Boosting Influences From circ_0048856 On Non-Small Cell Lung Cancer Advancement Via Aiming At miR-1287-5p

Abstract

Background: Non small cell lung cancer (NSCLC) is one of the main causes of death in human diseases. In this study, we aimed to explore the prognostic value of serum circ_0048856 in patients with NSCLC and its relationship with miR-1287-5p.Methods: The expression levels of circ_0048856 and miR-1287-5p in serum of NSCLC patients and healthy people were detected by qRT-PCR. The relationships between circ_0048856 expression and clinical data were evaluated via χ2 test. Overall survival analysis was carried out via Kaplan-Meier method with log rank test. Cox regression analysis was taken to evaluate the prognostic ability of circ_0048856 in NSCLC. Luciferase reporter gene was used to detect the targeting relationship between circ_0048856 and miR-1287-5p.Results: The expression of circ_0048856 in serum of NSCLC patients was significantly higher than that of healthy volunteers (P < 0.001). And its high expression was significantly correlated with TNM stage (P = 0.002) and lymph node metastasis (P < 0.001). In addition, Kaplan Meier log rank test analysis showed that the overall survival of patients with high expression of circ_0048856 was significantly shorter than that of patients with low expression of circ_0048856 (log rank test, P = 0.002). Serum circ_0048856 expression is an independent prognostic biomarker in NSCLC patients. Furthermore, we found that miR-1287-5p is a direct downstream miRNA of circ_0048856 through luciferase reporter gene analysis, and there is a negative correlation between circ_0048856 and miR-1287-5p.Conclusion: Serum circ_0048856 is an independent prognostic biomarker in patients with NSCLC. Circ_0048856 can participate in the progression of NSCLC by targeting miR-1287-5p.