Bone tissue scaffolds based on antimicrobial SiO2–Na2O–Al2O3–CaO–B2O3 glass

Influence of dissolution products of a novel Ca-enriched silicate bioactive glass-ceramic on VEGF release from bone marrow stromal cells

“…Considering this result and the role of intracellular calcium in neovascularization from previous studies, there is scope for further long-term ion release studies and to perform further angiogenesis assays with these granules, which might give a better understanding of the time dependant interaction between calcium release and angiogenic growth factors. Bioactivity, biocompatibility and antibacterial properties of this glass-ceramic, in the form of granules and scaffolds, have been previously demonstrated [20,21]. In combination with the present results, it can be stated that the developed glass-ceramic granules have the potential to be applied as bone substitute materials.…”

“…The chemical composition in wt% is: 43. [20,21]. The starting glass powder was axially pressed into cylindrical pellets and heat treated at 850 ∘ C during 1h in air atmosphere and subsequently milled and separated in three different granular sizes: SmS (0.5 -1.0 mm), MeS (1.0-2.0 mm) and LaS (2.0-3.15 mm).…”

“…The starting glass powder was axially pressed into cylindrical pellets and heat treated at 850 ∘ C during 1h in air atmosphere and subsequently milled and separated in three different granular sizes: SmS (0.5 -1.0 mm), MeS (1.0-2.0 mm) and LaS (2.0-3.15 mm). The thermal treatment at 850 ∘ C was selected based on a previous study [21] in which scaffolds were sintered at different temperatures using this glass powder. The obtained scaffolds were analyzed to determine their bioactivity, antimicrobial properties and mechanical resistance.…”

“…The obtained scaffolds were analyzed to determine their bioactivity, antimicrobial properties and mechanical resistance. The scaffold sintered at 850 ∘ C showed the highest antimicrobial properties and the highest compressive strength [20,21].…”

“…This glass was originally developed as an antibacterial material [20] and its bioactivity in simulated body fluid has also been confirmed [21]. In this study, ST-2 bone marrow stromal cells were cultivated with the supernatant of different granules of different sizes and at different concentrations (0-1 wt/vol % of granules) for 24 h. In addition to in vitro studies, Ca-ion release from all granules as a main dissolution product of the investigated glass was analysed.…”