Bordetella hinzii sp. nov., Isolated from Poultry and Humans

Abstract

A polyphasic taxonomic study that included DNA-rRNA hybridizations, DNA-DNA hybridizations, DNA base ratio determinations, whole-cell protein and fatty acid analyses, and an examination of classical phenotypic characteristics was performed in order to classify human and veterinary isolates that resemble Burdeteh avium. Twelve poultry isolates and two human isolates were assigned to a new species, for which we propose the name BordeteZliz hinzii. The position of this organism in the family Akaligenuceae and various genotypic, phenotypic, and chemotaxonomic characteristics are described.Members of the genus Bordetella are well-known pathogens of humans and animals (39). The significance of Bordetella pertussis, Bordetella parapertussis, and Bordetella bronchiseptica as respiratory tract invaders, with whooping cough as the most important infection, is well-known (39). A less familiar member of the genus, Bordetella avium, causes respiratory infections in poultry (22). These infections result in high levels of morbidity and are responsible for significant losses in both the turkey and chicken industries (3, 6, 17, 28, 32, 33). Strains of another taxon, which has been referred to as B. avium-like, Alcaligenes faecalis type 11, TC (turkey coryza) bacterium type 11, or Alcaligenes sp. strain C2T2, have also been isolated from respiratory tracts of chickens and turkeys in various parts of the world (1,3,19,20,31). Although these strains are often isolated from diseased birds, the information available does not provide strong evidence that they are pathogenic (3,19).In this study, we performed a polyphasic taxonomic study in order to clarify the taxonomic position of the B. avium-like taxon. Below, we show that 12 poultry isolates and 2 human isolates of the B. avium-like taxon constitute a novel Bordetella species, for which we propose the name Bordetella hinzii sp. nov. MATERIALS AND METHODSBacterial strains and growth conditions. All of the strains which we studied and their sources are listed in Table 1. Bacteriological purity was checked by plating and examining living cells, using phase-contrast microscopy and Gramstained cells.B. pertussis strains were grown as described by Stainer and Scholte (34). All other strains were grown on Trypticase soy agar (catalog no. 11768; BBL, Becton Dickinson Microbiology Systems, Cockeysville, Md.) and were incubated aerobically at 36 to 37°C unless indicated otherwise.PAGE of whole-cell proteins. After incubation for 48 h, whole-cell protein extracts were prepared, and sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) was performed as described previously (30). A densitometric analysis, normalization and interpolation of the protein profiles, and a numerical analysis were performed by using the GelCompar software package (Applied Maths, Kortrijk, Belgium).Fatty acid methyl ester analysis. After incubation for 48 h, a loopful of well-grown cells was harvested, and fatty acid methyl esters were prepared, separated, and identified by using the Microbial Identif...

Bordetella petrii from a clinical sample in Australia: isolation and molecular identification

“…Bordetella bronchiseptica causes respiratory tract infections in many mammals and only rarely infects humans (Woolfrey & Moody, 1991;Gueirard et al, 1994). Bordetella hinzii has been isolated from immunocompromised human hosts, but is mainly a commensal of the respiratory tract of poultry (Cookson et al, 1994;Vandamme et al, 1995;Funke et al, 1996). Bordetella avium, a bird pathogen, is the only Bordetella species that has never been found in humans (Arp & Cheville, 1984).…”

Temporal bacterial diversity and detection of putative methanotrophs in surface mats of Lonar crater lake

“…The LAII-2 clone sequence was distantly related to Bordetella hinzii (AF177667) with 93.1% homology and is the only Betaproteobacteria associated clone found in the entire study. The association of Bordetella hinzii with avian respiratory infections has been reported earlier [34] but its possible role in the mat ecosystem is difficult to deduce as the phylogenetic identity is low.…”

Streptococcus difficile Is a Nonhemolytic Group B, Type Ib Streptococcus

“…The results of numerical analyses of one-dimensional wholeorganism protein fingerprints are often equivalent to the results obtained by DNA-DNA hybridization, as strains with very similar whole-cell protein patterns for the most part exhibit high DNA-DNA binding values. This equivalence has been widely documented in gram-negative bacteria, including the genera Alcaligenes, Arcobacter, Bordetella, Campylobacter, and Pseudomonas (19,27,28,30). In gram-positive bacteria, this relationship is less well documented because of the difficulty of obtaining sufficient amounts of DNA for the hybridization experiments, yet it has been demonstrated in several genera, including the genera Clostridium, Enterococcus, Lactobacillus, Lactococcus, and Paenibacillus (4, 6, 13-15, 17, 21, 26 iniae , Streptococcus pnrusu nguis , Streptococcus para u beris , and S. phocue) have been included in DNA-DNA hybridization and protein electrophoretic studies ( Fig.…”