Bone regeneration in rabbit calvarial critical‐sized defects filled with composite in situ formed xenogenic dentin and biphasic tricalcium phosphate/hyroxyapatite mixture

Abstract

The purpose of this study was to evaluate bone healing in calvarial defects using two bone graft substitute materials; biphasic beta‐tricalcium phosphate/hydroxyapatite in hydrogel (ß‐TCP/HA) versus composite non‐demineralized xenogenic dentin with ß‐TCP/HA mixture. Full thickness critical‐sized defects were created bilaterally in 10 New Zealand male rabbits. Seven defects were left empty, six filled with biphasic tricalcium phosphate putty, and seven were filled with composite non‐demineralized xenogenic dentin with biphasic tricalcium phosphate. Animals were sacrificed at eight weeks postoperatively and the healing of the biomaterial‐filled defects was compared radiographically and by histomorphometry. Micro‐computed tomography (μCT) was utilized to analyze the osteogenesis and healing patterns of the defects. Quantitative analysis of volume fraction (%) of the newly formed bone and remaining graft material (FV=filling volume/TV=tissue volume) and mean intensity [HU] in the defects were evaluated. Defects filled with composite dentin with biphasic tri‐calcium phosphate showed volume fraction (FV/TV) in the order of 55.81% ± 17.72%, whereas defects filled with only biphasic tricalcium phosphate showed a fraction of 39.84% ± 16.06%, which represent the ratio of remaining graft material and new bone formation to the tissue volume. The empty negative control defects showed a volume fraction of 19.14% ± 8.787%. Histological analysis showed significant percentage increase in bone formation and residual graft with the composite Dentin/ß‐TCP group after 8 weeks. The findings suggest that composite xenogenic dentin with biphasic tricalcium phosphate showed improved osteogenesis when compared to biphasic tricalcium phosphate without the addition of non‐demineralized dentin. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 773–782, 2019.

In Vivo Evaluation of the Regenerative Capability of Glycylglycine Ethyl Ester-Substituted Polyphosphazene and Poly(lactic-co-glycolic acid) Blends: A Rabbit Critical-Sized Bone Defect Model

“…X-ray analyses were used to examine new bone formation around the defect region as the X-ray images of the rabbits’ forelegs were obtained at each time point and for each matrix. The images showed a progressive increase in the radiodensity (or radiopacity) around the defect areas, thought to be consistent with new bone formation and growth . The earliest time point (second week) results showed increased radiodensity around the edges of the precreated defects.…”

“…The images showed a progressive increase in the radiodensity (or radiopacity) around the defect areas, thought to be consistent with new bone formation and growth. 31 The earliest time point (second week) results showed increased radiodensity around the edges of the precreated defects. The process continued with more areas of the defects, demonstrating increased bone formation after 4 weeks, and this is indicated on the X-ray images shown in Figure 8.…”

In Vivo Evaluation of Regenerative Osteogenic Potential Using a Human Demineralized Dentin Matrix for Dental Application

“…Previous studies tried to test the osteoinductive potential of an un-demineralized dentin matrix on bone formation [ 27 ]. However, using a rat model, Mordenfeld et al assessed how demineralization of dentin particles affected the growth of bone in an osteoconductive environment.…”

Обоснование Применения Остеопластических Материалов При Костно-Деструктивных Изменениях Альвеолярного Отростка В Хирургической Стоматологии

“…Остеопластические материалы должны быть остеогенными, что обеспечивает рост костной ткани за счет остеогенных клеток, остеоиндуктивными, что обеспечивает рост ткани путем дифференцировки мезенхимальных стволовых клеток, остеокондуктивными для обеспечения матрикса новой ткани, также остеопластические материалы должны обладать высокой пористостью для обеспечения физиологического ангиогенеза, биосовместимостью, а также стерильностью [3,4].…”

“…Преимущества данных материалов по определенным параметрам проявляются в отсутствии забора костной ткани, вероятности инфицирования. Положительным аспектом использования синтетических остеопластических материалов является отсутствие ограничений по количеству, простота хранения, стерильность и резорбируемость материалов, стабильность химического состава и формы, отсутствие аллергических реакций, а недостатком группы синтетических материалов является отсутствие остеоиндуктивных свойств, неконтролируемые сроки рассасывания (до 3-х лет), при этом рассасывание материала в ряде случаев может не сопровождаться формированием костной ткани [1,3].…”