Boraksın (Na2O2B2O3.10H2O) Tenebrio mollitor (Coleoptera: Tenebrionidae) Üzerindeki LC50 Değerinin Belirlenmesi ve Boraksın Subletal Dozlarının Un Kurdu Larvaları Üzerindeki Toksik Etkilerinin Belirlenmesi

Abstract

In this study, lethal concentration 50 (LC50) value of borax on Tenebrio mollitor (Coleoptera: Tenebrionidae) was determined with testing 100, 1.000, 10.000, 100.000 ppm borax doses. The LC50 value was determined after 20 days later as 3.402,15 ppm for larvae and 28.512,5 ppm for adults. Also sublethal 100, 1.000, 3.000 ppm borax doses were tested to determine the effects on the larval weight of insect and the adverse effects on the hemocytes. When at other doses except 100 ppm, a decrease was observed in the larval weight means compared to the control group, also a statistically significant decrease was observed in the 3.000 ppm test group compared to the control group. Finally, apoptosis, necrosis and micronucleus formations were determined by Giemsa staining technique in hemocytes of groups exposed to high doses (1.000 ppm and 3.000 ppm borax).