The aim of this study is to determine the depression, anxiety, and stress levels of those who apply for unemployment insurance benefits using the DASS-21 scale, which is an important social policy aiming to partially and temporarily compensate the income loss of the employees whose job was terminated, and to shed light on the policies to be created in this direction. For this purpose, the research was carried out with a total of 479 individuals, 279 of whom were women and 280 were men, who applied for Unemployment İnsurance Benefits at Turkiye Employment Agency (İŞKUR) Eskişehir Provincial Directorate between September 1-30, 2022. The data obtained from this study shows that there is no significant difference in the levels of depression, anxiety, and stress according to the gender of the participants. Considering the same case in terms of marital status and education level, it was observed that depression, anxiety, and stress levels were higher if the individuals were married, and the education level of the individuals was higher. In addition, it has been observed that the depression, anxiety, and stress levels of individuals who work in the same workplace for longer periods than the shorter ones displayed higher levels of emotions on the measurements while applying for unemployment insurance benefits.