Borated Titania, a New Option for the Selective Enrichment of cis‐Diol Biomolecules

Abstract

As low abundance cis-diol biomolecules are of great significance in biological organisms, preparation of materials for the selective enrichment of such compounds is highly favorable for the development of the related proteomics and metabolomics. To this end, we have prepared monolithic borated titania by a non-aqueous sol-gel strategy as a new inorganic affinity material for the specific capture of nucleosides, glycopeptides and glycoproteins. Benefiting from the inorganic framework, this material prevented the hydrophobic interference, which was somewhat inevitable for the mainstream organic-based boronate affinity materials. The prepared material was carefully characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and nitrogen-sorption experiments to investigate the morphology and elemental composition. The excellent performance of borated titania on enrichment of cis-diol biomolecules was demonstrated by extracting the glycopeptides from horseradish peroxidase (HRP) digestion, standard glycoproteins, and nucleosides from a human-urine matrix. This kind of inorganic affinity material offers a new option for selective enrichment or separation of cis-diol biomolecules.

A high boronate avidity monolithic capillary for the selective enrichment of trace glycoproteins

“…Existing tools for the selective enrichment of glycoproteins mainly include lectins [4][5][6][7], antibodies [8,9], hydrazide [10][11][12][13][14], and boronate affinity materials [15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34]. However, lectins and antibodies are associated with apparent disadvantages, such as difficult to prepare and poor stability.…”

“…As compared with the above competing approaches, boronate affinity materials can provide several significant advantages such as low cost, class selectivity, easy-to-manipulate capture/release (through pH switch) and good compatibility with mass spectrometry. Boronic acid-functionalized materials, such as macroporous monoliths [17,18,[21][22][23]25,28,29,[31][32][33][34], and nanoparticles [16,20,24], have been developed into important tools for the facile selective extraction of glycoproteins. However, conventional boronate affinity materials suffer from an apparent limitation.…”

Development of Monolithic Column Materials for the Separation and Analysis of Glycans

“…Fused silica capillaries with inner diameter that ranges from 75 µm to 530 µm were the most commonly used packing material in these monolithic columns [82-85,87-94,96,98,99,102-105, 107-110,114,115,117-120,123,144]. Other types such as stainless steel tube [95,111,121], poly(etherether-ketone) (PEEK) column [146], pipette tips [100,101] and syringe [106] have also been reported.…”

“…At pH 8.0, high selectivity was achieved, represented by the big difference in binding capacity between glycoprotein Ova and non-glycoprotein BSA as compared to same binding capacity when at acidic media [105]. In another work, a completely inorganic borated titania monolith prepared via sol-gel process, and packed in a syringe was used in selective extraction of glycoproteins from fresh egg white [106].…”

A selective pretreatment method for determination of endogenous active brassinosteroids in plant tissues: double layered solid phase extraction combined with boronate affinity polymer monolith microextraction

“…This capture/release principle has drawn much attention in sample pretreatment filed. Boronate affinity magnetic nanoparticles, bonded silica and polymer monoliths have been prepared for the capture and separation of catechol, carbonhydrates, RNA, nucleosides, glycoproteins and glycopeptides [15-21]. As BRs possess two pairs of cis-diols at C 2 , C 3 and C 22 , C 23 position (Figure  2), boronate affinity chromatography could be a powerful technique for the selective isolation of BRs from plant matrix.…”