Bone Regeneration Using an Acellular Extracellular Matrix and Bone Marrow Mesenchymal Stem Cells Expressing Cbfa1

Preparation of poly(ethylene glycol)/polylactide hybrid fibrous scaffolds for bone tissue engineering

“…As the traditional treatments for bone lesions, autologous bone grafts are restricted because of donor site morbidity and potential secondary wounds, while allogeneic bone grafts are limited because of the risk of potential transmission of pathogens. 1 As a consequence, various bone substitutes with similar structure and function to autologous bones have been proposed. As temporary templates for cell seeding, migration, proliferation, and differentiation prior to the regeneration of natural extracellular matrix (ECM) or biological functional tissue, 2,3 biomaterial scaffolds for bone tissue engineering applications require consideration of proper toughness for cell adhesion, porous three-dimensional structure for cell migration and new bone tissue ingrowth, as well as good osteoconductivity to promote bone repair.…”

“…33,34 MSCs isolated from bone marrow are multipotent cells that can be induced to differentiate into a variety of mesenchymal tissues including bone, cartilage, tendon, fat, and muscle both in vitro and in vivo. 1 MSCs have been widely applied in tissue engineering because they are easy to get and manipulate, can be easily differentiated, are biocompatible almost without immune response or tumorigenesis, and especially because they pose no ethical or legal problems. 35 Therefore, MSCs are well suited to act as seed cells for successful tissue renewal and replacement of damaged and diseased musculoskeletal tissues in bone tissue engineering.…”

Preparation and characterization of polylactide/poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) hybrid fibers for potential application in bone tissue engineering

“…Figure 2A shows the synthesis scheme of copolymer PCEC. GPC results ( Figure 2B) show that M n and M w of PCEC were 2.24×10 4 and 4.61×10 4 , respectively. Only one single peak existed, which indicated the mono-distribution of macromolecular weight and the absence of any homopolymer of ε-CL or PEG, and also implied that no transesterification or backbiting reactions occurred during polymerization.…”

“…3 Available quantities are limited, however, and the harvesting procedure is burdened by comorbidities. 4,5 With an increasing demand for and decreasing supply of traditional bone graft tissue, tissue engineering techniques are being developed and applied in clinical use as alternatives. [6][7][8][9] Recently, combined cells with fibrous scaffolds have been shown to be an effective way to treat bone defects and promote bone regeneration.…”

rFN/Cad-11-Modified Collagen Type II Biomimetic Interface Promotes the Adhesion and Chondrogenic Differentiation of Mesenchymal Stem Cells

“…37 In brief, MSCs were isolated from the femurs of 4-week-old Japanese white rabbits (1.5-2.0 kg; Animal Research Center, Third Military Medical University). After anesthesia, rabbit bone marrow was collected into a 10-mL syringe that contained 5000 U of heparin.…”