Bone regeneration following injection of mesenchymal stem cells and fibrin glue with a biodegradable scaffold

Yamada, Yoichi; Boo, Jae Seong; Ozawa, Ryotaro; Nagasaka, Tetsuro; Okazaki, Yoshimitsu; Hata, Ken‐ichiro; Ueda, Minoru

doi:10.1016/s1010-5182(02)00143-9

Cited by 216 publications

(146 citation statements)

References 26 publications

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“…[1][2][3][4][5][6][7][8][9] An emerging group of successful research efforts have focused on exploiting this condition over the past decade. These recent publications (Tables 2-4) have demonstrated the capacity of cartilage tissue constructs to promote EC ossification in vivo after in vitro priming with seeded BMSCs, 60,63,64,66,67,[72][73][74][75][76][103][104][105][106][107] ESCs, 27,62,67,76 ADSCs, 108 iPSCs, 109 and articular chondrocytes (ACs). 61,67,70,71,75 Most of these studies utilized a heterotopic bone formation model in immunocompromised animals to provide evidence of osteo-and chondro-conductivity and inductivity.…”

Section: Incorporating Scbts Into the Designmentioning

confidence: 99%

Endochondral Ossification for Enhancing Bone Regeneration: Converging Native Extracellular Matrix Biomaterials and Developmental Engineering In Vivo

Dennis

Berkland

Bonewald

et al. 2015

Tissue Engineering Part B: Reviews

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Section: Incorporating Scbts Into the Designmentioning

confidence: 99%

Endochondral Ossification for Enhancing Bone Regeneration: Converging Native Extracellular Matrix Biomaterials and Developmental Engineering In Vivo

Dennis

Berkland

Bonewald

et al. 2015

Tissue Engineering Part B: Reviews

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“…To test the possibility of employing fibrin based gels for injectable purposes, Yamada et al used a combination of fibrin gel with rat MSCs and β-TCP scaffolds to generate bone at an ectopic site [25]. In a follow up study, they replaced the fibrin gel with platelet rich plasma (PRP) gel (PRP gel is a fibrin gel enriched with the growth factors released from platelets) with in vitro expanded canine MSCs for treating mandibular defects in dogs [26].…”

Section: Page 4 Of 35mentioning

confidence: 99%

Engineering New Bone via a Minimally Invasive Route Using Human Bone Marrow-Derived Stromal Cell Aggregates, Microceramic Particles, and Human Platelet-Rich Plasma Gel

Chatterjea

Yuan

Chatterjea

et al. 2013

Tissue Engineering Part A

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Tissue Engineering Part A eering new bone via a minimally invasive route using human bone marrow derived stromal cell aggregates, micro ceramic particles and human platelet rich plasma gel (doi: 10.1089(doi: 10. /ten.TEA.2012 This article has been peer-reviewed and accepted for publication, but has yet to undergo copyediting and proof correction. The final published version may differ from this proof. 2 AbstractThere is a rise in the popularity of arthroscopic procedures in orthopedics. However, the majority of cell based bone tissue engineered constructs rely on solid pre-formed scaffolding materials, which require large incisions and extensive dissections for placement at the defect site. Thus, they are not suitable for minimally invasive techniques.The aim of this study was to develop a clinically relevant, easily moldable, bone tissue engineered construct (TEC), amenable to minimally invasive techniques, using human mesenchymal stromal cells (hMSC) and calcium phosphate micro particles in combination with an in-situ forming platelet rich plasma (PRP) gel obtained from human platelets. Most conventional TECs rely on seeding and culturing single cell suspensions of hMSCs on scaffolds. However, for generating TECs amenable to the minimally invasive approach, it was essential to aggregate the hMSCs in vitro prior to seeding them on the scaffolds as unaggregated MSCs did not generate any bone. 24 hours of in vitro aggregation was determined to be optimal for maintaining cell viability in vitro and bone formation in vivo. Moreover, no statistically significant difference was observed in the amount of bone formed when the TECs were implanted via an open approach or a minimally invasive route. TECs generated using MSCs from three different human donors generated new bone through the minimally invasive route in a reproducible manner, suggesting that these TECs could be a viable alternative to pre-formed scaffoldsemployed through an open surgery for treating bone defects. Page 2 of 35Tissue Engineering Part A eering new bone via a minimally invasive route using human bone marrow derived stromal cell aggregates, micro ceramic particles and human platelet rich plasma gel

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“…The new technology 'Tissue Engineered Bone (TEB)' that we developed is so called '"injectable bone," 13,14) and involves the morphogenesis of new tissue using constructs formed from isolated cells with biocompatible scaffolds and growth factors.…”

Section: Figmentioning

confidence: 99%

Tissue engineered bone; Application for implant surgery

Ueda

2011

Inflammation and Regeneration

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Bone regeneration following injection of mesenchymal stem cells and fibrin glue with a biodegradable scaffold

Cited by 216 publications

References 26 publications

Endochondral Ossification for Enhancing Bone Regeneration: Converging Native Extracellular Matrix Biomaterials and Developmental Engineering In Vivo

Endochondral Ossification for Enhancing Bone Regeneration: Converging Native Extracellular Matrix Biomaterials and Developmental Engineering In Vivo

Engineering New Bone via a Minimally Invasive Route Using Human Bone Marrow-Derived Stromal Cell Aggregates, Microceramic Particles, and Human Platelet-Rich Plasma Gel

Tissue engineered bone; Application for implant surgery

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