Bone regeneration with resorbable polymeric membranes. III. Effect of poly(L‐lactide) membrane pore size on the bone healing process in large defects

Pineda, Leonilo M.; Büsing, Michael; Meinig, Richard P.; Gogolewski, Sylwester

doi:10.1002/(sici)1097-4636(199607)31:3<385::aid-jbm13>3.3.co;2-a

Cited by 21 publications

(30 citation statements)

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“…In this technique, the membranes are positioned on the periosteal surface to provide a structure for bone formation. It has been argued that while 3-D scaffolds support the ingrowth of cells and tissue, the 2-D membranes may also protect the defect from soft tissue ingrowth and guide cell migration from the periosteum [14, 51]. Since the membranes are placed on the periphery of the defect, they retain space for bone deposition throughout the defect.…”

Section: Discussionmentioning

confidence: 99%

“…This is not surprising, since previous studies have demonstrated the need for a biologic stimulus for effective bone regeneration in this challenging model [11-12]. A series of studies have been performed to investigate the ability of polymer membranes to heal segmental diaphyseal defects [14, 21, 51, 54]. Pineda et al implanted porous polylactide membranes thermoformed into tubes in 1-cm defects in the rabbit radius, and observed bridging with new endosteal bone generation from the native bone ends [51].…”

Section: Discussionmentioning

confidence: 99%

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An alginate-based hybrid system for growth factor delivery in the functional repair of large bone defects

Kolambkar¹,

Dupont²,

Boerckel³

et al. 2011

Biomaterials

453

360

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The treatment of challenging fractures and large osseous defects presents a formidable problem for orthopaedic surgeons. Tissue engineering/regenerative medicine approaches seek to solve this problem by delivering osteogenic signals within scaffolding biomaterials. In this study, weCorrespondence to: Robert E. Guldberg, robert.guldberg@me.gatech.edu. Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access Author ManuscriptBiomaterials. Author manuscript; available in PMC 2012 January 1. introduce a hybrid growth factor delivery system that consists of an electrospun nanofiber mesh tube for guiding bone regeneration combined with peptide-modified alginate hydrogel injected inside the tube for sustained growth factor release. We tested the ability of this system to deliver recombinant bone morphogenetic protein-2 (rhBMP-2) for the repair of critically-sized segmental bone defects in a rat model. Longitudinal μ-CT analysis and torsional testing provided quantitative assessment of bone regeneration. Our results indicate that the hybrid delivery system resulted in consistent bony bridging of the challenging bone defects. However, in the absence of rhBMP-2, the use of nanofiber mesh tube and alginate did not result in substantial bone formation. Perforations in the nanofiber mesh accelerated the rhBMP-2 mediated bone repair, and resulted in functional restoration of the regenerated bone. μ-CT based angiography indicated that perforations did not significantly affect the revascularization of defects, suggesting that some other interaction with the tissue surrounding the defect such as improved infiltration of osteoprogenitor cells contributed to the observed differences in repair. Overall, our results indicate that the hybrid alginate/nanofiber mesh system is a promising growth factor delivery strategy for the repair of challenging bone injuries.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

An alginate-based hybrid system for growth factor delivery in the functional repair of large bone defects

Kolambkar¹,

Dupont²,

Boerckel³

et al. 2011

Biomaterials

453

360

View full text Add to dashboard Cite

show abstract

“…Poresize is an important characteristic of membrane-based scaffolds as it determines the overall surface area available for cell-attachment. PLLA membranes with poresizes greater than 10 µm have previously been reported in the literature [32]. All membranes reported in this research have pore diameters in the range of about 0.5 to 5 µm (Fig.…”

Section: Discussionmentioning

confidence: 81%

“…Several devices based on the degradable lactic acid polyesters including PLLA have also been approved by the FDA for human use [27 -30]. PLLA membranes have previously been reported in literature and were shown to be biocompatible [20] and sustain cell attachment [31], as well as promote bone regeneration [32].…”

mentioning

confidence: 99%

Physical characterization of thin semi-porous poly(L-lactic acid)/poly(ethylene glycol) membranes for tissue engineering

Swaminathan

Tchao

Jonnalagadda

2007

Journal of Biomaterials Science, Polymer Edition

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This study examines physical properties of solvent-cast poly(L-lactic acid) (PLLA): poly(ethylene glycol) PEG membranes as a function of PEG molecular weight (MW) and incubation in vitro for 6 weeks. PEGs of MW 400, 1450 and 8000 were used. The morphological, thermal, mechanical and permeability properties of the membranes were studied prior to and after 3 and 6 weeks of incubation in phosphate-buffered saline (PBS) at 37 degrees C. The membranes showed a thickness of about 35+/-5 microm and were found to be semi-porous, with a non-porous surface as well as a porous surface with pore-diameters of 0.5-5 microm. The surface pore size was found to be a function of PEG MW used. All membranes were mechanically strong, with elastic moduli and tensile strength of 150-440 MPa and 7-36 MPa, respectively, all through the 6-week incubation period. The lower-MW PEGs plasticized PLLA based on high initial percent elongation; however, the effect was lost after 3 weeks of incubation in PBS. All membranes except those fabricated with PEG 8000 were impermeable for up to 6 weeks of incubation in PBS. Permeability studies showed that only PLLA:PEG 8000 membranes were permeable to methylene blue after 3 weeks of degradation.

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“…Others have suggested that pore sizes up to 200 mm in polyester membranes result in the best bone ingrowth. The size of the pore has a significant effect on its mechanical integrity and its ability to perform under functional demands [23].…”

Section: Tissue Engineering Componentsmentioning

confidence: 99%

Tissue engineering in oral and maxillofacial reconstruction

Melek

2015

Tanta Dental Journal

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The artificial generation of tissues, organs, or even more complex living organisms was throughout the history of mankind a matter of myth and dream. During the last decades this vision became feasible and has been recently introduced in clinical medicine. The interest and attention that this rapidly developing area has received are based on the vision that the growing understanding of tissue healing and the achievements of biotechnology will be of profound therapeutic relevance. Clinically, reconstructive surgery has arrived at a standard of care that allows for repair and restoration of the vast majority of tissues/organs with established techniques. The real challenge of tissue engineering in clinical treatment is the reduction of surgical morbidity by the application of biological signals or bio-artificial components cultivated from the patient's own cells, that can replace the lost body part or accomplish its repair without the need for autogenous tissue transfer. Initially, activities in this area were mainly focused on cell-based approaches aiming at the generation of tissue-like constructs by combining ex vivo expanded cell populations with various types of scaffolds. Today, the field of tissue engineering has expanded tremendously, in that not only cells and scaffolds but also growth factors, controlled release carriers, engineering of biomaterials and many other areas of basic and applied research are considered to be part of the field of tissue engineering.

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Bone regeneration with resorbable polymeric membranes. III. Effect of poly(L‐lactide) membrane pore size on the bone healing process in large defects

Cited by 21 publications

References 0 publications

An alginate-based hybrid system for growth factor delivery in the functional repair of large bone defects

An alginate-based hybrid system for growth factor delivery in the functional repair of large bone defects

Physical characterization of thin semi-porous poly(L-lactic acid)/poly(ethylene glycol) membranes for tissue engineering

Tissue engineering in oral and maxillofacial reconstruction

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