Bone morphogenetic proteins (BMP) 2, 4, 6 and 7 affect ovarian follicular development through regulation of follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) expression in goat granulosa cells

Zhu, G.Q.

doi:10.5897/jcbg12.004

Cited by 10 publications

(6 citation statements)

References 36 publications

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“…In vitro, BMP6 expression did not exhibit a specific manner, similarly to AMH, AMHR-II, and BMP2 expressions observed in in vitro culturing of GCs from 3-8 mm follicles. Unlike BMP2, BMP6 does not support FSH stimulation [56,57]. Our results also depict a varying expression trend of BMP2 and BMP6 in vitro.…”

Section: Discussioncontrasting

confidence: 52%

“…In bovines, the concentration of AMH was decreased with an increasing size of follicle, while a difference in transcript level of GC is not always parallel with the hormone concentration of follicular fluid [10,36]. AMH inhibits FSH-dependent follicle growth and estrogen production in follicles, while BMP6 has the opposite effect [56,57]. As per the above discussion, BMPs play an important role at the FSH-AMH nexus in bovine GCs to modulate folliculogenesis [48,55].…”

Section: Discussionmentioning

confidence: 79%

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Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones

Umer

Sammad

Zou

et al. 2019

Genes

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Anti-Mullerian hormone (AMH) is an important reproductive marker of ovarian reserve produced by granulosa cells (GCs) of pre-antral and early-antral ovarian follicles in several species, including cattle. This hormone plays a vital role during the recruitment of primordial follicles and follicle stimulating hormone (FSH)-dependent follicular growth. However, the regulatory mechanism of AMH expression in follicles is still unclear. In this study, we compared the expression of AMH, AMHR-II, BMP2, BMP6, FSHR, and LHCGR genes during follicular development. In-vitro expression study was performed with and without FSH for AMH, AMHR-II, BMP2, and BMP6 genes in bovine GCs which were isolated from 3-8 mm follicles. Association among the mRNA expression and hormone level was estimated. GCs were collected from small (3-8 mm), medium (9-12 mm) and large size (13 to 24 mm) follicles before, during onset, and after deviation, respectively. Further, mRNA expression, hormones (AMH, FSH, and LH), apoptosis of GCs, and cell viability were detected by qRT-PCR, ELISA, flow cytometry, and spectrophotometry. AMH, AMHR-II, BMP2, and FSHR genes were highly expressed in small and medium follicles as compared to large ones. In addition, the highest level of AMH protein (84.14 ± 5.41 ng/mL) was found in medium-size follicles. Lower doses of FSH increased the viability of bovine GCs while higher doses repressed them. In-vitro cultured GCs treated with FSH significantly increased the AMH, AMHR-II, and BMP2 expression levels at lower doses, while expression levels decreased at higher doses. We found an optimum level of FSH (25 ng/mL) which can significantly enhance AMH and BMP2 abundance (p < 0.05). In summary, AMH, AMHR-II, and BMP2 genes showed a higher expression in follicles developed in the presence of FSH. However, lower doses of FSH demonstrated a stimulatory effect on AMH and BMP2 expression, while expression started to decline at the maximum dose. In this study, we have provided a better understanding of the mechanisms regulating AMH, AMHR II, and BMP2 signaling in GCs during folliculogenesis, which would improve the outcomes of conventional assisted reproductive technologies (ARTs), such as superovulation and oestrus synchronization in bovines.

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Section: Discussioncontrasting

confidence: 52%

Section: Discussionmentioning

confidence: 79%

Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones

Umer

Sammad

Zou

et al. 2019

Genes

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“…Estrogen directly inhibits FasL-induced apoptosis via the estrogen receptor, which activates the Akt–dependent pathway ( 20 ). In addition, the BMP ligands have been shown to inhibit apoptosis signaling down-stream of these signaling pathways in a number of studies ( 25 , 35 – 38 ).…”

Section: Major Apoptosis Signaling Pathways In the Ovarian Folliclementioning

confidence: 99%

“…In vitro culture of caprine granulosa cells with BMP2, 4, 6, and 7 reduces the percentage of DNA fragmentation ( 38 ). In the same study, FSHR silencing increased apoptosis.…”

Section: Mitochondrial Pathways Of Apoptosis and The Bmpsmentioning

confidence: 99%

Granulosa Cell Apoptosis in the Ovarian Follicle—A Changing View

et al. 2018

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Recent studies challenge the previous view that apoptosis within the granulosa cells of the maturing ovarian follicle is a reflection of aging and consequently a marker for poor quality of the contained oocyte. On the contrary, apoptosis within the granulosa cells is an integral part of normal development and has limited predictive capability regarding oocyte quality or the ensuing pregnancy rate in in vitro fertilization programs. This review article covers our revised understanding of the process of apoptosis within the ovarian follicle, its three phenotypes, the major signaling pathways underlying apoptosis as well as the associated mitochondrial pathways.

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“…Moreover, the BMP2, 4, and 7 ligands in the WT goat and human have been shown to increase the level of FSHR mRNA, whereas BMP6 was shown to increase LHR mRNA, which indicates a direct role for BMPs in the regulation of receptor density (Ogura Nose et al 2012, Zhu et al 2013. Ovine granulosa cells cultured with both FSH and estrogen increased the expression of BMPR1B (Chen et al 2009), which supports the present findings of increased BMPR1B density in the growing subordinate follicles and the reduced BMPR1B activity observed in the down-regulation of FSHR and BMPR1B in preparation for ovulation of the dominant follicle (Chen et al 2009).…”

Section: Gonadotropin Regulation and Fshr Signalingmentioning

confidence: 99%

Flow cytometric analysis of FSHR, BMRR1B, LHR and apoptosis in granulosa cells and ovulation rate in merino sheep

Regan¹,

McFarlane²,

O’Shea³

et al. 2015

Reproduction

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The aim of the present study was to determine the direct cause of the mutation-induced, increased ovulation rate in Booroola Merino (BB) sheep. Granulosa cells were removed from antral follicles before ovulation and post-ovulation from BB (nZ5) and WT (nZ12) Merino ewes. Direct immunofluorescence measurement of mature cell surface receptors using flow cytometry demonstrated a significant up-regulation of FSH receptor (FSHR), transforming growth factor beta type 1, bone morphogenetic protein receptor (BMPR1B), and LH receptor (LHR) in BB sheep. The increased density of FSHR and LHR provide novel evidence of a mechanism for increasing the number of follicles that are recruited during dominant follicle selection. The compounding increase in receptors with increasing follicle size maintained the multiple follicles and reduced the apoptosis, which contributed to a high ovulation rate in BB sheep. In addition, we report a mutation-independent mechanism of down-regulation to reduce receptor density of the leading dominant follicle in sheep. The suppression of receptor density coincides with the cessation of mitogenic growth and steroidogenic differentiation as part of the luteinization of the follicle. The BB mutation-induced attenuation of BMPR1B signaling led to an increased density of the FSHR and LHR and a concurrent reduction in apoptosis to increase the ovulation rate. The role of BMPs in receptor modulation is implicated in the development of multiple ovulations.Reproduction (2015) 150 151-163

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Bone morphogenetic proteins (BMP) 2, 4, 6 and 7 affect ovarian follicular development through regulation of follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) expression in goat granulosa cells

Cited by 10 publications

References 36 publications

Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones

Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones

Granulosa Cell Apoptosis in the Ovarian Follicle—A Changing View

Flow cytometric analysis of FSHR, BMRR1B, LHR and apoptosis in granulosa cells and ovulation rate in merino sheep

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