Bone Morphogenetic Protein 4 Supports the Initial Differentiation of Hen (Gallus gallus) Granulosa Cells

Kim, Dong-Won; Ocón-Grove, Olga M.; Johnson, A. L.

doi:10.1095/biolreprod.113.109694

Cited by 59 publications

(62 citation statements)

References 34 publications

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“…To prove that RAP also binds to chicken ApoER2 and VLDLR, we performed ELISA-based solid-phase binding assays as described above using recombinant RAP-myc/His as ligand. As expected, both receptors bound RAP-myc/His with high affinity (15 nM for ggApoER2 [1][2][3][4][5][6][7] and ggApoER2 1-8 and 6 nM for ggVLDLR [1][2][3][4][5][6][7][8] ). As demonstrated in Fig.…”

Section: Figure 2 Expression Of Components Of the Reelin Signaling Psupporting

confidence: 71%

“…At the transition to the last growth phase, granulosa cells differentiate and express high levels of follicle-stimulating hormone receptor (6). In addition, the multicellular layer becomes a monolayer with epithelial-like properties (22).…”

Section: Discussionmentioning

confidence: 99%

“…For cloning of ggApoER2 [1][2][3][4][5][6][7][8] , ggApoER2 [1][2][3][4][5][6][7] , and ggVLDLR [1][2][3][4][5][6][7][8] , cDNA was prepared from total RNA from whole chicken follicles, thecae, or granulosa sheets as described above. The following primer pairs were used for the amplification of the indicated ligand-binding repeats: MBP-VLDLR 1-8 , 5Ј-ggt gca aaa gca aaa tgt gag ga-3Ј (forward) and 5Ј-agt taa gct ttt aag gct cat cac tcc agt cct tgc-3Ј (reverse); MBP-ApoER2 1-7 , 5Ј-gaa acg gat ttc gct tgt gac aac gg-3Ј (forward) and 5Ј-agc taa gct ttt aac cac att ctt tca gag gct cgt ca-3Ј (reverse); and MBP-ApoER2 1-8 , 5Ј-gag tgc gat aag gac cag ttc cag tgc-3Ј (forward) and 5Ј-agc taa gct ttt aac cac att ctt tca gag gct cgt ca-3Ј (reverse).…”

Section: Preparation Of Cell Extracts Sds-page Andmentioning

confidence: 99%

“…We produced recombinant fusion proteins made up of MBP and the respective ligand binding domains of chicken VLDLR containing eight ligand-binding repeats (ggVLDLR [1][2][3][4][5][6][7][8] ) and of both splice variants of chicken ApoER2 expressed in follicles (see Fig. 2B) containing seven and eight ligand-binding repeats (ggApoER2 [1][2][3][4][5][6][7] and ggApoER2 [1][2][3][4][5][6][7][8] ) and performed ligand-binding ELISAs using mouse Reelin produced by stably transfected 293HEK cells (30). As a control, we used MBP only.…”

Section: Figure 2 Expression Of Components Of the Reelin Signaling Pmentioning

confidence: 99%

“…These cells produce progesterone, which is secreted and metabolized to androgens and estrogens by cells of the theca layers of the follicle (5). The transition of undifferentiated granulosa cells to progesterone-producing cells is supported by bone morphogenic protein 4 (6). Granulosa cells may directly contribute to oocyte growth by secreting HDL-like particles, providing the oocyte with lipids for membrane synthesis (7).…”

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confidence: 99%

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Signaling by the Extracellular Matrix Protein Reelin Promotes Granulosa Cell Proliferation in the Chicken Follicle

Eresheim

Leeb

Buchegger

et al. 2014

Journal of Biological Chemistry

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Background:The control of rapid proliferation of granulosa cells during chicken oocyte growth is unknown. Results: Reelin is expressed in theca cells and triggers disabled-1 phosphorylation in granulosa cells via ApoER2 and the VLDL receptor. Conclusion:The Reelin signaling pathway stimulates granulosa cell proliferation during folliculogenesis. Significance: This work demonstrates a novel function of Reelin.

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Section: Figure 2 Expression Of Components Of the Reelin Signaling Psupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Cell Extracts Sds-page Andmentioning

confidence: 99%

Section: Figure 2 Expression Of Components Of the Reelin Signaling Pmentioning

confidence: 99%

mentioning

confidence: 99%

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Signaling by the Extracellular Matrix Protein Reelin Promotes Granulosa Cell Proliferation in the Chicken Follicle

Eresheim

Leeb

Buchegger

et al. 2014

Journal of Biological Chemistry

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Implication of SLIT3‐ROBO1/ROBO2 in granulosa cell proliferation, differentiation and follicle selection in the prehierarchical follicles of hen ovary

Qin

et al. 2018

Cell Biology International

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The SLIT/ROBO pathway has been implicated in prehierarchical follicular development of hen ovary by an intrafollicular autocrine and/or paracrine fashion. SLIT3, one of the key components of the SLIT/ROBO family, serves as a ligand that potentially interacts with the four receptors, ROBO1, ROBO2, ROBO3 and ROBO4. But the exact roles and regulatory mechanism of SLIT3 in chicken ovarian follicle development remain largely unclear. The present study was conducted to investigate the potential roles and molecular regulation of SLIT3 in granulosa cell (GC) proliferation, differentiation and follicle selection within the prehierarchical follicles of hen ovary. We found that SLIT3 interacts physically with the four ROBO receptors, but the expression of the ROBO1 and ROBO2 genes are more susceptible to the regulation of SLIT3 ligand than that of the ROBO3 and ROBO4 genes. Moreover, the siRNA‐mediated knockdown of SLIT3 in the follicular GCs leads to a significant increase in cell proliferation. Conversely, overexpression of SLIT3 results in a remarkable reduction in GC proliferation. Furthermore, the overexpressed SLIT3 has notably decreased the mRNA and protein expression levels of follicle‐stimulating hormone (FSHR), growth and differentiation factor 9 (GDF9), steroidogenic acute regulatory protein (STAR) and cytochrome P450 11A1 (CYP11A1) in the GCs. These results indicated that SLIT3 may play an inhibitory effect on GC proliferation, differentiation and follicle selection, and these suppressive actions of SLIT3 in the GC proliferation can be prohibited by the siRNA‐mediated knockdown of ROBO1 and ROBO2 receptors. The current data provide a basis for further investigation of molecular mechanisms of SLIT3‐ROBO1/2 pathway in controlling the prehierarchical follicle development of the hen ovary.

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Vasoactive intestinal peptide promotes differentiation and clock gene expression in granulosa cells from prehierarchal follicles

Kim

Johnson

2016

Molecular Reproduction Devel

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Vasoactive intestinal peptide (VIP) signaling via cyclic adenosine monophosphate (cAMP) is reported to stimulate steroidogenesis in ovarian granulosa cells from a variety of vertebrate species, including the domestic hen. Prior to follicle selection in the hen ovary (i.e., cyclic recruitment) follicle-stimulating-hormone (FSH)-induced cAMP signaling is absent within the granulosa layer until immediately following follicle selection. As a consequence, granulosa cells remain in an undifferentiated state and are unable to initiate FSH-induced steroidogenesis. VIP receptors (VPAC1 and VPAC2), like the FSH receptor, are G protein-coupled receptors, so we predicted that VIP signaling in granulosa cells is also absent in follicles that have not yet been selected into the preovulatory hierarchy. Initial studies established that mRNA encoding VPAC1 and VPAC2 are expressed within the granulosa cells throughout follicle development. Nevertheless, undifferentiated granulosa cells from prehierarchal (6-8 mm) follicles do not accumulate cAMP in response to a 4-hr incubation with chicken VIP; the capacity for such receptor signaling is attained only following selection within actively differentiating granulosa cells. VIP treatment did, however, increase expression of mRNA encoding the Gallus circadian clock protein, BMAL1-but only in granulosa cells collected from selected follicles. These findings provide evidence that, at follicle selection, the acquisition of VIP-induced cAMP cell signaling helps initiate and promote the differentiation of of granulosa cells. Furthermore, we propose that VIP signaling may regulate BMAL1 expression and, thus, a daily rhythmicity within granulosa cells of preovulatory follicles. Mol. Reprod. Dev. 83: 455-463, 2016. © 2016 Wiley Periodicals, Inc.

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Bone Morphogenetic Protein 4 Supports the Initial Differentiation of Hen (Gallus gallus) Granulosa Cells

Cited by 59 publications

References 34 publications

Signaling by the Extracellular Matrix Protein Reelin Promotes Granulosa Cell Proliferation in the Chicken Follicle

Signaling by the Extracellular Matrix Protein Reelin Promotes Granulosa Cell Proliferation in the Chicken Follicle

Implication of SLIT3‐ROBO1/ROBO2 in granulosa cell proliferation, differentiation and follicle selection in the prehierarchical follicles of hen ovary

Vasoactive intestinal peptide promotes differentiation and clock gene expression in granulosa cells from prehierarchal follicles

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