Bone matrix vesicle-bound alkaline phosphatase for the assessment of peripheral blood admixture to human bone marrow aspirates

Nollet, Evelien; Craenenbroeck, Emeline M. Van; Martinet, Wim; Rodrigus, Inez; Bock, Dina De; Berneman, Zwi N.; Pintelon, Isabel; Ysebaert, Dirk; Vrints, Christiaan J.; Conraads, Viviane M.; Hoof, Viviane Van

doi:10.1016/j.cca.2015.04.013

Cited by 6 publications

(4 citation statements)

References 34 publications

(45 reference statements)

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“…The percentage of bone matrix vesicle-bound bone alkaline phosphatase (ALP) of total ALP activity, as a marker for the purity of BM aspirates, was quantified in BM serum as described previously [ 21 ]. BM aspirates in which the percentage of bone matrix vesicle-bound ALP was less than 15 % were excluded from the study for quality reasons.…”

Section: Methodsmentioning

confidence: 99%

Bone Marrow-Derived Progenitor Cells Are Functionally Impaired in Ischemic Heart Disease

Nollet

Hoymans

Rodrigus

et al. 2016

J. of Cardiovasc. Trans. Res.

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To determine whether the presence of ischemic heart disease (IHD) per se, or rather the co-presence of heart failure (HF), is the primum movens for less effective stem cell products in autologous stem cell therapy, we assessed numbers and function of bone marrow (BM)-derived progenitor cells in patients with coronary artery disease (n = 17), HF due to ischemic cardiomyopathy (n = 8), non-ischemic HF (n = 7), and control subjects (n = 11). Myeloid and erythroid differentiation capacity of BM-derived mononuclear cells was impaired in patients with underlying IHD but not with non-ischemic HF. Migration capacity decreased with increasing IHD severity. Hence, IHD, with or without associated cardiomyopathy, is an important determinant of progenitor cell function. No depletion of hematopoietic and endothelial progenitor cells (EPC) within the BM was observed, while circulating EPC numbers were increased in the presence of IHD, suggesting active recruitment. The observed myelosuppression was not driven by inflammation and thus other mechanisms are at play.Electronic supplementary materialThe online version of this article (doi:10.1007/s12265-016-9707-z) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

Bone Marrow-Derived Progenitor Cells Are Functionally Impaired in Ischemic Heart Disease

Nollet

Hoymans

Rodrigus

et al. 2016

J. of Cardiovasc. Trans. Res.

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“…BMV lumen is rich in proteins capable of chelating calcium ions, allowing for a massive increase of intracellular calcium concentration with respect to the extracellular matrix and blood concentrations [27,28,29]. Furthermore, the importance of BMV in bone marrow has been underlined by the possibility of exploiting BMP-bound alkaline phosphatase (ALP) as a marker for bone marrow (BM) aspirates in diagnostics and for research purposes [30].…”

Section: Resultsmentioning

confidence: 99%

A New Calcium Oral Controlled-Release System Based on Zeolite for Prevention of Osteoporosis

et al. 2019

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Osteoporosis, a systemic skeleton disease, can be prevented by increasing calcium levels in serum via administration of calcium salts. However, traditional calcium-based formulations have not appeared to be effective, hence the purpose of the present work has been to prepare and test in vitro/vivo a formulation able to gradually release calcium during transit over the GI tract, thus increasing bioavailability and reducing daily dose, and hence, side effects. Calcium controlled-release granules based on zeolite and Precirol® were prepared. In the best case, represented by granules sized 1.2 mm, containing 20% Precirol®, 19% zeolite, 60% calcium (granule), the release lasted ≈6 h. The release is controlled by diffusion of calcium ions through the aqueous channels forming within granules, once these come into contact with physiological fluids. Such a diffusion is hindered by the interaction of calcium ions with the negatively charged surface of the zeolite. Ovariectomy was used to make rats osteopenic. For in vivo studies, rats were divided into the following groups. Sham: not treated; ova: ovariectomized (ova); CaCl2 1.0 g: ova, treated with 1.0 g/die Ca2+; CaCl2 0.5 g: ova, treated with 0.5 g/die Ca2+; granule 1.0 g, or granule 0.5 g: ova, treated with granules equivalent to 1.0 g/die or 0.5 g/die Ca2+ in humans. Ca2+ amounts in femur bone and bone marrow, femur mechanical characteristics, and femur medullary canalicule diameter were measured and the same efficacy rank order was obtained: ova < CaCl2 0.5 g < CaCl2 1.0 g < granule 0.5 g ≈ granule 1.0 g ≈ sham. The results show promise of an effective prevention of osteoporosis, based on a controlled-rate administration of a calcium dose half that administered by the current therapy, with reduced side effects.

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“…Accordingly, a high ratio of bone-specific alkaline phosphatase to total alkaline phosphatase indicates that the aspirate is derived primarily from marrow. 155…”

Section: Bone Marrowmentioning

confidence: 99%

Needle Biopsy Adequacy in the Era of Precision Medicine and Value-Based Health Care

Pritzker

Nieminen

2019

Archives of Pathology &Amp; Laboratory Medicine

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Context.— Needle biopsy of diseased tissue is an essential diagnostic tool that is becoming even more important as precision medicine develops. However, the capability of this modality to efficiently provide samples adequate for diagnostic and prognostic analysis remains quite limited relative to current diagnostic needs. For physicians and patients, inadequate biopsy frequently leads to diagnostic delay, procedure duplication, or insufficient information about tumor biology leading to delay in treatment; for health systems, this results in substantial incremental costs and inefficient use of scarce specialized diagnostic resources. Objective.— To review current needle biopsy technology, devices, and practice with a perspective to identify current limitations and opportunities for improvement in the context of advancing precision medicine. Data Sources.— PubMed searches of fine-needle aspiration and core needle biopsy devices and similar technologies were made generally, by tissue site, and by adequacy as well as by health economics of these technologies. Conclusions.— Needle biopsy adequacy can be improved by recognizing the importance of this diagnostic tool by promoting common criteria for needle biopsy adequacy; by optimizing needle biopsy procedural technique, technologies, clinical practice, professional education, and quality assurance; and by bundling biopsy procedure costs with downstream diagnostic modalities to provide better accountability and incentives to improve the diagnostic process.

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Bone matrix vesicle-bound alkaline phosphatase for the assessment of peripheral blood admixture to human bone marrow aspirates

Abstract: The exclusive presence of BMV-bound ALP in BM could serve as a novel marker to assess purity of BM aspirates.

Cited by 6 publications

References 34 publications

Bone Marrow-Derived Progenitor Cells Are Functionally Impaired in Ischemic Heart Disease

Bone Marrow-Derived Progenitor Cells Are Functionally Impaired in Ischemic Heart Disease

A New Calcium Oral Controlled-Release System Based on Zeolite for Prevention of Osteoporosis

Needle Biopsy Adequacy in the Era of Precision Medicine and Value-Based Health Care

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