Bone Repair Analysis in a Novel Biodegradable Hydroxyapatite/Collagen Composite Implanted in Bone

Nishikawa, Tetsunari; Masuno, Kazuya; Tominaga, Kazuhiro; Koyama, Yoshihisa; Yamada, Terutane; Takakuda, Kazuo; Kikuchi, Masanori; Tanaka, Junzo; Tanaka, Akio

doi:10.1097/01.id.0000173628.00705.d0

Cited by 25 publications

(18 citation statements)

References 22 publications

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“…In the past, newly developed bone substitutes have been tested in vivo using numerous experimental models, most of them involving larger animals such as dogs,16 goats,17 or rabbits 18. In our study, we have used the dorsal skinfold chamber model of Syrian golden hamsters, which bears the advantages of convenient handling and lower costs.…”

Section: Discussionmentioning

confidence: 99%

Injectable nanocrystalline hydroxyapatite paste for bone substitution: In vivo analysis of biocompatibility and vascularization

et al. 2007

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The nanocrystalline hydroxyapatite paste Ostim represents a fully degradable synthetic bone substitute for the filling of bone defects. Herein, we investigated in vivo the inflammatory and angiogenic host tissue response to this biomaterial after implantation. For this purpose, Ostim was implanted into the dorsal skinfold chambers of Syrian golden hamsters. The hydroxyapatite ceramic Cerabone and isogeneic transplanted cancellous bone served as controls. Angiogenesis, microhemodynamics, microvascular permeability, and leukocyte-endothelial cell interaction of the host tissue were analyzed over 2 weeks using intravital fluorescence microscopy. Ostim exhibited good biocompatibility comparable to that of Cerabone and cancellous bone, as indicated by a lack of venular leukocyte activation after implantation. Cancellous bone induced a more pronounced angiogenic response and an increased microvessel density when compared with the synthetic bone substitutes. In contrast to Cerabone, however, Ostim showed a guided neovascularization directed toward areas of degradation. Histology confirmed the ingrowth of proliferating vascularized tissue into the hydroxyapatite paste at sites of degradation, while the hydroxyapatite ceramic was not pierced by new microvessels. Thus, Ostim represents an injectable synthetic bone substitute, which may optimize the conditions for the formation of new bone at sites of bone defects by supporting a guided vascularization during biodegradation.

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Section: Discussionmentioning

confidence: 99%

Injectable nanocrystalline hydroxyapatite paste for bone substitution: In vivo analysis of biocompatibility and vascularization

et al. 2007

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“…In the study by Fukui et al (2008), the nanoHAp/collagen composites were prepared by soaking honeycomb collagen sponge in a nano-HAp suspension to obtain a microstructure of pore sizes in the range of 200-350 µm in the honeycomb collagen sponge with a HAp content in the range of 2 to 10 wt%. The animal studies (Nishikawa et al, 2005;Fukui et al, 2008;Zhou et al, 2006) indicated that the HAp/collagen composite implants induced new bone growth and was replaced by the newly formed bone tissue. Thus, nano-HAp/collagen composite is bioresorptive and is attractive as a scaffold material.…”

Section: Introductionmentioning

confidence: 96%

“…First, we utilize a first-principles computational method to compute the mechanical properties of nano-scaled HAp particles. Nano-scaled particles have been chosen because it is biocompatible, biodegradable, and has been utilized as implants in bone repair in a dog model (Nishikawa et al, 2005) and in a rabbit model (Fukui et al, 2008;Zhou et al, 2006). In the study by Fukui et al (2008), the nanoHAp/collagen composites were prepared by soaking honeycomb collagen sponge in a nano-HAp suspension to obtain a microstructure of pore sizes in the range of 200-350 µm in the honeycomb collagen sponge with a HAp content in the range of 2 to 10 wt%.…”

Section: Introductionmentioning

confidence: 99%

A multiscale modeling approach to scaffold design and property prediction

Chan

Liang

Francis

et al. 2010

Journal of the Mechanical Behavior of Biomedical Materials

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“…Microbeads of HA/Col were fabricated into disc shapes, which promoted healing of holes in rats' calvaria. As well, HA/Col composite is a useful implant material for bone augmentation in the femora and tibiae of dogs18; however, a HA disc did not integrate with peripheral bone tissue by the 24th week after implantation 19. Microbeads are suitable substrates for culturing cells in vitro to generate biohybrid scaffolds for tissue repair and gene therapy 19…”

Section: Introductionmentioning

confidence: 97%

Characterization and osteogenic effects of mesenchymal stem cells on microbeads composed of hydroxyapatite nanoparticles/reconstituted collagen

Jeng

Chung

Lin

et al. 2008

J Biomedical Materials Res

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Natural bone is comprised of nanosized blade-like crystals of hydroxyapatite grown in close contact with collagen (Col) fibers. Characteristics of artificial bone tissue differ considerably with those of natural ones, mainly from the unusual self-organizing interaction between the apatite crystals and the proteic components. Nanoparticle spheres of hydroxyapatite (n-HA), dispersed in reconstituted fibrous Col, were prepared in three weight ratios of 75:25, 65:35, and 50:50 (n-HA:Col). Bone marrow mesenchymal stem cells (MSCs) from rabbits were seeded and cultured on the n-HA/Col microbeads and characterized. n-HA were evenly distributed throughout the Col matrix and aggregated to microbeads as determined by scanning electron microscopy. Electron and confocal microscopy showed that the MSCs spread and attached to microbeads via focal adhesions, while staining for F-actin and DNA revealed the presence of stress fibers. The phenotype of the MSCs in the flow cytometry was identified as CD11a-, CD44+, and CD90.1+. The optimal weight ratio is 65:35 for the normalized alkaline phosphatase activities. The transduced MSCs, engineered by replication-defective adenovirus to express the BMP-2 gene, demonstrated synergic osteogenic effects in the microbeads. MSCs are capable of proliferating and differentiating in appropriate combinations of n-HA/Col. Thus it is a promising composite for future clinical applications.

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Bone Repair Analysis in a Novel Biodegradable Hydroxyapatite/Collagen Composite Implanted in Bone

Cited by 25 publications

References 22 publications

Injectable nanocrystalline hydroxyapatite paste for bone substitution: In vivo analysis of biocompatibility and vascularization

Injectable nanocrystalline hydroxyapatite paste for bone substitution: In vivo analysis of biocompatibility and vascularization

A multiscale modeling approach to scaffold design and property prediction

Characterization and osteogenic effects of mesenchymal stem cells on microbeads composed of hydroxyapatite nanoparticles/reconstituted collagen

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