Bone regeneration in segmental defects with resorbable polymeric membranes: IV. Does the polymer chemical composition affect the healing process?

Gogolewski, Sylwester; Pineda, Leonilo M.; Büsing, C. M.

doi:10.1016/s0142-9612(00)00119-8

Cited by 47 publications

(31 citation statements)

References 24 publications

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“…Its size makes it convenient to implement operative procedures. The rabbit bone defect model, such as circular calvarial bone defect or segmental radial bone defect, has been widely applied during the study of bone defect repair [Turk et al, 1993;An et al, 1999;Gogolewski et al, 2000;Wang et al, 2002;Jung et al, 2006]. Thus, the osteogenesis of ASCs should be sufficiently determined in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

Collagen I Gel Can Facilitate Homogenous Bone Formation of Adipose-Derived Stem Cells in PLGA-β-TCP Scaffold

Wei

et al. 2007

Cells Tissues Organs

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Cell-based tissue engineering is thought to be a new therapy for treatment of bone defects and nonunions after trauma and tumor resection. In this study, we explore the in vitro and in vivo osteogenesis of a novel biomimetic construct fabricated by using collagen I gel to suspend rabbit adipose-derived stem cells (rASCs) into a porous poly(lactic-co-glycolic)acid-β-tricalcium phosphate (PLGA-β-TCP) scaffold (rASCs-COL/PLGA-β-TCP). In vitro and in vivo studies of the rASCs-COL/PLGA-β-TCP composite (group A) were carried out compared with the single combination of rASCs and PLGA-β-TCP (rASCs/PLGA-β-TCP; group B), the combination of acellular collagen I gel and PLGA-β-TCP (COL/PLGA-β-TCP; group C), and the PLGA-β-TCP scaffold (group D). Composites of different groups were cultured in vitro for 2 weeks in osteogenic medium and then implanted into the autologous muscular intervals for 8 weeks. After 2 weeks of in vitro culture, alkaline phosphatase activity and extracellular matrix mineralization in group A were significantly higher than in group B (p < 0.01, n = 4). In vivo osteogenesis was evaluated by radiographic and histological analyses. The calcification level was radiographically evident in group A, whereas no apparent calcification was observed in groups B, C and D (n = 4). In group A, woven bone with a trabecular structure was formed, while in group B, only osteoid tissue was observed. Meanwhile, the bone-forming area in group A was significantly higher than in group B (p < 0.01, n = 4). No bone formation was observed in groups C or D (n = 4). In conclusion, by using collagen I gel to suspend rASCs into porous PLGA-β-TCP scaffold, osteogenic differentiation of rASCs can be improved and homogeneous bone tissue can be successfully formed in vivo.

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Section: Introductionmentioning

confidence: 99%

Collagen I Gel Can Facilitate Homogenous Bone Formation of Adipose-Derived Stem Cells in PLGA-β-TCP Scaffold

Wei

et al. 2007

Cells Tissues Organs

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“…Entre os polímeros mais utilizados na recuperação de fraturas ósseas, se encontram os poli( -hidróxi ácidos) e seus copolímeros, sendo possível a obtenção de materiais com diferentes propriedades mecânicas, e diferentes taxas de degradação, de acordo com as proporções de monômeros usadas nas sínteses destes copolímeros [10][11][12][13][14] . O processo de biorreabsorção desses materiais ocorre através da hidrólise de suas ligações ésteres em contato com os fluidos corpóreos, originando produtos na forma de oligômeros solúveis e não tóxicos, que após sofrerem a ação metabólica do organismo são transformados em CO 2 e H 2 O [15] .…”

Section: Introductionunclassified

Síntese e caracterização do copolímero poli (L-co-D,L Ácido Láctico)

Motta

Duek²

2007

Polímeros

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Resumo: A aplicação de polímeros biorreabsorvíveis em próteses temporárias é constante nos procedimentos médicos relacionados a fraturas ósseas. Dentre os polímeros bioreabsorvíveis, o poli(L-co-D, L ácido láctico), PLDLA, na relação 70:30, tem sido estudado visando à obtenção de placas e parafusos para a recuperação de traumas nas regiões buco e crânio-maxilofacial. Nessa relação de monômeros obtém-se um polímero amorfo, o que permite uma adaptação do dispositivo ao local do implante durante a cirurgia. Um fator limitante para o uso desse polímero é seu alto custo em função da importação. Neste trabalho o PLDLA foi sintetizado através da polimerização em massa dos monômeros cíclicos do L-ácido láctico e do D, L ácido láctico, utilizando como catalisador o Sn(Oct) 2 . Obteve-se material de alta massa molar (Mw = 10 5 g/mol), o qual foi caracterizado por 1 H RMN, 13 C RMN, GPC, FTIR e DSC. Palavras-chave: Poli(L-co-D,L ácido lático), síntese, caracterização. Synthesis and Characterization of the Copolymer Poly(L-co-D,L lactic acid)Abstract: Bioreabsorbable polymers are routinely used as temporary prostheses for fractured bones. Among the bioreabsorbable polymers the poly(L-co-D, L lactic acid), PLDLA, in the 70:30 rate has been studied to obtain plates and screws to recuperate traumas in the cranium and maxilla facial regions. In this monomers rate, an amorphous polymer is obtained, which allows adaptation of devices in the local for the implant during the surgery. A limiting factor to use this polymer is its high cost, due to the importation process. In this work PLDLA was synthesized by mass polymerization of the cyclical monomers L-lactic acid and D, L lactic acid, using Sn(Oct) 2 as catalyst. A material with high molar mass (10 5 g/mol) was obtained and characterized by 1 H RMN, 13 C RMN, GPC, FTIR and DSC. Keywords: Poly(L-co-D,L lactic acid), synthesis, characterization. IntroduçãoDispositivos obtidos a partir de polímeros biorreabsorvíveis aplicados em osteofixação ao têm sido cada vez mais aceitos na área de ortopedia e traumatologia [1] . Atualmente, dispositivos como mini placas e parafusos desses polímeros são usados rotineiramente na região crânio e bucomaxilofacial, substituindo os dispositivos metálicos [2] .A utilização de materiais permanentes, como o titânio, na fixação de fraturas já data de muitas décadas. Apesar da elevada propriedade mecânica que tais implantes apresentam, o grande inconveniente de sua utilização reside nos elevados percentuais de remoção destes dispositivos do organismo, após a consolidação da fratura. Um exemplo que descreve a necessidade de remoção dos implantes metálicos no organismo pode ser dado quando o implante é aplicado a fraturas na região craniofacial em crianças, tendo em vista que a não-remoção do mesmo, implica num comprometimento do crescimento ósseo destes indivíduos, além da possibilidade de migração destes implantes [2][3][4][5][6][7] . Pesquisa recente [8] mostrou que para a maioria dos pacientes, 91%, que possuem algum tipo de implante metálico, o aspecto...

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“…2 In this respect, the application of synthetic membranes recently has been considered to be very prospective, as their chemical composition and mechanical properties can be easily modified according to specific needs. [3][4][5][6] The first tissueengineered organ, processed from the lab for the first accepted patients' care, was skin. 7 Currently, there is a huge number of patients with deep burns or nonhealing skin ulcers, and many of them would benefit from a large-scale production of bioartificial skin.…”

Section: Introductionmentioning

confidence: 99%

Interaction of human skin fibroblasts with moderate wettable polyacrylonitrile–copolymer membranes

Groth¹,

Seifert²,

Malsch³

et al. 2002

J. Biomed. Mater. Res.

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The development of a bioartificial skin is a step toward the treatment of patients with deep burns or nonhealing skin ulcers. One possible approach is based on growing dermal cells on membranes to obtain appropriate living cellular stroma (sheets) to cover the wound. New membrane-forming copolymers were synthesized, based on acrylonitrile (AN) copolymerization with hydrophilic N-vinylpyrrolidone (NVP) monomer, in different percentage ratios, such as 5, 20, and 30% w/w, and with two other relatively high polar comonomers--namely, sodium 2-methyl-2-propene-1-sulfonic acid (NaMAS) and aminoethylmethacrylate (AeMA). All these copolymers were characterized for their bulk composition and number average molecular weight, and used to prepare ultrafiltration membranes. Water contact angles and water uptake were estimated to characterize the wettability and scanning force microscopy to visualize the morphology of the resulting polymer surface. Cytotoxicity was estimated according to the international standard regulations, and the materials were found to be nontoxic. The interaction of the membranes with human skin fibroblasts was investigated considering that these cells are among the first to colonize membranes upon implantation or with prolonged external contact. The overall cell morphology, formation of focal adhesion contacts, and cell proliferation were estimated to characterize the cell material interactions. It was found that the pure polyacrylonitrile homopolymer (PAN) membrane provides excellent conditions for seeding with fibroblasts, comparable only to a copolymer containing AeMA. In contrast, the presence of NaMAS with acidic ionic groups decreased both the attachment and proliferation of fibroblasts. Low content of NVP in the copolymer, up to about 5%, still enabled good attachment and spreading of cells, as well as subsequent proliferation of fibroblasts, but higher ratios of 20 and 30% resulted in a significant decrease of these cellular activities.

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Bone regeneration in segmental defects with resorbable polymeric membranes: IV. Does the polymer chemical composition affect the healing process?

Cited by 47 publications

References 24 publications

Collagen I Gel Can Facilitate Homogenous Bone Formation of Adipose-Derived Stem Cells in PLGA-β-TCP Scaffold

Collagen I Gel Can Facilitate Homogenous Bone Formation of Adipose-Derived Stem Cells in PLGA-β-TCP Scaffold

Síntese e caracterização do copolímero poli (L-co-D,L Ácido Láctico)

Interaction of human skin fibroblasts with moderate wettable polyacrylonitrile–copolymer membranes

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