Bone morphogenetic protein-5 (BMP-5) promotes dendritic growth in cultured sympathetic neurons

Beck, Hiroko Nagasawa; Drahushuk, Karen M.; Jacoby, David B.; Higgins, Dennis; Lein, Pamela J.

doi:10.1186/1471-2202-2-12

Cited by 61 publications

(26 citation statements)

References 63 publications

(59 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Artemin belongs to the subfamily of glial derived neurotrophic factor (GDNF) [32] and plays a role in neurite outgrowth and actin polymerization in DRG neurons [33]. Bmp5 has also been reported to play a role in dendritic arbor formation with its suppression resulting in reduced dendritic arbor [34], consistent with our observation of reduced MAP2 + neurites and reduced Bmp5 expression in iNs on grating topography. Mechanical cues such as substrate stiffness have been shown to impact neurite branching.…”

Section: Discussionsupporting

confidence: 86%

The effect of substrate topography on direct reprogramming of fibroblasts to induced neurons

Kulangara¹,

Adler²,

Wang³

et al. 2014

Biomaterials

View full text Add to dashboard Cite

Cellular reprogramming holds tremendous potential for cell therapy and regenerative medicine. Recently, fibroblasts have been directly converted into induced neurons (iNs) by overexpression of the neuronal transcription factors Ascl1, Brn2 and Myt1L. Hypothesizing that cell-topography interactions could influence the fibroblast-to-neuron reprogramming process, we investigated the effects of various topographies on iNs produced by direct reprogramming. Final iN purity and conversion efficiency were increased on micrograting substrates. Neurite branching was increased on microposts and decreased on microgratings, with a simplified dendritic arbor characterized by the reduction of MAP2+ neurites. Neurite outgrowth increased significantly on various topographies. DNA microarray analysis detected 20 differentially expressed genes in iNs reprogrammed on smooth versus microgratings, and quantitative PCR (qPCR) confirmed the upregulation of Vip and downregulation of Thy1 and Bmp5 on microgratings. Electrophysiology and calcium imaging verified the functionality of these iNs. This study demonstrates the potential of applying topographical cues to optimize cellular reprogramming.

show abstract

Section: Discussionsupporting

confidence: 86%

The effect of substrate topography on direct reprogramming of fibroblasts to induced neurons

Kulangara¹,

Adler²,

Wang³

et al. 2014

Biomaterials

View full text Add to dashboard Cite

show abstract

“…We were, however, not able to detect any decrease in proliferation in the two IL-6-independent cell lines JJN-3 and U-266 (data not shown). The 50% effective dose (ED 50 ) values from our experiments agree well with ED 50 values reported in other biological BMP-assays (Beck et al, 2001;Franzen and Heldin, 2001).…”

Section: There Was Variability In Bmp Receptor Expression In Five Humsupporting

confidence: 89%

“…It is not established which type I receptor BMP-5 uses, but Alk-3-Fc chimeric protein inhibited BMP-5-induced effects in cultured sympathetic neurons (Beck et al, 2001), as it did to some extent in our experiments. With the exception of a minor effect on thymidine incorporation in RPMI-8226 cells, BMP-5 gave no response in cell lines without Alk-3 transcripts, thus indirectly supporting the notion that BMP-5 may signal by ligation to Alk-3.…”

Section: Discussionmentioning

confidence: 39%

Bone morphogenetic protein-5, -6 and -7 inhibit growth and induce apoptosis in human myeloma cells

et al. 2003

View full text Add to dashboard Cite

“…Dendritic growth in sympathetic neurons is triggered by not only BMP7 but also other BMPs of the dpp and 60A subfamilies (Guo et al . 1998; Beck et al . 2001).…”

Section: Resultsmentioning

confidence: 99%

Statins decrease dendritic arborization in rat sympathetic neurons by blocking RhoA activation

Kim

Gonsiorek

Barnhart

et al. 2009

Journal of Neurochemistry

Self Cite

View full text Add to dashboard Cite

Clinical and experimental evidence suggest that statins decrease sympathetic activity, but whether peripheral mechanisms involving direct actions on post-ganglionic sympathetic neurons contribute to this effect is not known. Because tonic activity of these neurons is directly correlated with the size of their dendritic arbor, we tested the hypothesis that statins decrease dendritic arborization in sympathetic neurons. Oral administration of atorvastatin (20 mg/kg/day for 7 days) significantly reduced dendritic arborization in vivo in sympathetic ganglia of adult male rats. In cultured sympathetic neurons, statins caused dendrite retraction and reversibly blocked bone morphogenetic protein-induced dendritic growth without altering cell survival or axonal growth. Supplementation with mevalonate or isoprenoids, but not cholesterol, attenuated the inhibitory effects of statins on dendritic growth, whereas specific inhibition of isoprenoid synthesis mimicked these statin effects. Statins blocked RhoA translocation to the membrane, an event that requires isoprenylation, and constitutively active RhoA reversed statin effects on dendrites. These observations that statins decrease dendritic arborization in sympathetic neurons by blocking RhoA activation suggest a novel mechanism by which statins decrease sympathetic activity and protect against cardiovascular and cerebrovascular disease.

show abstract

Bone morphogenetic protein-5 (BMP-5) promotes dendritic growth in cultured sympathetic neurons

Cited by 61 publications

References 63 publications

The effect of substrate topography on direct reprogramming of fibroblasts to induced neurons

The effect of substrate topography on direct reprogramming of fibroblasts to induced neurons

Bone morphogenetic protein-5, -6 and -7 inhibit growth and induce apoptosis in human myeloma cells

Statins decrease dendritic arborization in rat sympathetic neurons by blocking RhoA activation

Contact Info

Product

Resources

About