“…It is reported to be slow‐acting, difficult to apply uniformly in some species, and may not be appropriate for invasive procedures (Ross & Ross, ; Trushenski et al., ; Mylniczenko, Neiffer, & Clauss, ). The blood gas and acid‐base balance of a fish are altered when using CO 2 , thus sodium bicarbonate should be added for its buffering effect (Mylniczenko et al., ); but again, this adds an unapproved substance to the sedative solution (Trushenski et al., ). CO 2 is currently the only immediate‐release sedative available in the USA; although not approved by the FDA, use of CO 2 is considered a drug of “low regulatory priority”, and fish may be released immediately after sedation (Trushenski, Bowzer, Bowker, & Schwarz, ).…”