Bone-mimetic porous hydroxyapatite/whitlockite scaffolds: preparation, characterization and interactions with human mesenchymal stem cells

Bauer, L. H.; Antunović, Maja; Rogina, Anamarija; Ivanković, Marica; Ivanković, Hrvoje

doi:10.1007/s10853-020-05489-3

Cited by 23 publications

(28 citation statements)

References 67 publications

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“…This study is a continuation of our previous work [ 22 ] where bone mimetic highly porous Mg-substituted calcium phosphate scaffolds were synthesized by hydrothermal method, using calcium carbonate skeletons of cuttlefish bone. In this work, Sr-substituted and Mg- and Sr-co-substituted calcium phosphate scaffolds, with various levels of strontium and magnesium substitution were prepared using the hydrothermal method.…”

Section: Introductionmentioning

confidence: 81%

“…Cuttlefish bones ( Sepia officinalis L.) from the Adriatic Sea were used as a calcium source for the hydrothermal synthesis of ion-substituted calcium phosphates. The bone was treated as previously described [ 22 ]. In short, a dorsal shield was mechanically removed from the cuttlefish bones.…”

Section: Methodsmentioning

confidence: 99%

“…3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Sigma-Aldrich, Steinheim, Germany) assay was used to determine the potential cytotoxic effect of prepared scaffolds. The assay was performed using a human embryonic kidney (Hek293) cell line, kindly provided by Prof. Inga Urlić, Faculty of Science, University of Zagreb, by indirect contact cell culture for 72 h as previously described [ 22 ]. A fresh growth medium was added to untreated control cells as well, which were defined as 100% viable.…”

Section: Methodsmentioning

confidence: 99%

“…Unsubstituted (CaP) scaffold, Mg-substituted scaffold 1-Mg-CaP, prepared in previous work [ 22 ], and Sr and Mg-co-substituted scaffolds, 1-Mg-1-Sr-CaP and 1-Mg-5-Sr-CaP were impregnated with PCL solution in chloroform using the vacuum impregnation unit (CitoVac, Struers, Cleveland, OH, USA). Vacuum, which removes the air and ensures that polymer enters into cuttlefish bone-derived scaffolds, was used for the impregnation process by our research group on similar samples [ 28 , 29 ].…”

Section: Methodsmentioning

confidence: 99%

“…Sections were prepared and treated as described earlier [ 31 ]. Collagen type I detection process was carried out in the same manner as in our previous work [ 22 ], where a biological response was tested on magnesium substituted CaP samples.…”

Section: Methodsmentioning

confidence: 99%

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PCL-Coated Multi-Substituted Calcium Phosphate Bone Scaffolds with Enhanced Properties

et al. 2021

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Ionic substitutions within the hydroxyapatite lattice are a widely used approach to mimic the chemical composition of the bone mineral. In this work, Sr-substituted and Mg- and Sr-co-substituted calcium phosphate (CaP) scaffolds, with various levels of strontium and magnesium substitution, were prepared using the hydrothermal method at 200 °C. Calcium carbonate skeletons of cuttlefish bone, ammonium dihydrogenphosphate (NH4H2PO4), strontium nitrate (Sr(NO3)2), and magnesium perchlorate (Mg(ClO4)2) were used as reagents. Materials were characterized by means of X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Whole powder pattern decomposition refinements of XRD data indicated that increased magnesium content in the Mg- and Sr-co-substituted scaffolds was related to an increased proportion of the whitlockite (WH) phase in the biphasic hydroxyapatite (HAp)/WH scaffolds. In addition, refinements indicate that Sr2+ ions have replaced Ca2+ sites in the WH phase. Furthermore, PCL-coated Mg-substituted and Sr- and Mg-co-substituted scaffolds, with the HAp:WH wt. ratio of 90:10 were prepared by vacuum impregnation. Results of compression tests showed a positive impact of the WH phase and PCL coating on the mechanical properties of scaffolds. Human mesenchymal stem cells (hMSCs) were cultured on composite scaffolds in an osteogenic medium for 21 days. Immunohistochemical staining showed that Mg-Sr-CaP/PCL scaffold exhibited higher expression of collagen type I than the Mg-CaP/PCL scaffold, indicating the positive effect of Sr2+ ions on the differentiation of hMSCs, in concordance with histology results. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis confirmed an early stage of osteogenic differentiation.

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Section: Introductionmentioning

confidence: 81%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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PCL-Coated Multi-Substituted Calcium Phosphate Bone Scaffolds with Enhanced Properties

et al. 2021

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A Photothermal Therapy Study Based on Electrospinning Nanofibers Blended and Coated with Polydopamine Nanoparticles

Sui,

Luo,

Xiao

et al. 2023

ChemistrySelect

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The development of high‐efficiency and good biocompatible photothermal therapy platforms is conducive to avoiding the risks of nano‐preparations in vivo. The poly‐L‐lactic acid (PLLA) indicated good biodegradability and biocompatibility. Inspired by natural melanin and the extraordinary nontoxic mussel biopolymer, polydopamine nanoparticles (PDA NPs) with photothermal efficacy have attracted increasing attention. Herein, we performed the study of blended PDA/PLLA and coated PDA@PLLA electrospun nanofibers (ESNFs) for photothermal therapy. In photothermal experiment, the PDA@PLLA ESNF displayed higher light‐to‐heat conversion and photothermal stability than that of PDA/PLLA and PLLA ESNF. Both in vitro and in vivo experiments tested that three kinds of ESNF exhibited low toxicity and outstanding biocompatibility. Moreover, the PDA@PLLA ESNF showed better property for the cell proliferation due to the rough surface and good hydrophilicity. The in vivo photothermal potentiality of the PLLA, PDA/PLA and PDA@PLLA ESNF was evaluated in nude mice of inoculating the tumor, in which the PDA/PLA and PDA@PLLA ESNF significantly inhibited the tumor increasing compared to control group. Especially, the PDA@PLLA ESNF showed better photothermal therapy, which made the tumor irreversible ablate at local tumor tissue. These results stand out the usage of PDA@PLLA ESNF as potential photothermal therapy material.

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Marine-Derived Materials for Hard Tissue Repair and Regeneration

Clarke,

Walsh

2023

Handbook of the Extracellular Matrix

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Bone-mimetic porous hydroxyapatite/whitlockite scaffolds: preparation, characterization and interactions with human mesenchymal stem cells

Cited by 23 publications

References 67 publications

PCL-Coated Multi-Substituted Calcium Phosphate Bone Scaffolds with Enhanced Properties

PCL-Coated Multi-Substituted Calcium Phosphate Bone Scaffolds with Enhanced Properties

A Photothermal Therapy Study Based on Electrospinning Nanofibers Blended and Coated with Polydopamine Nanoparticles

Marine-Derived Materials for Hard Tissue Repair and Regeneration

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