Bone Resorption in Tissue Culture. Factors Influencing the Response to Parathyroid Hormone *

Raisz, Lawrence G.

doi:10.1172/jci105117

Cited by 376 publications

(114 citation statements)

References 28 publications

Supporting

Mentioning

111

Contrasting

Unclassified

Order By: Relevance

“…Bone resorbing activity, as a result of OAF in the leukocyte culture supernates, was assessed by methods which have been previously ...... 45 described (13,17). Pregnant mice were rejected subcutaneously with 0.05 mCl of Ca on the 16th d of gestation.…”

Section: Bioassay For Bone Resorbing Activity As a Results Of Oafmentioning

confidence: 99%

Monocytes regulate osteoclast-activating factor production by releasing prostaglandins.

Yoneda¹,

Mundy²

1979

The Journal of Experimental Medicine

129

View full text Add to dashboard Cite

Osteoclast-activating factor (OAF), a powerful stimulator of osteoclastic bone resorption, is released by peripheral blood mononuclear cells on exposure to phytohemagglutinin (PHA) or a specific antigen to which the leukocytes have been previously exposed. Both lymphocytes and monocytes are required in the leukocyte population for OAF release to occur. In this study we examined the relationship between the lymphocyte and monocyte in OAF production. Biological activity, as a result of OAF, was assessed by a bioassay based on the release of previously incorporated 45Ca from fetal rodent long bones in organ culture. We found that an enriched lymphocyte population depleted of monocytes by serial adherence does not release OAF after stimulation with PHA, although the cells are activated as assessed by [3H]thymidine and 3H-amino acid incorporation. When conditioned media harvested from adherent cells which did not contain OAF was added to the enriched lymphocytes, OAF release occurred. Media harvested from adherent cells which were cultured with indomethacin (10 microM), an inhibitor of prostaglandin synthesis, did not permit OAF release by activated lymphocytes. When PGE1 and PGE2 (0.1 microM) were added exogenously to the enriched lymphocyte population, OAF release occurred after stimulation with PHA. These results indicate that, (a) the activated lymphocyte is the cell or origin of OAF, (b) prostaglandins produced by monocytes are necessary for OAF production by activated lymphocytes, and (c) monocyte prostaglandins can influence bone resorption indirectly by regulating OAF production as well as directly by osteoclast activation. The interactions of OAF and prostaglandins at bone resorbing sites may be important in inflammatory and neoplastic diseases associated with bone destruction.

show abstract

Section: Bioassay For Bone Resorbing Activity As a Results Of Oafmentioning

confidence: 99%

Monocytes regulate osteoclast-activating factor production by releasing prostaglandins.

Yoneda¹,

Mundy²

1979

The Journal of Experimental Medicine

129

View full text Add to dashboard Cite

show abstract

“…Therefore, it was logical to think that significant OAF production in vitro could be the best indication of early myeloma osteoclastic activity. Unfortunately, OAF production is evaluated in vitro by a very complex bioassay procedure based on short-term liquid cultures of myeloma cells (Raisz, 1965). This difficulty was well illustrated by conflicting results recently published by authors who tried to relate bone resorbing activity measured in vitro to the presenting features of myeloma patients (especially the extent of bone lesions and disease activity) (Schecter et al, 1980;Durie et al, 1981).…”

mentioning

confidence: 99%

“…Supernatants derived from the short-term suspension cultures were boiassayed for bone resorbing activity using the radiolabelled bone resorption assay described by Raisz (1965). Briefly, pregnant rats were injected i.p.…”

mentioning

confidence: 99%

In vitro osteolytic activity of human myeloma plasma cells and the clinical evaluation of myeloma osteoclastic bone lesions

Jf¹,

Bataille²

1984

Br J Cancer

View full text Add to dashboard Cite

“…In an attempt to express the bone resorption more quantitatively, a modification was made to this system by measuring the release of previously incorporated Ca4s from the calvariae into the medium according to the method of Raisz (1965). The combination of the two parameters, gross appearance of the calvariae stained by the Von Kossa method and the release of previously incorporated Ca45 in the medium enabled us to show the small difference in the bone resorption much more quantitatively.…”

Section: Discussionmentioning

confidence: 99%

Pyrophosphate Enhancement of Thyrocalcitonin Effect in Tissue Culture

1969

View full text Add to dashboard Cite

SynopsisIn vitro bone culture technique was developed by the modification of the Goldhaber's method. Using this method, pyrophosphate was found to inhibit PTH induced bone resorption as judged by the release of previously incorporated Ca45 into the medium and by the histological findings. Furthermore, addition of pyrophosphate markedly enhanced the thyrocalcitonin inhibition of bone resorption in this system. These findings, together with the previous findings that pyrophosphate enhances the hypocalcemic response to TC in vivo, strongly suggest the favorable effect of the combined use of pyrophosphate and TC in the bone diseases characterized by excessive bone resorption.

show abstract

Bone Resorption in Tissue Culture. Factors Influencing the Response to Parathyroid Hormone *

Cited by 376 publications

References 28 publications

Monocytes regulate osteoclast-activating factor production by releasing prostaglandins.

Monocytes regulate osteoclast-activating factor production by releasing prostaglandins.

In vitro osteolytic activity of human myeloma plasma cells and the clinical evaluation of myeloma osteoclastic bone lesions

Pyrophosphate Enhancement of Thyrocalcitonin Effect in Tissue Culture

Contact Info

Product

Resources

About