Bone morphogenetic protein‐7 incorporated polycaprolactone scaffold has a great potential to improve survival and proliferation rate of the human embryonic kidney cells

Regeneration and restoring the function of the myocardial‐infracted hearts have been one of the constant challenges in medicine. Recently, tissue engineering, using biocompatible substrates and stem cells, holds a real promise to solve these problems. Herein, poly(lactic‐co‐glycolic acid) (PLGA) nanofibers and platelet‐rich plasma (PRP) enriched PLGA nanofibers (PLGA‐PRP) were fabricated by electrospinning. Scanning electron microscopy (SEM) demonstrated that fiber diameters in PLGA scaffolds with and without PRP were in the range of 500 ± 280 nm and fibers were also bead free, smooth, in random orientation, and with interconnected pores. During culture of the human‐induced pluripotent stem cells (iPSCs) on the nanofibrous scaffold, further differentiation of the iPSCs to cardiomyocytes was detected in PLGA‐PRP nanofibers compared to the PLGA. This improvement in differentiation potential was evaluated at the morphological, molecular gene, and protein expression levels using SEM, real‐time reverse transcription‐polymerase chain reaction (RT‐PCR), and immunocytochemistry, respectively. The results obtained in this study highlighted the significance of natural growth factors present in the artificial scaffold applied in cardiac tissue engineering according to the improvements in cell‐biomaterial interactions. Taken together, our result indicated that PRP‐incorporated PLGA could be considered as a great potential candidate to use for engineering suitable myocardium replacement constructs.

Section: Methodsmentioning

confidence: 99%

Efficient cardiomyocyte differentiation of induced pluripotent stem cells on PLGA nanofibers enriched by platelet‐rich plasma

Torabi

Abazari

Karizi

et al. 2020

“…Release profile of the BMP2 was measured based on the previously reported method. 23 The scaffolds containing BMP2 were cut into a certain dimension and weight and then rinsed in PBS (10 mM, pH 7.4) at 37 C while gently shake at 50 RPM. A 500μL of the solution was removed in several time points and replaced with the same volume of the fresh PBS.…”

Section: Release Profilementioning

confidence: 99%

Acceleration of osteogenic differentiation by sustained release of BMP2 in PLLA/graphene oxide nanofibrous scaffold

Abazari

Nasiri

Nejati

et al. 2020

After about three decades of experience, tissue engineering has become one of the most important approaches in reconstructive medical research to treat non-selfhealing bone injuries and lesions. Herein, nanofibrous composite scaffolds fabricated by electrospinning, which containing of poly(L-lactic acid) (PLLA), graphene oxide (GO), and bone morphogenetic protein 2 (BMP2) for bone tissue engineering applications. After structural evaluations, adipose tissue derived mesenchymal stem cells (AT-MSCs) were applied to monitor scaffold's biological behavior and osteoinductivity properties. All fabricated scaffolds had nanofibrous structure with interconnected pores, bead free, and well mechanical properties. But the best biological behavior including cell attachment, protein adsorption, and support cells proliferation was detected by PLLA-GO-BMP2 nanofibrous scaffold compared to the PLLA and PLLA-GO. Moreover, detected ALP activity, calcium content and expression level of bone-related gene markers in AT-MSCs grown on PLLA-GO-BMP2 nanofibrous scaffold was also significantly promoted in compression with the cells grown on other scaffolds. In fact, the simultaneous presence of two factors, GO and BMP2, in the PLLA nanofibrous scaffold structure has a synergistic effect and therefore has a promising potential for tissue engineering applications in the repair of bone lesions.

“…The tendency of the constructed scaffolds to absorb proteins was determined according to the previously reported method 23 . Briefly, three solutions were prepared including: solution (a): phosphate buffered saline (PBS) and fetal bovine serum (FBS) (l% v/v), solution, (b): PBS and 2.0 wt% sodium dodecylsulfate (SDS), and solution, and (c): BSA in 2.0 wt% SDS in PBS.…”

Section: Methodsmentioning

confidence: 99%

Different osteoconductivity of PLLA/PHB composite nanofibers prepared by one‐ and two‐nozzle electrospinning

Sabouri

Rezaie²,

Enderami

et al. 2021

Self Cite

Electrospinning is a method that has a high potential for use in tissue engineering due to its simplicity, cheapness, versatility, and high applicability for preparing of composite nanofibrous scaffolds. In this study, nanofibrous scaffolds were made of poly‐l‐lactic acid (PLLA) and Polyhydroxybutyrate (PHB) polymers by electrospinning single‐nozzle (combined polymers: C‐PLLA/PHB) and two‐nozzle (separated polymers: S‐PLLA/PHB). Constructed scaffolds were characterized using SEM, in vitro degradation, cell attachment, protein adsorption, and nontoxicity assays. Then, scaffolds osteoconductive capacity was investigated by culturing adipose‐derived stem cells (ADSCs) and by evaluating common osteogenic markers. Obtained results displayed that fibers diameter, scaffold degradation, and biocompatibility of S‐PLLA/PHB nanofibrous scaffold were increased compared to the C‐PLLA/PHB nanofibers. In addition, significantly increased alkaline phosphatase (ALP) activity, calcium content, and osteogenic‐related gene expression were also observed in the ADSCs cultured on S‐PLLA/PHB nanofibrous scaffold compared to the cultured cells on C‐PLLA/PHB nanofibrous scaffold. Overall, this study shows that using two‐nozzle electrospinning to constructing PLLA/PHB nanofibrous scaffold can improve its osteoconductive capacity, and also its combination with ADSCs can be a promising candidate to use in bone tissue engineering.