Bone Regeneration with Bone Substitutes

Katthagen, B.-D.

doi:10.1007/978-3-642-71827-4

Cited by 37 publications

(40 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…31,32 The current result of untreated 6-mm diameter defect in the cancellous bone of rabbits confirmed the previous literature data on these critical-size defects. [33][34][35][36][37][38] Recently, a noncritical-size defect smaller than 4-mm diameter and 6-mm depth, created in the distal femur of rabbits, was reported to be not totally filled with new bone after 1 year. 39 Although the untreated defects still maintained, as expected, the characteristics of nonhealed defects, a comparison of the healing rate of the current internal control defects (group 4: right side treated with FDBA þ PRP þ BMSC and left side left untreated) was made with that of external control defects, belonging to studies, where no proteins, cells, or growth factors were used, 34,38 in order to confirm that crossed influence did not occur.…”

Section: Discussionmentioning

confidence: 99%

In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet‐rich plasma, and freeze‐dried bone allografts, alone and in combination

Dallari

Fini

Stagni

et al. 2006

Journal Orthopaedic Research

156

View full text Add to dashboard Cite

The repair of confined trabecular bone defects in rabbits treated by autologous bone marrow stromal cells (BMSC), platelet-rich plasma (PRP), freeze-dried bone allografts (FDBA) alone and in combination (BMSC þ PRP; FDBA þ BMSC; FDBA þ PRP; FDBA þ PRP þ BMSC) was compared. A critical size defect was created in the distal part of the femurs of 48 adult rabbits. Histology and histomorphometry were used in the evaluation of healing at 2, 4, and 12 weeks after surgery. The healing rate (%) was calculated by measuring the residual bone defect area. Architecture of the newly formed bone was compared with that of bone at the same distal femur area of healthy rabbits. The defect healing rate was higher in PRP þ BMSC, FDBA þ PRP, FDBA þ BMSC, and FDBA þ PRP þ BMSC treatments, while lower values were achieved with PRP treatment at all experimental times. The highest bone-healing rate at 2 weeks was achieved with FDBA þ PRP þ BMSC treatment, which resulted significantly different from PRP (p < 0.05) and BMSC (p < 0.05) treatments. At 4 weeks, the bone-healing rate increased except for PRP treatment. Finally, the bone-healing rate of FDBA þ PRP, FDBA þ BMSC, and FDBA þ PRP þ BMSC was significantly higher than that of PRP at 12 weeks (p < 0.05). At 12 weeks, significant differences still existed between PRP, BMSC, and FDBA groups and normal bone (p < 0.05). These results showed that the combination of FDBA, BMSC and PRP permitted an acceleration in bone healing and bone remodeling processes. ß

show abstract

Section: Discussionmentioning

confidence: 99%

In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet‐rich plasma, and freeze‐dried bone allografts, alone and in combination

Dallari

Fini

Stagni

et al. 2006

Journal Orthopaedic Research

156

View full text Add to dashboard Cite

show abstract

“…Pyrost bone substitute is considered to be osteocompatible (Katthagen, 1986). This experiment has determined that Pyrost bone substitute not only supports osteoblast attachment but also allows proliferation of the cells.…”

Section: Discussionmentioning

confidence: 99%

“…When it is used as a bone graft substitute, bony ingrowth into the implants without any adverse reaction can be demonstrated (Katthagen, 1986). When it is implanted into bone, Pyrost can form a physicochemical bond with bone tissue (Jarcho et al, 1977).…”

mentioning

confidence: 99%

In vitro cell behavior of osteoblasts on Pyrost bone substitute

et al. 1997

View full text Add to dashboard Cite

Background: Pyrost bone substitute has been shown to be a promising orthopedic biomaterial. However, little is known about mechanisms that are responsible for the genesis and development of the bond between bone and the Pyrost bone substitute. The purpose of this study is to elucidate the in vitro cell behavior of osteoblasts on Pyrost bone substitute.Methods: By using primary culture of rat osteoblasts, the changes in cell morphology during adhesion and flattening onto the surface of Pyrost bone substitute were studied in vitro. At 1 hour, at 3 hours, and at days 1, 3, and 7 after layering, the cell behavior was observed with scanning electron microscope.Results: The processes of trypsinized osteoblast adhesion and spreading on Pyrost bone substitute consisted of 1) contact of rounded osteoblasts with the Pyrost substrate; 2) attachment of osteoblasts at point of contact; 3) centrifugal growth of filopodia; 4) flattening and spreading of the osteoblasts on the Pyrost substrate; 5) division and growth of osteoblasts; and 6) suspension of the osteoblasts across the pores by their processes.Conclusions: These results demonstrated that Pyrost can form a physicochemical bond with osteoblasts. The Pyrost bone substitute not only supports osteoblasts attachment but also allows proliferation of the osteoblasts. Anat.

show abstract

“…Se emplean como animales de experimentación 36 conejos machos albinos de la raza Nueva Zelanda, Oryctolagus cuniculi, con un peso comprendido entre 3.000 y 4.000 gramos y una edad entre 4 y 6 meses, los que se intervienen quirúrgicamente creando un defecto óseo cavitario de 6 mm de diámetro centrado en la metáfisis distal del fémur derecho (Katthagen 1986).…”

Section: Materials Y Metodosunclassified

Estudio experimental de la osteosustitución con biomateriales cerámicos formulados como cementos óseos

et al. 2007

View full text Add to dashboard Cite

SUMMARYCeramic biomaterials have interesting biological properties that can be used in bone substitution. In this study two calcium phosphate bone cements were compared to cancellous bone autograft. A bone cavitary defect of 6 mm in diameter was carried out in the right distal femoral methaphyses of 36 male rabbits Oryctolagus cuniculi. The animals were divided into 3 groups of 12 rabbits receiving α tricalcium phosphate cement (TPC), monocalcium phosphate cement (MPC) and autologous bone (CON) as implants. The radiological and histological studies showed a correct substitution of both biomaterials with new bone. The histomorphometric study revealed that the bone neoformation obtained with the two cements at 4 and 12 weeks is equivalent to the bone generated by the bone graft. There were no significant differences in the reabsorption of the materials. Finally, this work concluded that the calcium phosphate bone cements are osteoconductive, osteotransductive and biocompatible materials which behave as bone substitutes.Palabras clave: fosfatos de calcio, cementos óseos. INTRODUCCIONLa pérdida de tejido óseo es un evento frecuente en la traumatología y ortopedia, tanto humana como veterinaria, de tal manera que el implante preferido para el tratamiento de las soluciones de continuidad del hueso corresponde al propio tejido óseo, ya sea proveniente del mismo, o de otro individuo. El autoinjerto esponjoso es probadamente el mejor sustitutivo óseo en la clínica humana, sin embargo, tiene como limitantes la reducida cantidad de tejido disponible y la potencial morbilidad de los sitios dadores (Arrington y col 1996, Goulet y col 1997). Alternativamente, es posible disponer del aloinjerto óseo, ya sea congelado, criopreservado o liofilizado; pero, para su obtención y utilización, se requiere contar con un banco de tejidos y un número apropiado de donantes. Por lo demás, su empleo siempre conlleva el riesgo potencial de transmisión de enfermedades al receptor (Berrey y col 1990, Segur y col 1998).En los grandes defectos óseos diafisarios se puede practicar la técnica del transporte óseo, que a través de la osteogénesis por distracción es capaz de resolver exitosamente amplias pérdidas tisulares óseas (Ilizarov 1989). También es posible utilizar injertos óseos vascularizados que consiguen el mismo fin aprovechando las técnicas microquirúrgicas (Taylor y col 1975). No obstante, ninguno de estos dos métodos es aplicable para tratar defectos cavitarios.Debido a las conocidas limitaciones que ostentan los injertos óseos, numerosos grupos de investigadores han estudiado variados biomateriales que pueden tener utilidad como sustitutivos óseos, sobresaliendo especialmente las cerámicas de fosfato de calcio, dado que detentan reconocidas propiedades osteoconductivas y son capaces de otorgar un andamiaje que en forma progresiva es sustituido por tejido óseo diferenciado (Hench 1998, LeGeros 2002. A este grupo pertenecen los cementos óseos basados en fosfatos de calcio.La formulación de los biomateriales cerámicos como cementos óse...

show abstract

Bone Regeneration with Bone Substitutes

Cited by 37 publications

References 0 publications

In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet‐rich plasma, and freeze‐dried bone allografts, alone and in combination

In vivo study on the healing of bone defects treated with bone marrow stromal cells, platelet‐rich plasma, and freeze‐dried bone allografts, alone and in combination

In vitro cell behavior of osteoblasts on Pyrost bone substitute

Estudio experimental de la osteosustitución con biomateriales cerámicos formulados como cementos óseos

Contact Info

Product

Resources

About