemaker electrode seamlessly integrates into engineered, functional human cardiac tissue in vitro

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Schmitz

Pfister

et al. 2018

Sci Rep

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Pacemaker systems are an essential tool for the treatment of cardiovascular diseases. However, the immune system’s natural response to a foreign body results in the encapsulation of a pacemaker electrode and an impaired energy efficiency by increasing the excitation threshold. The integration of the electrode into the tissue is affected by implant properties such as size, mechanical flexibility, shape, and dimensionality. Three-dimensional, tissue-like electrode scaffolds render an alternative to currently used planar metal electrodes. Based on a modified electrospinning process and a high temperature treatment, a conductive, porous fiber scaffold was fabricated. The electrical and immunological properties of this 3D electrode were compared to 2D TiN electrodes. An increased surface of the fiber electrode compared to the planar 2D electrode, showed an enhanced electrical performance. Moreover, the migration of cells into the 3D construct was observed and a lower inflammatory response was induced. After early and late in vivo host response evaluation subcutaneously, the 3D fiber scaffold showed no adverse foreign body response. By embedding the 3D fiber scaffold in human cardiomyocytes, a tissue-electrode hybrid was generated that facilitates a high regenerative capacity and a low risk of fibrosis. This hybrid was implanted onto a spontaneously beating, tissue-engineered human cardiac patch to investigate if a seamless electronic-tissue interface is generated. The fusion of this hybrid electrode with a cardiac patch resulted in a mechanical stable and electrical excitable unit. Thereby, the feasibility of a seamless tissue-electrode interface was proven.

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“…Such biomaterial seems to be well suited for neural interface since it combines the diamond electrochemical properties, providing good neural stimulation and recording performance, with the structural stability of the nano-structure. The concerns about the cytotoxicity were addressed by some in vitro and in vivo (subcutaneous implant up to 4 weeks) experiments that have shown good biocompatibility together with lower inflammation response and smaller fibrous capsule formation (Piret et al, 2015 ; Alcaide et al, 2016 ).…”

Section: Possible Strategies To Reduce Fbrmentioning

confidence: 99%

Invasive Intraneural Interfaces: Foreign Body Reaction Issues

et al. 2017

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Intraneural interfaces are stimulation/registration devices designed to couple the peripheral nervous system (PNS) with the environment. Over the last years, their use has increased in a wide range of applications, such as the control of a new generation of neural-interfaced prostheses. At present, the success of this technology is limited by an electrical impedance increase, due to an inflammatory response called foreign body reaction (FBR), which leads to the formation of a fibrotic tissue around the interface, eventually causing an inefficient transduction of the electrical signal. Based on recent developments in biomaterials and inflammatory/fibrotic pathologies, we explore and select the biological solutions that might be adopted in the neural interfaces FBR context: modifications of the interface surface, such as organic and synthetic coatings; the use of specific drugs or molecular biology tools to target the microenvironment around the interface; the development of bio-engineered-scaffold to reduce immune response and promote interface-tissue integration. By linking what we believe are the major crucial steps of the FBR process with related solutions, we point out the main issues that future research has to focus on: biocompatibility without losing signal conduction properties, good reproducible in vitro/in vivo models, drugs exhaustion and undesired side effects. The underlined pros and cons of proposed solutions show clearly the importance of a better understanding of all the molecular and cellular pathways involved and the need of a multi-target action based on a bio-engineered combination approach.

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Boron-Doped Nanocrystalline Diamond Electrodes for Neural Interfaces: In vivo Biocompatibility Evaluation

Cited by 40 publications

References 50 publications

Neuron Adhesion on Diamond: Competition between Polymer Treatment and Surface Morphology

Neuron Adhesion on Diamond: Competition between Polymer Treatment and Surface Morphology

A three-dimensional hybrid pacemaker electrode seamlessly integrates into engineered, functional human cardiac tissue in vitro

Invasive Intraneural Interfaces: Foreign Body Reaction Issues

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