Bone marrow stromal cells induce changes in pain behavior after sciatic nerve constriction

Differential galanin upregulation in dorsal root ganglia and spinal cord after graded single ligature nerve constriction of the rat sciatic nerve

“…Using the von Frey filaments Test, it was shown that medium SLNC is the most effective in inducing mechanical allodynia, a pain-like behavior produced by normally non-noxious mechanical stimuli (Chaplan et al, 1994). Moreover, animals with medium SLNC also develop intense cold allodynia (Musolino et al, 2007), when assessed using the Choi Test (Choi et al, 1994). On the contrary, light lesions induce a modest, but still significant increase in mechanical sensitivity, while animals subjected to a strong SLNC do not show signs of mechanical allodynia, probably due to the lack of conduction of pain information through the site of severe constriction (Brumovsky et al, 2004).…”

Intrathecal bone marrow stromal cells inhibit neuropathic pain via TGF-β secretion

“…For quantitaspinal implantation of stem cells is very invasive and may be more suitable for some patients with spinal cord injury. BMSCs were also injected directly into the lesion site via the intraganglionic or intrajoint route (9,12) or administered i.v. (9,10).…”

Intra-brain microinjection of human mesenchymal stem cells decreases allodynia in neuropathic mice

“…The total RNA concentration was determined by UV spectrophotometer. The mRNA levels of the genes under analysis were measured by RT-PCR amplification, as previously reported [22]. Reverse transcriptase from avian myeloblastosis virus (AMV-RT; Promega, Madison, WI) was used.…”

“…In addition, MSCs could modulate pain generation after sciatic nerve constriction [22], although the underlying mechanisms by which MSCs exert their actions on pain behavior is still to be clarified.…”