Bone Resorption Inhibitor Alendronate Normalizes the Reduced Bone Thickness of TRPV5−/− Mice

Nijenhuis, Tom; Eerden, Bram C. J. van der; Hoenderop, Joost G. J.; Weinans, Harrie; Leeuwen, Johannes P.T.M. van; Bindels, René J. M.

doi:10.1359/jbmr.080613

Cited by 27 publications

(26 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Anti-resorption drugs, such as bisphosphonates, selective estrogen receptor modulators, and hormone therapy have been used to treat osteoporosis for years. These agents suppress bone resorption and bone turnover, resulting in a relative increase of bone formation and bone mineral density and subsequently lowering fracture rates in hip and spine [7]. However, there seems to be limited information available with regard to the long-term usage of oral bisphosphonates.…”

Section: Introductionmentioning

confidence: 99%

High-frequency loading positively impacts titanium implant osseointegration in impaired bone

et al. 2014

View full text Add to dashboard Cite

Summary High-frequency loading via whole body vibration promotes bone formation and increases bone strength. Whether this translates to positive titanium implant osseointegration in osteoporotic bone was explored in this animal study. An anabolic effect of not only bisphosphonate treatment but also high-frequency loading on implant osseointegration in osteoporotic bone was observed. Introduction The present study investigated the impact of high-frequency (HF) loading, applied via whole body vibration (WBV), on titanium implant osseointegration in healthy versus ovariectomyinduced compromised versus pharmacologically treated compromised bone. Methods A custom-made Ti implant was inserted into the metaphyseal tibia of 59 rats and left to heal for either 4 or 14 days. Rats were divided into six groups according to their hormonal and mechanical status. WBV, consisting of 10 consecutive frequency steps at an acceleration of 0.3g, was applied daily for either 4 or 14 days. Tissue samples were processed for quantitative histology at the tibial cortical and medullar level. Data were analyzed by three-way ANOVA and by post hoc pairwise comparisons. Results The bone healing response at the interface and surrounding titanium implants was negatively influenced by osteoporotic bone conditions, mainly at the trabecular bone level. Furthermore, the administration of bisphosphonates for preventing the ovariectomy-induced impaired periimplant response was successful. Finally, the effect of HF WBV loading on the peri-implant bone healing was dependent on the bone condition and was anabolic solely in untreated osteoporotic trabecular bone when applied for an extended period of time. ConclusionsThe bone healing response to implant installation is compromised in osteoporotic bone conditions, in particular at the trabecular bone compartment. Meanwhile, not only pharmacological treatment but also mechanical loading via HF WBV can exert a positive effect on implant osseointegration in this specific bone micro-environment. The peri-implant cortical bone, however, seems to be less sensitive to HF WBV loading influences.

show abstract

Section: Introductionmentioning

confidence: 99%

High-frequency loading positively impacts titanium implant osseointegration in impaired bone

et al. 2014

View full text Add to dashboard Cite

show abstract

“…103 Bindels et al demonstrated the role of Klotho in the regulation of TRPV5. [104][105][106][107][108][109][110] They also found that Klotho is expressed in high amount in kidney and co-localizes with TRPV5 (Fig. 3).…”

Section: Meeting Reportmentioning

confidence: 79%

Transient Receptor Potential Channels: What is happening? Reflections in the wake of the 2009 TRP meeting, Karolinska Institutet, Stockholm

Goswami¹,

Islam²

2010

Channels

View full text Add to dashboard Cite

Role of TRP channels in thermosensation.The abilities of sensing and differentiating environmental temperatures are important functions performed by almost all organisms. However, different organisms handle this fundamental function by different mechanisms and by recruiting different sets of molecules that can sense different temperatures. 6,7 In this context, TRP channels are particularly interesting since some TRP channels can be activated by different temperatures.8-10 Such thermo-sensitive TRP channels are quite selective for different temperature ranges in which they can be activated.11 Such temperature ranges cover from noxious cold to noxious heat. It has, therefore, been speculated that the thermo-sensitive TRP channels are responsible for thermosensation and for the maintenance of the normal body temperature.12-14 However, the molecular mechanisms by which these thermo-sensitive TRP channels recognize different temperatures remain unanswered.15,16 Recent electrophysiological and knockout studies show that the molecular mechanism of thermosensation can not be solely dependent on the TRP channels. Molecules other than TRP channels are also involved in this process in a more complex manner than it was thought before. [17][18][19][20][21] In fact many of the animals lacking TRP channels show normal thermosensation. 22,23 On the other hand, some animals genetically lacking molecules other than the TRP channels also manifest altered thermosensation. For example mice lacking P2Y2 receptor reveals abnormal thermal nociception. 24 Taking together, these studies indicate that a complex network is operating behind the molecular mechanisms of thermosensation.Previously it has been shown that swapping the C-terminal cytoplasmic domain of a cold receptor (TRPM8) with a hot receptor (TRPV1) makes the cold receptor recognize high temperature and vice versa. 25 This indicated that the C-terminal TRP channelsWhat's happening? Reflections in the wake of the 2009 TRP Meeting, Karolinska Institutet, Stockholm

show abstract

“…showing that the number of resorption pits was reduced in TRPV5 (-/-) bone marrow cultures (Nijenhuis et al, 2008).…”

Section: Discussionmentioning

confidence: 96%

Inhibition of bone resorption by econazole in rat osteoclast-like cells through suppressing TRPV5

et al. 2011

View full text Add to dashboard Cite

Osteoclasts are primary bone resorption cells and intervention in osteoclast activation is considered an effective therapeutic approach to treatment of bone diseases involving osteoclasts. TRPV5 was detected in osteoclasts and it has been thought to take part in the transportation of the degraded calcium in the resorption lacuna, which is essential for bone resorption. The aim of the present study was to examine the effects of a modulator of calcium dynamics, econazole, on the expression of TRPV5 and bone resorption activity in rat osteoclast-like cells (OLCs). OLCs were obtained by co-culturing rat bone marrow cells with osteoblasts and then culturing with different concentrations of econazole (0.01, 0.1, 1.0, 10.0 μmol/L). Cell counting and staining protocols were used to determine whether econazole influenced the survival of OLCs. Expression of TRPV5 in response to econazole treatment was assessed by western blotting. Bone resorption activity of OLCs was determined by measuring the resorption area of dentin slices with a microscope and a digital image analysis system. Additionally, Ca(2+) inside OLCs was tested. We found that econazole inhibited expression of TRPV5 in a dose dependent manner while it had no influence on the survival of OLCs and it therefore inhibited bone resorption activity in rat OLCs. Ca(2+) inside OLCs increased, suggesting a limited compensatory mechanism to make up for inhibition of TRPV5 effects.

show abstract

Bone Resorption Inhibitor Alendronate Normalizes the Reduced Bone Thickness of TRPV5−/− Mice

Cited by 27 publications

References 53 publications

High-frequency loading positively impacts titanium implant osseointegration in impaired bone

High-frequency loading positively impacts titanium implant osseointegration in impaired bone

Transient Receptor Potential Channels: What is happening? Reflections in the wake of the 2009 TRP meeting, Karolinska Institutet, Stockholm

Inhibition of bone resorption by econazole in rat osteoclast-like cells through suppressing TRPV5

Contact Info

Product

Resources

About